Characterization of Pepper Line with Cytoplasmic Male Sterility and Maintainer Line

Authors

  • Velichka Todorova Maritsa Vegetable Crops Research Institute, Plovdiv, Agricultural Academy - Sofia, Bulgaria Author
  • Yanina Arnaoudova Maritsa Vegetable Crops Research Institute, Plovdiv, Agricultural Academy - Sofia, Bulgaria Author
  • Veselina Nikolova Maritsa Vegetable Crops Research Institute, Plovdiv, Agricultural Academy - Sofia, Bulgaria Author

Keywords:

Capsicum; CMS; electron microscopy; pepper; traits

Abstract

Pepper line K 587 (Srfrf) with cytoplasmic male sterility (CMS) and line K 588 (Nrfrf) – maintainer of sterility forming small hot fruits were studied on the experimental plot of the Maritsa Vegetable Crops Research Institute, Plovdiv during the period 2006-2007. These lines were estimated by the significant morphological traits: plant and stem height, number of branches form first order, fruit length, fruit diameter at the bottom, pericarp thickness, average fruit weight. The CMS line is described with higher plants (129.95 cm), greater number of branches from first order (3.75), shorter (6.62 cm) and narrow (1.10 cm) fruits with thinner pericarp (1.57 mm) and with smaller average fruit weight (6.19 g). The parietal layer of the two lines was studied by electronic-microscopy analysis. It was established that in the CMS line strong vacuolization of the tapetal cells occurred before the meiotic division while in the line-maintainer this process was observed in telophase second.

References

Лидански, Т. 1988. Статистически методи в биологията и селското стопанство. Земиздат, София, 375 с.

Терзийски, Д. и С. Карагеоргиев. 1989. Ръководство по основи на електронната микроскопия. ВСИ „В. Коларов” – Пловдив, с. 93

Плохинский, Н. А. 1970. Биометрия. Колос, Москва, 334 с.

Dash, S. S, S. Kumar and J. N. Singh. 2001. Cytomor phological characterization of a nuclear male sterile line of chilli pepper (Capsicum annuum L.). Caryologia, 66: 365-371

Dong, L. H., G. Zh. Hui, W. H. Min, Wei H. and L. M. Hui. 2006. Cyto-morfological observation of the microsporo genesis of male sterility materials in pepper Capsicum an nuum L. Acta Botanica Boreali-Occidentalia Sinica, 26 (9): 1842-1845

Gurung, T., S. Techawongstein, B. Suriharn and S. Techawongstein. 2011. Growth, yield and capsaicinoid con tents of 14 cultivars of hot pepper (Capsicum spp.) at two elevations of Thailand. Sabrao Journal of Breeding and Genetics, 43 (2), 130-143

Horner, H. T. and M. Rogers. 1974. A comparative light and electron microscopic study of microsporogenesis in male-fertile and cytoplasmic male sterile pepper Capsicum annuum. Can. J. Bot., 52: 435-441

Horner, H. and R. G. Palmer. 1995. Mechanisms of genic male sterility. Crop Science, 35: 1527-1535

Jo, Y. D. 2007. Comparative analysis of DNA structure containing cox2 and atp6-2 genes between fertile and CMS chilli pepper (Capsicum annuum L.). MS Thesis, Seoul Natl. Univ. Seoul, Korea.

Krouleva, M. and S. Daskalov. 1978. Comparative cy toembryological studies of cytoplasmatic and gene male sterility in the pepper Capsicum annuum. C. R. Acad. Sci. Bulg., t. 31, 8: 1073-1076

Li, Y. Y., Y. Wei, R. H. Zhang and J. Lu. 2006. Cyto logical investigation on microsporogenesis of male sterility of pepper. Bulletin of Botanical Research, 26, 4: 411-415

Min, W. K., B. D. Kim, S.G. Kim and S. H. Lee. 2011. Development of new molecular markers for the identification of male sterile cytoplasm in peppers (Capsicum annuum L.). Korean Journal of Horticultural Science and Technology, 29, 1, p. 53-60

Wang, S. B., X. D. Luo, L. F. Dai, CH. T. Qian, J. F. Chen, J. B. Liu and B. G. Pan. 2004. Meotic observation and male gametes development in cytoplasmic male sterile of pepper (Capsicum annuum L.). Acta Horticulturae Sinica, 31, 6, p. 807-810

Published

28.04.2013

How to Cite

Characterization of Pepper Line with Cytoplasmic Male Sterility and Maintainer Line. (2013). Bulgarian Journal of Crop Science, 50(2), 30-33. https://agriacad.eu/ojs/index.php/bjcs/article/view/3798