CALLUS CULTURES AND INDIRECT REGENERATION OF RUSCUS HYPOGLOSSUM IN VITRO
Keywords:
genome size, isoenzymes, micropropagation, rhizome, RuscaceaeAbstract
Ruscus hypoglossum L. is a highly valued ornamental plant collected mainly from the wild and threatened in several European
countries. Slow growth and low germination rates hamper its cultivation. Micropropagation has been considered advantageous
for rapid production of planting material and ex situ conservation of Ruscus species. Callus cultures of R. hypoglossum
were induced on TDZ containing medium and indirect shoot regeneration rate was evaluated on different medium composition.
TDZ ensured higher regeneration rates, caused shoot, and cladode alterations in regenerates persisting throughout the
cultivation. Callus induction was optimal at 30 g.l–1 sucrose and dropped down with the increase of sucrose concentration. Increase
of the sucrose (15–60 g.l–1) infl uenced positively shoot proliferation. DNA content of the morphologically altered plants
was not significantly different. Isozyme profile patterns demonstrated loss of isoform bands and/or induction of new ones.
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