THE HISTOPATHOLOGICAL INVESTIGATION OF LIVER IN EXPERIMENTAL ANIMALS AFTER SHORT-TERM EXPOSURES TO PESTICIDES
Keywords:
acetamiprid, histopathological alterations, mice, mixture of pesticide, propinebAbstract
Pesticides are one of the most dangerous pollutants for human and environment health due to their toxic effects and accumulation features. Acetamiprid (Acm), a neonicotinoid insecticide, and Propineb (Pro), a dithiocarbamate fungicide, are also widely used to control sucking insects and fungal infections on crops, respectively. The present study was undertaken to investigate the histopathology of Acm, Pro and the mixture of them were investigated on the liver tissue of mice. Fifteen groups were prepared for the histopathological examinations. Three of these were the positive, and the other three were the negative control groups. Nine of these were experiment groups. The mice were intaperitoneally treated to three different concentrations of pesticidesfor short-term (24 and 48 h) treatments. The histopathological effects of pesticides on the liver tissue of mice were determined by light microscopy. After 24 and 48 h, all concentrations of Acm significantly induced formation of sinusoidal dilatation. Vacuolar degeneration was also significantly observed at the lowest concentration (0.625 μg/ml) of Acm for 48 h. Pro (25 and 50 μg/ml) significantly caused vacuolar degeneration in the hepatocytes and sinusoidal dilatation in the parenchyma for 24 h. It was also observed that the mixture of Acm and Pro significantly induced formation of vacuolar degeneration, vascular dilatation and sinusoidal dilatation in almost all of the concentrations both 24 and 48 h when compared with the negative control. Furthermore, the mixture of Acm and Pro also significantly produced synergistic effects when the results of individually applied pesticides compared to their mixture at the same concentrations. These results led to the conclusion that Acm and Pro may have destructive effects on the liver tissue of mice. So, the use of these pesticides should be under control in agricultural fields.
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