CHARACTERISATION OF SIX ISOLATED BACTERIAL STRAINS FROM GENUS BACILLUS APPLICABLE IN ORGANIC FARMING
Keywords:
Bacillus, phosphatase activity, PisumsativumAbstract
The aim of this study was to determine the activity of six isolated strains from genus Bacillus applicable in organic farming. These strains were cultivated in two different liquid nutrient media in order to examine their growth. Monitoring of the growth was carried out in order to achieve high concentrations of active cells. The results showed that the better medium for achieving high concentrations of active cells for all tested strains was PDG. The enzymatic potential of six isolated strains was assessed by the API ZYM system (BioMérieux, France) by semi-quantitative scale from 0 to 5. The strains showed phosphatase (acid and alkaline) and phospho-hydrolase activities in the range 4–5. For confi rmation of the results qualitative analysis for determination of phosphatase activity by the method of Pikovska was performed. The effect of the strains on the growth of test plant Pisumsativum was studied (in vivo). The results showed that when Pisumsativum was inoculated with strains T 3, T 10 and T 18, the fresh root biomass and fresh shoot biomass increased significantly.
References
Cherry, J. R. and A. L. Fidantsef, 2003. Directed evolution of industrial enzymes: an update. CurrOpinBiotechnol., 14: 438–443.
Chen, X. H., A. Koumaoutsi, R. Scholz and R. Borriss, 2009. More than anticipated-production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42. J MolMicrobiol-Biotechnol., 16:14–24.
Cuero, R.G., R. Waniska, J. Fajardo, G. Osuji and E. Duffus, 1991. Enhancement of Phytoalexin and Chitosanase by Chitosan in Germinating Peanut: Biocontrol of Toxigenic Fungi and Mycotoxins. In: 5th Int. Conf. on Chitin and Chitosan (C.J. Brine, P.A. Sandford, and J.P. Zikakis, eds.), Princeton, NJ, Elseviere Pub. Co. pp. 419–432.
Gutierrez-Manero, F. J., B. Ramos-Solano, A. Probanza, J. Mehuachi, F. R. Tadeo and M. Talon, 2001. The plant growth-promoting rhizobacteria Bacillus pumilusand Bacillus licheniformisproduce high amounts of physiologically active gibberellins. Physiol. Plant., 111: 206–211.
Hurek, T. and B. Reinhold -Hurek, 2003. Azoarcus spp. strain BH72 as a model for nitrogen fi xing grass endophytes. J Biotechnol., 106: 169.
Idris, E. E. S., D. J. Iglesias, M. Talon and R. Borriss, 2007. Tryptophan-dependent production of Indole-3-Acetic Acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42. Mol Plant Microbe Interact., 20: 619–626.
Kloepper, J. W. and M. N. Schroth, 1978. Plant growth promoting rhizobacteria on radishes. In: Proceedings of IV International Conference on Plant Pathogenic Bacteria. pp. 879–882.
Kumar, A., S. Saini, A. Prakash and B. N. Johri, 2009. Influence of cultivation practices on phenotypic and genotypic diversity of antagonistic rhizobacteria isolated from soybean (Glycine max L.). In: Abstracts, 1st Asian PGPR Congress for Sustainable Agriculture, Hyderabad. pp. 118.
Richardson, A. E., J. M. Barea, A. M. McNeill and C. Prigent-Combaret, 2009. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil, 321: 305–339.
Sinchaikul, S., B. Sookkheo, S. Topanuruk, H. F. Juan, S. Phutrakul and S. T. Chen, 2002. Bioinformatics, functional genomics, and proteomics study of Bacillus sp. J Chromatogr B AnalytTechnol Biomed Life Sci., 771: 261–287.
Zhdan-Pushkina, N. Movchan and S. Shtelkunova, 1974. Practical Microbiology, Leningrad, 78 pp. (Ru).
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Bulgarian Journal of Agricultural Science
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
