Effect of plant growth regulators on chloroplast ultra structure in Lamium album plantlets
Keywords:
in vitro-regeneration, indole-3-butyric acid, medicinal plant, N6-benzyladenine, ransmission electron microscopy, white dead nettleAbstract
Lamium album L. stem nodal segments were grown on Murashige and Skoog’s medium without growth regulators (control) and on media supplemented with 0.2 mg l-1 N6-benzyladenine, 0.5 mg l-1 indole-3-butyric acid or both plant growth regulators. The plastid apparatus of in vitro formed leaflets was studied by transmission electron microscopy. The cytokinin N6-benzyladenine negatively affected the chloroplast formation when was applied individually or in combination with the auxin indole-3-butyric acid. On the contrary, IBA in concentration of 0.5 mg l-1 was appropriate for development of chloroplasts with regular shape and well organized internal membrane system thus providing for the regeneration capacity in vitro.
References
Aloni, R., 1995. The induction of vascular tissues by auxin and cytokinin. In: Davies, P. J., (Editor) Plant hormones, physiology, biochemistry and molecular biology. Dordrecht, The Netherlands: Kluwer Academic Publishers, pp. 531-546.
Arigita, L., B.Fernández, A. González and R. Sánchez Tamés, 2005. Effect of the application of benzyladenine pulse on organogenesis, acclimatisation and endogenous phytohormone content in kiwi explants cultured under autotrophic conditions. Plant Physiology and Biochemistry, 43: 161–167.
Colijn, C. M., P. Sijmons, J. N. M. Mol, A. J. Kool and H. J. J. Nijkamp, 1982. Light and benzylaminopurine induce changes in ultrastructure and gene expression in plastids of Petunia hybrida cell cultures. Current Genetics, 6: 129-135.
Debergh, P., J. Aitken-Christie, D. Cohen, B. Grout, S. Arnold, R. Zimmerman and M. Ziv, 1992. Reconsideration of the term “vitrification” as used in micropropagation. Plant Cell, Tissue and Organ Culture, 30: 135-140.
Deccetti, S. F. C., A. M. Soares, R. Paiva and E. M. Castro, 2008. Effect of the culture environment on stomatal features, epidermal cells and water loss of micropropagated Annona glabra L. plants. Scientia Horticulturae, 117: 341–344.
Fontes, M. A., W. C. Otoni, S. M. B. Carolino, S. H. Brommonschenkel, E. P. B. Fontes, M. Fári and R. P. Louro, 1999. Hyperhydricity in pepper plants regenerated in vitro: involvement of BiP (Binding Protein) and ultrastructural aspects. Plant Cell Reports, 19: 81–87.
Gopi, C., Y. Nataraja Sekhar and P. Ponmurugan, 2006. In vitro multiplication of Ocimum gratissimum L. through direct regeneration. African Journal of Biotechnology, 5: 723-726.
Hazarika, B. N., 2006. Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae, 108: 105–120.
Jausoro, V., B. E. Llorente and N. M. Apóstolo, 2010. Structural differences between hyperhydric and normal in vitro shoots of Handroanthus impetiginosus (Mart. ex DC) Mattos (Bignoniaceae). Plant Cell, Tissue and Organ Culture, 101: 183–191
Laetsch, W. M. and D. A. Stetler, 1965. Chloroplast structure and function in cultured tobacco tissue. American Journal of Botany, 52: 798-804.
Lee, N., H. Y. Wetzstein and H. E. Sommer, 1985. Effects of quantum flux density on photosynthesis and chloroplast ultrastructure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L. towards improved acclimatization and field survival. Plant Physiology, 78: 637-641.
Louro, R. P., A. V. Dos Santos and R. D. Machado, 1999. Ultrastructure of Eucalyptus grandis x Eucalyptus urophylla. I. Shoots cultivated in vitro in multiplication and elongationrooting media. International Journal of Plant Sciences, 160: 217-227.
Lucchesini, M., G. Monteforti, A. Mensuali-Sodi and G. Serra, 2006. Leaf ultrastructure, photosynthetic rate and growth of myrtle plantlets under different in vitro culture conditions. Biologia Plantarum, 50: 161-168.
Magyar-Tábori, K., J. Dobránszki, J. A. Teixeira da Silva, S. M. Bulley, I. Hudák, 2010. The role of cytokinins in shoot organogenesis in apple. Plant Cell, Tissue and Organ Culture, 101: 251–267.
Majada, J. P., M. A. Fal, F. Tadeo and R. Sánchez-Tamés, 2002. Effects of natural ventilation on leaf ultrastructure of Dianthus caryophyllus L. cultured in vitro. In Vitro Cellular and Developmental Biology-Plant, 38: 272-278.
Mazari, A. and E. L. Camm, 1993 Effect of cytokinins on plastid development and photosynthetic polypeptides during organogenesis of Pinus ponderosa Dougl. cotyledons cultured in vitro. Plant Cell, Tissue and Organ Culture, 33: 81-89.
Mohamed, M. A.-H. and A. A. Alsadon, 2011. Effect of vessel type and growth regulators on micropropagation of Capsicum annuum. Biologia Plantarum, 55: 370-374.
Oliveira, L. M., R. Paiva, J. R. F. Santana, E. Alves, R. C. Nogueira and F. D. Pereira, 2008. Effect of cytokinins on in vitro development of autotrophism and acclimatization of Annona glabra L. In Vitro Cellular and Developmental Biology-Plant, 44: 128–135.
Olmos, E. and E. Hellín, 1998. Ultrastructural differences of hyperhydric and normal leaves from regenerated carnation plants. Scientia Horticulturae, 75: 91-101.
Paduch, R., G. Matysik, M. Wójciak–Kosior, M. Kandefer–Szerszeñ, A. Skalska–Kamiñska, M. Nowak–Kryska and P. Niedziela, 2008 Lamium album extracts express free radical scavenging and cytotoxic activities. Polish Journal of Environmental Studies, 17: 569-580.
Paek, K.-Y. and E.-J. Hahn, 2000. Cytokinins, auxins and activated charcoal affect organogenesis and anatomical characteristics of shoot-tip cultures of lisianthus [Eustoma grandiflorum (Raf.)Shinn]. In Vitro Cellular and Developmental Biology-Plant 36: 128–132.
Polanská, L., A. Vičánková, M. Nováková, J. Malbeck, P.I. Dobrev, B. Brzobohaty, R. Vaňková and I. Macháčková, 2007. Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco. Journal of Experimental Botany, 58: 637–649.
Serret, M. D. and M. I. Trillas, 2000. Effects of light and sucrose levels on the anatomy, ultrastructure, and photosynthesis of Gardenia jasminoides Ellis leaflets cultured in vitro. International Journal of Plant Sciences, 161: 281-289.
Sudriá C., J. Palazón, R. Cusidó, M. Bonfill, M. T. Piñol and C. Morales, 2001. Effect of benzyladenine and indolebutyric acid on ultrastructure, glands formation, and essential oil accumulation in Lavandula dentata plantlets. Biologia Plantarum, 44: 1-6.
Tasheva, K. and G. Kosturkova, 2013. Role of biotechnology for protection of endangered medicinal plants. In: Petre M. (Editor) Environmental Biotechnology - New Approaches and Prospective Applications. ISBN: 978-953-51-0972-3, InTech, DOI: 10.5772/55024.
Toma, I., C. Toma and G. Ghiorghita, 2004. Histo-anatomy and in vitro morphogenesis in Hyssopus officinalis L. (Lamiaceae). Acta Botanica Croatica, 63: 59–68.
Valyova, M. S., M. A. Dimitrova, Y. A. Ganeva, V. M. Kapchina-Toteva, Z. P. Yordanova, 2011. Evaluation of antioxidant and free radical scavenging potential of Lamium album L.growing in Bulgaria. Journal of Pharmacy Research, 4: 945-947.
Wetzstein, H. Y. and H. E. Sommer, 1982. Leaf anatomy of tissuecultured Liquidambar styraciflua (Hamamelidaceae) during acclimatization. American Journal of Botany 69: 1579-1586.
Wilhelmova, N. and J. Kutík, 1995. Influence of exogenously applied 6-benzylaminopurine on the structure of chloroplasts and arrangement of their membranes. Photosynthetica 31: 559-570.
Downloads
Published
Issue
Section
License
Copyright (c) 2013 Bulgarian Journal of Agricultural Science
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
