COMPARISON OF SALINITY AND DROUGHT STRESS EFFECTS ON SOME MORPHOLOGICAL AND PHYSIOLOGICAL PARAMETERS IN ONION (ALLIUM CEPA L.) DURING EARLY GROWTH PHASE
Keywords:
abiotic stress, drought, proline, salinity, toleranceAbstract
This study was conducted to investigate the effects of drought and salinity on four onion (Allium cepa L.) cultivars (Texas Early Grano, Kantartopu-3, Besirli-77 and Akgun-12) in the early plant growth phase. Seeds were germinated in peat material. After 21 days of sowing, seedlings were transferred to plastic pots (1.6 L). The plants have been grown in vermiculite by “substrate culture” technique. After ten days of transplanting, irrigation was stopped gradually for drought stress. For this aim, three different levels of irrigation were used. (D0 was 90% of field capacity, D1 was 70% of field capacity, and D2 treatment was 40% of feed capacity). In order to determination of effects of salinity on onion plants, 0. 50 mM and 125 mM NaCl were added to nutrition solution. To determine the effect of salinity and drought stress factors, tolerance indexes based on plant neck diameter (mm), leaf diameter (mm), plant length (cm), amount of leaves, leaf water potential (%) and waxy on leaves were measured as morphological parameters. At the same time, amount of proline, the concentrations of total chlorophyll (µg/ml), chlorophyll-a (µg/ml), chlorophyll-b (µg/ml) carotenoids (µg/ml) and chlorophyll-a/b were investigated as physiological parameters. Cultivars showed different responses to drought and salinity. The significant varietal differences were observed for the proline amount, chlorophyll-a, leaf water potential index and leaf amount under drought stress; and for the proline amount, chlorophyll-a and leaf diameter under salinity stress.
References
Adams, E. and L. Frank, 1980. Metabolism of proline and the hydroxyprolines. Ann. Rev. Biochem., 49: 1005:1061.
Bates, L. S., R. P. Waldren and I. D.Teare,1973. Rapid determination of free proline for water-stress studies. Plant Soil, 3 (9): 205-207.
Chinnusamy, V., A. Jagendorf and J. Zhu, 2005. Understanding and improving salt tolerance in plants. Crop Science Society of America, 45: 437-448.
Djekoun, A. and C. Planchon, 1991. Water status effect on dinitrogen fixation and photosynthesis in soybean. Agron. J., 83: 316-322.
Dragland, S., 1974. Nitrogen and water requirements in onions. Forskning og Forsok Landbruket, 26: 93-113.
Drinkwater, W. O. and B. E. Janes, 1955. Effects of irrigation and soil water on maturity, yield, and storage of two onion hybrids. Proc. Am. Soc. Hort. Sci., 66: 267-279.
El-Baz, F. K., A. A. Mahamed and A. A. Aly, 2003. Development of biochemical markers for salt stres tolerance in cucumber plants. Pakistan Journal of Biological Sciences, 6 (1-2).
FAO, 2012. FAO Agricultural Statistical Database. http://faostat.org
Goudarzi, M. and H. Pakniyat, 2008. Comparison between salt tolerance of various cultivars of wheat and maize. J. Applied Sci., 8: 2300-2305.
Hanci, F., E. Cebeci and Y. Y. Mendi, 2012. Effects of NaCl and CaCl2 on Germination performance of some local onion (Allium cepa L.) cultivars in Turkey. Acta Hort., ISHS, 960: 203-209.
Hoagland, D. R. and D. I. Arnon, 1950. The Water Culture Method for Growing Plants without Soil, Circ. 347, Berkeley, CA California Agricultural Experiment Station.
Joshi, N. and P. Sawant, 2012. Response of onion (Allium cepa L.) seed germination and early seedling development to salt level. International Journal of Vegetable Science, 18 (1): 3-19.
Khan, M. A., 2002. Halophyte seed germination: success and pitfalls. In: A. M. Hegazi et al. (Eds.) International Symposium on Optimum Resource Utilization in Salt Affected Ecosystems in Arid and Semi-Arid Regions, Desert Research Centre, Cairo, pp. 346-358.
Koriem, S. O., M. M. A. El-Koliey and M. F. Wahba, 1994. Onion bulb production from ‘‘shandwell’’ sets as affected by soil moisture stress. Assuit J. Agric. Sci., 25: 185-193.
Lichtenthaler, H. K. and A. R. Wellburn, 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11: 591-592.
Maas, E. V. and G. J. Hoffman, 1977. Crop salt tolerance - Current assessment. J. Irrig. Dram. Div., ASCE, 103: 115-134.
Mafakheri, A., A. Siosemardeh, B. Bahramnejad, P. C. Struik, Y Sohrabi, 2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science, 4 (8): 580-585.
Mangal, J. L., R. K. Sing and A. C. Yadava, 1991. Selection for increased salinity in onion seed crops and effects on other characters. Veg. Sci., 18: 140-145.
Pesarakli, M., 199. Handbok of Plant and Crop Stres, 2nd Ed., Marcel Deker Inc, New York, 247-259.
Sanders, D. C. 1997. Vegetable Crop Irrigation North Carolina State University. http://www.ces.ncsu.edu/hil/hil-33-e.html.
Sidari, M., C. Santonoceto, U. Anastasi, G. Preiti and A. Muscolo, 2008. Variations in four genotypes of lentil under nacl- salinity stress. Am. J. Agric. Biol. Sci., 3 (1): 410-416.
Singh, R. and R. B. Alderfer, 1966. Effects of soil moisture stress at different periods of growth of some vegetable crops. Soil Sci., 101 (1): 69-80.
Stino, K. R., A. M. Abdul-Fattah, A. Abdel-Salam and M. M. Abdel Gawod, 1972. Salinity effects on the growth of some onion varieties. Bul. Dirasat Inst., 22: 167-174.
Tatar, O. and M. N Gevrek, 2008. Influence of water stress on proline accumulation, lipid peroxidation and water content of Wheat. Asian Journal of Plant Sciences, 7: 409-412.
Vendruscolo, A. C. G., I. Schuster, M. Pileggi, C. A. Scapim, H. B. C. Molinari, C. J. Marur and L. G. C Vieira, 2007. Stressinduced synthesis of proline confers tolerance to water deficit in transgenic wheat. Journal of Plant Physiology, 164: 1367-1376.
Wannamaker, M. J. and L. M. Pike. 1987. Onion responses to various salinity levels. J. Amer. Soc. Hort. Sci., 112: 49-52.
Zgallai, H., K. Steppe and R. Lemeur, 2005. Photosynthetic, physiological and biochemical responses of tomato plants to polyethylene glycol-induced water deficit. Journal of Integrative Plant Biology, 47: 1470-1478.
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