An overview of the diversity of pathogens causing bacterial spot on tomato and pepper in Bulgaria
Keywords:
bacterial spot agents, diversity, pathotype, pepper, race, tomatoAbstract
Bacterial spot (BS) is a destructive disease affecting tomato and pepper plants. A wide diversity among the pathogens causing this disease makes them a serious threat for the tomato and pepper production worldwide, including Bulgaria, where the disease has become a major problem. To date in Bulgaria three species have been identified that infect tomato (X. vesicatoria, X. euvesicatoria and X. gardneri), and two that infect pepper (X. vesicatoria and X. euvesicatoria). Despite the research and published data, there is no general information about the BS agents on tomato and pepper in Bulgaria in respect to pathotypes, races, and the dynamics of pathogenic populations during the years. In this study we analyzed and summarized for the first time the data on the species, pathotype and race structure of the pathogenic population during the period 1999-2016. BS on tomato is caused by three species (X. vesicatoria, X. gardneri and X. euvesicatoria), and on pepper – by two species (X. euvesicatoria and X. vesicatoria). X. vesicatoria was detected and identified at the beginning of this period. This species is a dominant pathogen on tomato, whereas on pepper dominates the X.euvesicatoria species. We found a significant race diversity especially for the PT strains of X. vesicatoria and PT and P strains of X. euvesicatoria. The occurrence of new races over the past years has been registered, replacing those established at the beginning of the observed period. This indicates that the pathogenic population responsible for BS on tomato and pepper is dynamic and needs to be regularly monitored and studied, which is important to control the disease.
References
Areas, S. M., Gonçalves, R. M., Soman, J. M., Sakate, K. R., Gioria, R., da Silva Jr., T. A. F. & Maringoni, A. C. (2015). Prevalence of Xanthomonas euvesicatoria on pepper in Brazil. Journal of Phytopathology, 163 (11-12), 10501054.
Barak, J. D., Vancheva, T., Lefeuvre, P., Jones, J. B., Timilsina, S., Minsavage, G. V., Vallad, G. E. & Koebnik, R. (2016). Whole-genome sequences of Xanthomonas euvesicatoria strains clarify taxonomy and reveal a stepwise erosion of Type 3 Effectors. Frontiers in Plant Science, 7,1805. doi: 10.3389/fpls.2016.01805
Bogatzevska, N. & Sotirova, V. (1992). Occurrence of two pathotypes of Xanthomonas campestris pv. vesicatoria on tomato in Bulgaria. Report of the Tomato Genetics and Cooperative, 42, 11-12.
Bogatzevska, N. & Sotirova, V. (2001-2002). Bacterial spot of tomato in Bulgaria: pathotypes and races. Genetics and Breeding, 31 (1-2), 59-66.
Bogatzevska, N., Stoimenova, E. & Mitrev, S. (2007). Bacterial and virus diseases spread in Bulgaria and Macedonia on field and greenhouse pepper. Plant Protection Skopje, 18, 17-21.
Bouzar, H., Jones, J. B., Minsavage, G. V., Stall, R. E. & Scott, J. W. (1994a). Proteins unique to phenotypically distinct groups of Xanthomonas campestris pv. vesicatoria revealed by silver staining. Phytopathology, 84 (1), 39–44.
Bouzar, H., Jones, J. B., Stall, R. E., Hodge, N. C., Minsavage, G. V., Benedict, A. A. & Alvarez, A. M. (1994b). Physiological, chemical, serological and pathogenic analysis of a worldwide collection of Xanthomonas campestris pv. vesicatoria strains. Phytopathology, 84 (7), 663-671.
Hert, A. P., Roberts, P. D., Momol, M T., Minsavage, G. V., Tudor-Nelson, S. M. & Jones, J. B. (2005). Relative importance of bacteriocin-like genes in antagonism of Xanthomonas perforans tomato race 3 to Xanthomonas euvesicatoria tomato race 1 strains. Applied and Environmental Microbiology, 71 (7), 3581–3588.
Ignjatov, M., Gašic, K., Ivanovic, M., Ševic, M., Obradovic, A & Miloševic, M. (2010). Characterisation of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. Pesticidi i Fitomedicina, 25 (2), 139–149 (Sb).
Jones, J. B., Lacy, G. H., Bouzar, H., Minsavage, G. V., Stall, R. E. & Schaad, N. W. (2004a). Reclassification of the Xanthomonas associated with bacterial spot disease of tomato and pepper. Systematic and Applied Microbiology, 27 (6), 755762.
Jones, J. B., Lacy, G. H., Bouzar, H., Stall, R. E. & Schaad, N. W. (2004b). Bacterial spot - Worldwide distribution, importance and review. In: M. T. Mommol, P. Ji and J. B. Jones (eds.), Proceeding 1st International Symposium on Tomato Disease, 21-24 June, 2004, Orlando, Florida, USA. Acta Horticulturae, 695, 27-34.
Jones, J., Stall, R., Scott, J., Somodi, G., Bouzar, H. & Hodge, N. (1995). A third tomato race of Xanthomonas campestris pv. vesicatoria. Plant Disease, 79 (4), 395– 398.
Karov, S. (1965). Xanthomonas vesicatoria (Doidge) Dowson of pepper. Scientific Publishing, High Agricultural Institute, Plovdiv, Bulgaria, 14, 245-250.
Kebede, M., Timilsina, S., Ayalew, A., Admassu, B., Potnis, N., Minsavage, G. V., Goss, E. M., Hong, J. C., Strayer, A., Paret, M., Jones, J. B. & Vallad, G. (2014). Molecular characterization of Xanthomonas strains responsible for bacterial spot of tomato in Ethiopia. European Journal of Plant Pathology, 140 (4), 677–688.
Kizheva, Y., Vancheva, T., Hristova, P., Stoyanova, M., Bogatzevska, N. & Moncheva, P. (2011). Diversity of Xanthomonas Spp. causal agents of bacterial spot on pepper and tomato plants in Bulgaria. Biotechnology and Biotechnological Equipment, 25 (Suppl. 4), 98-104.
Kizheva, Y., Vancheva, T., Hristova, P., Stoyanova, M., Stojanovska, M., Moncheva, P. & Bogatzevska, N. (2013). Identification of Xanthomonas strains from tomato and pepper and their sensitivity to antibiotics and copper. Bulgarian Journal of Agricultural Science, 19 (2), 80–82.
Kizheva, Y., Vancheva, T., Stoyanova, M., Bogatzevska, N., Moncheva, P. & Hristova, P. (2016). 16S-23S ITS rDNA PCR-RFLP Approach as a tool for identification and differentiation of bacterial spot-causing Xanthomonas. Journal of Plant Pathology, 98 (3), 645-649.
Kizheva, Y., Urshev, Z., Rasheva, I., Vancheva, T., Hristova, P., Bogatzevska, N. & Moncheva, P. (2017). PFGE: a tool for examination of heterogeneity between the bacterial spot-causing xanthomonads of tomato plant in Bulgaria. Zeitshrift für Naturforshung C, 73 (7-8), 257-264.
Kovachevski, I. (1936). New parasitic fungi in Bulgaria. Trudove na Balgarskoto Prirodoizpitvatelno Druzhestvo, 7, 13–24 (Bg).
Kyeon, M. S., Son, S. H., Noh, Y. H., Kim, Y. E., Lee, H. I. & Cha, J. S. (2016). Xanthomonas euvesicatoria causes bacterial spot disease on pepper plant in Korea. Plant Pathology Journal, 32 (5), 431440.
Ma, X., Lewis Ivey, L. & Miller, S. (2011). First report of Xanthomonas gardneri causing bacterial spot of tomato in Ohio and Michigan. Plant Disease, 95 (12), 1584.
Minsavage, G. V., Dahlbeck, D., Whalen, M. C., Kearney, B., Bonas, U., Staskawicz, B. J. & Stall, R. E. (1990). Gene-for-gene relationships specifying disease resistance in Xanthomonas campestris pv. vesicatoria-pepper interactions. Molecular Plant-Microbe Interactions, 3 (1), 41–47.
Mitrev, S. (2001). Phytopathogenic bacteria on pepper in Macedonia, Working paper, PSI Institute of Southern Crops, Strumica, 90 pp. (Mc).
Obradovic, A., Mavridis, A., Rudolph, K., Janse, J. D., Arsemijevic, M., Jones, J. B., Minsavage, G. V. & Wang, J. F. (2004). Characterization of PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. European Journal of Plant Pathology, 110 (3), 285–292.
O’Garro, L.W. & Tudor, S. (1994). Contribution of four races of Xanthomonas campestris pv. vesicatoria to bacterial spot in Barbados. Plant Disease, 78 (1), 88–90.
O’Garro, L. W., Gore, J. P. & Ferguson, E. (1999). Races of Xanthomonas campestris pv. vesicatoria overcoming the gene Bs2 for bacterial spot resistance in pepper, prevalent on Capsicum chinense in Barbados and Grenada and weakly pathogenic on bell pepper an tomato in the field. Plant Pathology, 48 (5), 588-594.
Potnis, N., Timilsina, S., Strayer, A., Shantharaj, D., Barak, J. D., Paret, M. L., Vallad, G. E. & Jones, J. B. (2015). Bacterial spot of tomato and pepper: diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge. Molecular Plant Pathology, 16 (9), 907–920.
Quezado-Duval, A. M., Leite, R. P., Truffi, D. & Camargo, L. E. A. (2004). Outbreaks of bacterial spot caused by Xanthomonas gardneri on processing tomato in central-west Brazil. Plant Disease, 88 (2), 157-161.
Sahin, F. & Miller, S. A. (1998). Resistance in Capsicum pubescens to Xanthomonas campestris pv. vesicatoria pepper race 6. Plant Disease, 82 (7), 794–799.
Schwartz, A. R., Potnis, N., Timilsina, S., Wilson, M., Patané, J., Martins, Jr. J., G. V. Minsavage, G. V., Dahlbeck, D., Akhunova, A., Almeida, N., Vallad, G. E., Barak, J. D., White, F. F., Miller, S. A., Ritchie, D., Goss, E., Bart, R. S., Setubal, J. C., Jones, J. B. & Staskawicz, B. J. (2015). Phylogenomics of Xanthomonas field strains infecting pepper and tomato reveals diversity in effector repertoires and identifies determinants of host specificity. Frontiers in Microbiology, 6 (Article 535), 1-17. doi:10.3389/fmicb.2015.00535
Stall, R. E., Jones, J. B. & Minsavage, G. V. (2009). Durability of resistance in tomato and pepper to xanthomonads causing bacterial spot. Annual Review of Phytopathology, 47 (1), 265–284.
Stoyanova, M., Vancheva, T., Moncheva, P. & Bogatzevska, N. (2014). Differentiation of Xanthomonas spp. causing bacterial spot in Bulgaria based on Biolog System. International Journal of Microbiology, 7-0, doi:http://dx.doi.org/10.1155/2014/495476.
Timilsina, S., Jibrin, M. O., Potnis, N., Minsavage, G. V., Kebede, M., Schwartz, A., Bart, R., Staskawicz, B., Boyer, C., Vallad, G. V., Pruvost, O., Jones, J. B. & Goss, E. M. (2015). Multilocus sequence analysis of xanthomonads causing bacterial spot of tomato and pepper plants reveals strains generated by recombination among species and recent global spread of Xanthomonas gardneri. Applied and Environmental Microbiology, 81 (4), 1520–1529.
Timilsina, S., Kara, S., Jacques, M. A., Potnis, N., Minsavage, G. V., Vallad, G. E., Jones, J. B. & Fischer-Le Saux, M. (2018). Reclassification of Xanthomonas gardneri (ex Šutič 1957) Jones et al. 2006 as a later heterotypic synonym of Xanthomonas cynarae (Trébaol et al., 2000) and description of X. cynarae pv. cynarae and X. cynarae pv. gardneri based on whole genome analyses. International Journal of Systematic and Evolutionary Microbiology, DOI:10.1099/ijsem.0.003104.
Tudor-Nelson, S. M., Minsavage, G. V., Stall, R. E. & Jones, J. B. (2003). Bacteriocin-like substances from tomato Race 3 strains of Xanthomonas campestris pv. vesicatoria. Phytopathology, 93 (11), 1415–1421.
Vancheva, T., Stoyanova, M., Tasheva-Terzieva, E., Bogatzevska, N. & Moncheva, P. (2018). Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper. Brazilian Journal of Microbiology, 49 (Suppl.1), 246-259.
Vancheva, T., Stoyanova, M., Tatyozova, M., Bogatsevska, N. & Moncheva, P. (2014). Sub-species diversity of Xanthomonas euvesicatoria Bulgarian and Macedonian strains from pepper. Biotechnology and Biotechnological Equipment, 28 (4), 592601.
Ward, H. P. & O’Garro, L. W. (1992). Bacterial spot of pepper and tomato in Barbados. Plant Disease, 76 (10), 1046–1048.
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