PCR-RFLP analysis of MSTN gene in Karakachan sheep breed

Authors

  • M. BOZHILOVA-SAKOVA University of Forestry, Department of Genetics and Selection, Agronomy Faculty, 10, Kl. Ohridski bl.,1756 Sofia, Bulgaria Author
  • I. DIMITROVA University of Forestry, Department of Genetics and Selection, Agronomy Faculty, 10, Kl. Ohridski bl.,1756 Sofia, Bulgaria Author
  • A. TENEVA University of Forestry, Department of Genetics and Selection, Agronomy Faculty, 10, Kl. Ohridski bl.,1756 Sofia, Bulgaria Author
  • N. PETROV Institute of Soil Science “Nikola Poushkarov” 7, Shousse Bankya Str., 1080 Sofia, Bulgaria Author

Keywords:

Karakachan sheep breed, MSTN gene, PCR-RFLP method

Abstract

The present study was conducted in order to investigate and identify the allelic variants of myostatin (MSTN) gene associated with meat traits in sheep. Blood samples were taken from a population of 25 animals of Bulgarian breed Karakachan sheep – 22 ewes and 3 rams. Genomic DNA was extracted and myostatin genotypes were estimated by means of PCR amplification and PCR-RFLP method. The PCR products were digested with HaeIII restriction enzyme. In the total population of sheep polymorphism was not found. The MSTN locus was found to be monomorphic – only the allele m and the genotype mm were detected.

References

Azari, M. A., E. Dehnavi, S. Yousseli and L. Shahmohamadi, 2012. Polymorphism of calpastatin, calpain and myostatin genes in native Dalaghsheep in Iran. Slovak J. Anim. Sci., 45(1): 1-6.

Bellinge, R. H. S., D. A. Liberles, S. P. Iaschi, P. A. O’Brien and G. K. Tay, 2005. Myostatin And Its Implications On Animal Breeding: A Review. Anim. Genet., 36: 1–6.

Dehnavi, E., M. Azari, S. Hasani, M. R. Nassiry, M. Mohajer, A. Ahmadi, L. Shahmohamadi and S. Yousefi, 2012. Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep. Biotechnology Research International, Volume 2012, Article ID 472307, 5 pages, DOI: 10.1155/2012/472307.

Dimitrova, I., M. Bozhilova-Sakova, N. Stancheva and T. Tzonev, 2016. Molecular Analysis of Ovine Myostatin Gene (MSTN) in Northeast Bulgarian Merino Sheep Breed Using PCR-RFLP Method. Bulg. J. Agric. Sci. (in print).

Elkorshy, N., K. Mahrous and L. Salem, 2013. Genetic polymorphism detection in four genes in Egyptian and Saudi sheep breeds. World Applied Science Journal, 27(1): 33-43.

Georgieva, S., D. Hristova, I. Dimitrova, N. Stancheva and M. Bozhilova-Sakova, 2015. Molecular analysis of ovine calpastatin (CAST) and myostatin (MSTN) genes in Synthetic Population Bulgarian Milk sheep using PCR-RFLP. Journal of BioScience and Biotechnology, 4(1): 95-99.

Jamshidi, S., S. Karani and M. Goudarzi, 2014. Study polyporphism myostatin gene in Mehraban’s sheep using PCR-RFLP method. Sci,Int (Lahore), 26(3): 1129-1135.

Kijas, J. W., R. McCulloch, J. E. H. Edwards, V. H. Oddy, S. H. Lee, and J. van der Werf, 2007. Evidence for multiple allele effecting muscling and fatness at the Ovine GDF8 locus. BMC Genetics. Doi:10.1186/1471-2156-8-80.

McPherron, A. C. and S. J. Lee, 1997. Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94(23): 12457-12461.

Mirhoseini, S. Z. and J. Zare, 2012. The role of Myostatin on growth and carcass raits and its application in animal breeding, Life Sci J, 9(3); 2353-2357.

Nakev, G., P. Stamberov, I. Dimitrova, N. Stancheva, G. Georgieva, D. Hristova, G. Angelov, T. Mehmedov, K. Genova and A. Teneva, 2013. Growth and development of skeletal muscle in connection with the expression of the myostatin gene (MSTN). In: Proceedings of the 10th International Symposium Modern Trends in Livestock Production, October 2-4, 2013, pp. 640-647.

Shafey, H. I., K. F. Mahrous, M. S. Hassanane, M. Abdel Mordy and H. E. Rushdi, 2014. Genetic polymorphism of myostatin and insulin-like growth factor binding protein-3 genes in Egyptian sheep breeds, Global Veterinaria, 13(3): 419-424, DOI: 10.5829.

Sontegard, T. S., G. A. Rohrer and T. P. L. Smith, 1998. Myostatin maps to porcine chromosome 15 by linkage and physical analysis, Anim. Genet., 29(1): 19-22.

Soufy, B., M. R. Mohammad Abadi, K. Shojaeian, A. Baghizadeh, S. Ferasaty, N. Askari and O. Dayani, 2009. Evaluation of Myostatin gene polymorphism in Sanjabi sheep by PCR-RFLP method. Animal Science Reserches Tabriz Univ., 19(1): 81-89.

Tambasco, D. D., C. C. P. Paz, M. Tambasco-Studart, A. P. Pereira, M. M. Alencar, A. R. Freitas, L. L. Coutinho, L. U. Packer and L. C. A. Regitano, 2003. Candidate genes for growth traits in beef cattle cross Bostaurus × Bosindicus. Journal of Animal Breeding and Genetics, 120: 51-56.

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Published

31.12.2016

Issue

Vol. 22 No. Supplement 1 (2016): Bulgarian Journal of Agricultural Science

Section

Problems of Science and Practice

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Copyright (c) 2016 Bulgarian Journal of Agricultural Science

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How to Cite

BOZHILOVA-SAKOVA, M., DIMITROVA, I., TENEVA, A., & PETROV, N. (2016). PCR-RFLP analysis of MSTN gene in Karakachan sheep breed. Bulgarian Journal of Agricultural Science, 22(Supplement 1), 115–117. https://agriacad.eu/ojs/index.php/bjas/article/view/6550