IN VITRO CULTIVATION AND EX VITRO ADAPTATION OF NEPETA NUDA SSP. NUDA CORRELATION BETWEEN REGENERATION POTENTIAL, LEAF ANATOMY, AND PLASTID PIGMENTS

Authors

  • D. DRAGOLOVA Sofia University “St. Kliment Ohridski”, Faculty of Biology, Department of Plant Physiology, BG - 1164 Sofia, Bulgaria Author
  • M. STEFANOVA Sofia University “St. Kliment Ohridski”, Faculty of Biology, Department of Botany, BG - 1164 Sofia Author
  • M. DIMITROVA Sofia University “St. Kliment Ohridski”, Faculty of Biology, Department of Plant Physiology, BG - 1164 Sofia, Bulgaria Author
  • D. KOLEVA Sofia University “St. Kliment Ohridski”, Faculty of Biology, Department of Botany, BG - 1164 Sofia Author
  • M. ZHIPONOVA Sofia University “St. Kliment Ohridski”, Faculty of Biology, Department of Plant Physiology, BG - 1164 Sofia, Bulgaria Author
  • V. KAPCHINA-TOTEVA Sofia University “St. Kliment Ohridski”, Faculty of Biology, Department of Plant Physiology, BG - 1164 Sofia, Bulgaria Author

Keywords:

assimilation parenchyma, carotenoids, chlorophyll, cryopreservation, medicinal plant

Abstract

The leaf anatomy and the amount of the plastid pigments in in vivo plants, in vitro plants, in vitro regenerated after cryopreservation plants (cryo plants) and ex vitro adapted plants of Nepeta nuda ssp. nuda were examined as markers for the assessment of the regeneration potential of the species. The anatomical study showed that under in vitro conditions leaf with thin lamina and uniformly structured assimilation parenchyma was formed. The leaf of in vivo and ex vitro plants was equally structured - bifacial. It was found that the amount of the plastid pigments is greater in in vitro, cryo and ex vitro plants as compared to in vivo plants. The highest value was measured in cryo plants. The results indicated a high regeneration potential of N. nuda ssp. nuda during in vitro cultivation and ex vitro adaptation.

References

Alim, A., I. Goze, A. Cetin, A. D. Atas, S. A. Cetinus and N. Vural, 2009. Chemical composition and in vitro antimicrobial and antioxidant activities of the essential oil of Nepeta nuda L. subsp. albiflora (Boiss.) gams. African Journal of Microbiology Research, 3 (8): 463-467.

Aly, H., M. Ebrahim, H. Metawaa, E. A. Hosni and F. Ebrahim, 2010. In vitro and in vivo evaluation of the antidiabetic effect of different extracts of Nepeta cataria in streptozotocin induced diabetic rats. Journal of American Science, 6: 364-386.

Apóstolo, N. M. and B. E. Llorente, 2000. Anatomy of normal and hyperhydric leaves and shoots of in vitro grown Simmondsia chinesis (link) schn. In Vitro Cellular & Developmental Biology - Plant, 36 (4): 243-249.

Arnon, D. T., 1949. Copper enzymes in isolated chloroplast: polyphenoloxidase in Beta vulgaris. Plant Physiol., 241: 1-15.

Danova, K., B. Nikolova-Damianova, R. Denev and Yu. Markovska, 2012. Impact of preculture on short- and long-term in vitro recovery of the biosynthetic potential and enzymatic and non-enzymatic antioxidant defense of Hypericum rumeliacum Boiss. after cryostorage. Plant Growth Regul., 68: 447-457.

De Pooter, H. L., B. Nicolai, L. F. De Buyck, P. Goetghebeur and N. M. Schamp, 1987. The essential oil of Nepeta nuda. Identification of a new nepetalactone diastereoisomer. Phytochemistry, 26: 2311-2314.

Dousseau, S., A. A. Alvarenga, E. M. Castro, R. P. Soares, E. B. Emrich and L. A. Melo, 2008. Leaf anatomy of Tabebuia serratifolia (Vahl) Nich. (Bignoniaceae) propagated in vitro, in vivo and during the acclimatization. Ciênc. Agrotec., 32 (6): 1694-1700.

Georgieva, E., D. Petrova, Zh. Yordanova, V. Kapchina-Toteva, E. Čellárová and G. Chaneva, 2014. Influence of cryopreservation on the antioxidative activity of in vitro cultivated Hypericum species. Biotech. & Biotech. Equi., 28 (5): 863-870.

Gkinis, G., B. Bozin, N. Mimica-Dukic and O. Tzakou, 2010. Antioxidant activity of Nepeta nuda L. ssp. nuda essential oil rich in nepetalactones from Greece. J Med Food., 13 (5): 1176-1181.

Handjieva, N. V., S. S. Popov and L. N. Evstatieva, 1996. Constituents of essential oils from Nepeta cataria L., N. grandiflora M. B. and N. nuda L. J. Essent. Oil Res., 8: 639-643.

Hazarika, B. N., 2006. Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae, 108: 105-120.

Hussain, J., N. Jamila, S. A. Gilani, G. Abbas and S. Ahme, 2009. Platelet aggregation, antiglycation, cytotoxic, phytotoxic and antimicrobial activities of extracts of Nepeta juncea. African Journal of Biotechnology, 8 (6): 935-940.

Ivanov, A., P. Sane, M. Krol, G. Gray, A. Balseris, L. Savitch, G. Oquist and N. Huner, 2006. Acclimation to temperature and irradiance modulates PSII charge recombination. FEBS Lett., 580: 2797-2802.

Jarda, L., V. Cristea, C. Crăciun and S. Tripon, 2011. Comparative study of Dianthus giganteus subsp. banaticus leaves anatomy in different growing conditions. I. Light microscopy aspects. Annals of the Romanian Society for Cell Biology, 16 (1): 331-347.

Knöss, W., 1999. Marrubium vulgare (white horehound): in vitro cultured and the production of diterpene marrubiin and other secondary metabolites. In: Y. P. S. Bajaj (Ed.) Biotechnology in Agriculture and Forestry 43. Medicinal and Aromatic plants XI, p. 423.

Mayer, J. L. S., L. L. F. Ribas, C. Bona and M. Quoirin, 2008. Anatomia comparada das folhas e raízes de Cymbidium Hort. (Orchidaceae) cultivadas ex vitro e in vitro. Acta Botanica Brasilica, 22 (2): 323-332.

McKinney, 1941. Absorption of light by chlorophyll solutions. J. Biol. Chem., 140: 315-332.

Mehrabani, M., A. Asadipour and A. S. Saber, 2004. Chemical constituents of the essential oil of Nepeta depauperata Benh. from Iran. Daru., 12 (3): 98-100.

Mills, D., 2009. Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets. Biologia Plantarum, 53 (3): 415-421.

Murashige, T. and F. A. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.

Partelli, F., H. Vieira, A. Viana, P. Batista-Santos, A. Rodrigues, A. Leitão and J. Ramalho, 2009. Cold impact on photosynthetic related parameters in coffee genotypes. Pesqui Agropecu Bras., 44: 1404-1415.

Sáez, P. L., L. A. Bravo, K. L. Sáez, M. Sánchez-Olate, M. I. Latsague and D. G. Ríos, 2012. Photosynthetic and leaf anatomical characteristics of Castanea sativa: a comparison between in vitro and nursery plants. Biologia Plantarum, 56 (1): 15-24.

Stefanova, M., D. Koleva, T. Ganeva and M. Dimitrova, 2011. Effect of plant growth regulators on the regeneration of in vitropropagated Lamium album L. plants. Journal of Pharmacy Research, 4 (7): 1982-1985.

Zhao, Y., Y. Zhou and B. W. W. Grout, 2006. Variation in leaf structures of micropropagated rhubarb (Rheum rhaponticum L.) PC49. Plant Cell, Tissue and Organ Culture, 85:115-121.

Zobayed, S. M. A., F. Afreen-Zobayed, C. Kubota and T. Kozai, 1999. Stomatal characteristics and leaf anatomy of potato plantlets cultured in vitro under photoautotrophic and photomixotrophic conditions. In Vitro Cellular and Developmental Biology-Plant, 3 (5): 183-188.

Downloads

Published

30.10.2015

Issue

Vol. 21 No. 5 (2015): Bulgarian Journal of Agricultural Science

Section

Plant Science

License

Copyright (c) 2015 Bulgarian Journal of Agricultural Science

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite

DRAGOLOVA, D., STEFANOVA, M., DIMITROVA, M., KOLEVA, D., ZHIPONOVA, M., & KAPCHINA-TOTEVA, V. (2015). IN VITRO CULTIVATION AND EX VITRO ADAPTATION OF NEPETA NUDA SSP. NUDA CORRELATION BETWEEN REGENERATION POTENTIAL, LEAF ANATOMY, AND PLASTID PIGMENTS. Bulgarian Journal of Agricultural Science, 21(5), 1027-1032. https://agriacad.eu/ojs/index.php/bjas/article/view/5502