IDENTIFICATION OF BANANA ACCESSIONS SAMPLED FROM SUBTROPICAL REGION OF TURKEY USING SRAP MARKERS
Keywords:
molecular identification, Musa, mutationAbstract
This study was conducted to estimate genetic relationships and natural somatic mutations among selected banana genotypes from different region of Turkey via SRAP molecular markers. Ninety-six banana genotypes including 39 ‘Dwarf Cavendish’, 18 ‘Grande Nain’, 28 ‘Azman’ and 11 other bananas were evaluated. A total of 102 bands were obtained from 19 SRAP primer combinations and 86 (85.5%) of them were polymorphic. Number of bands per primer combination was 5.3 whereas
polymorphic bands per primer combinations were 4.5. Genetic similarity of 96 accessions varied between 0.63–1.00. It was determined genetic variation among the accessions which may allow new selections for breeding programs. Considerable variation was also found among genotypes within the same banana cultivar and it was very important for banana breeding and germplasm evaluation. It can be concluded that SRAP markers could readily be used to estimate genetic relationships among banana genotypes and natural somatic mutations.
References
Bhat, K. V., R. L. Jarret and R. S. Rana, 1995. DNA profi ling of banana and plantain cultivars using random amplifi ed polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers. Electrophoresis, 16: 1736–1745.
Brown, N., S. Venkatasamy, G. Khittoo, T. Bahorun and S. Jawaheer, 2009. Evaluation of genetic diversity between 27 banana cultivars (Musa spp.) in Mauritius using RAPD markers. African J. of Biotechnology, 8: 1834–1840.
Creste, S., T. Neto R. Vencovsky, S. O. Silva and A. Figueira, 2004. Genetic diversity of Musa diploid and triploid accessions from the Brazilian banana breeding program estimated by microsatellite markers. Genetic Res. and Crop Evol., 51: 723–733.
Das, B. K., R. C. Jena and K. C. Samal, 2009. Optimization of DNA isolation and PCR protocol for RAPD analysis of banana/ plantain (Musa spp.). Int. J. of Agriculture Sci., 1: 21–25.
Dice, L. R., 1945. Measures of the amount of ecologic association between species. Ecology, 26: 297–302.
Donini, P. and A. Sonnino, 1998. Induced mutation in plant breeding: Current status and future outlook In: S. M. Jain, D. S. Brar, B. S. Ahloowalia (Eds). Somaclonal Variation and Induced Mutations in Crop Improvement, pp. 255–291.
Doyle, J. J. and J. L. Doyle, 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13–15.
Eckstein, K., C. Fraser, J. Husselmann and N. Temple-Murray, 1998. The evaluation of promising new banana cultivars. Acta Horticulturae, 490: 57–69.
FAO, 2011. http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID= 567#ancor
Gubbuk, H., M. Pekmezci, A. Onus and M. Erkan, 2004. Identifi cation and selection of superior banana phenotypes in the cultivar Dwarf Cavendish using agronomic characteristics and RAPD markers. Pakistan Journal of Botany, 36: 331–342.
Gubbuk, H. and M. Pekmezci, 2001. Determination of genetic variations among banana clones via RAPD markers. Bahce, 30: 71–79 (Tr).
Gulsen, O., R. C. Shearman, K. P. Vogel, D. J. Lee, P. S. Baenziger, T. M. Heng-Moss and H. Budak, 2005. Nuclear genome diversity and relationships among naturally occurring buffalograss genotypes determined by SRAP markers. Hortscience, 4: 537–541.
Mattos, L. A., E. P. Amorim, V. B. O. Amorim, K. O. Cohen, C. A. S. Ledo and S. O. Silva, 2010. Agronomical and molecular characterization of banana germplasm. Pesquisa Agropecuária Brasileira, 45: 146–154.
Khayat, E., A. Duvdevani, A. Shchukin and E. Lahav, 1998. Banana improvement program at Rahan Meristem. Acta Horticulturae, 490: 71–78.
Lakshmanan, V., S. R. Venkataramareddy and N. Bhagyalakshmi, 2007. Molecular analysis of genetic stability in longterm micropropagated shoots of banana using RAPD and ISSR markers. Electronic Journal of Biotechnology, 10: 107–113.
Li, G. and C. F. Quiros, 2001. Sequence related amplifi ed polymorphism (SRAP) a new marker system based on a simple pcr reaction: Its application to mapping and gene tagging in Brassica. Theoretical and Appl. Genetics, 103: 455–461.
Lu, Y., X. Zhang, J. Pu, Y. Qi and Y. Xie, 2011. Molecular assessment of genetic identity and genetic stability in banana cultivars (Musa spp.) from China using ISSR makers. Australian J. of Crop Science, 5: 25–31.
Mantel, N., 1967. The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209–220.
Marak, C. K. and M. A. Laskar, 2010. Analysis of phenetic relationship between Citrus indica Tanaka and a few commercially important citrus species by ISSR markers. Scientia Horticulturae, 124: 345–348.
Opara, U. L., D. Jacobson and N. A. Al-Saady, 2010. Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classifi cation by phylogenetic, hierarchical clustering and principal component analyses. J. of Zhejiang University – Science B (Biomedicine & Biotechnology), 11: 332–341.
Onguso, J. M., E. M. Kahangi, D. W. Ndiritu and F. Mizutani,
Genetic characterization of cultivated bananas and plantains in Kenya by RAPD markers. Scientia Horticulturae, 99: 9–20.
Phothipan, S., B. Silayoi, K. Wanichkul and S. Apisitwanich, 2005. Genetic relationship among bananas in AA, AAB and BB groups using random amplifi ed polymorphic DNA (RAPD) and sequence related amplifi ed polymorphism (SRAP) techniques. Kasetsart Journal, 39: 703–710.
Pinar, H., C. Turkay, N. Denli, M. Ünlü and M. Bircan, 2011. Potantial of Banana Production in Turkey. GAP VI. Agriculture Congress (in press), 09–12 May, 2011, Sanliurfa, Turkey.
Retnoningsih, A., R. Megia and A. Hartana, 2011. Microsatellite markers for classifying and analysing genetic relationship between banana cultivars in Indonesia. Acta Horticulturae, 897:
–159.
Rohlf, F. J., 2000. NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System, Version 2.1. Exeter Publications, New York, USA.
Scholz, M. and J. Selbig, 2006. Visualization and analysis of molecular data. In: W. Weckwerth (Ed), Metabolomics: Methods and Protocols. Methods in Molecular Biology, pp. 87–104.
Simmonds, N. W. and K. Shepherd, 1955. The taxono my and origins of cultivated bananas. J. of the Linnean Society of London, Botany, 55: 302–312.
Smith, M., A. Wiley, C. Searle, P. Langdon, B. Schaffer and K. Pegg, 1998. Micropropagated bananas are more susceptible to Fusarium wilt than plants grown from conventional material. Australian J. of Agricultural Research, 49: 1133–1139.
Ude, G., M. Pillay, D. Nwakanma and A. Tenkouano, 2002. Analysis of genetic diversity and sectional relationships in Musa using AFLP markers. Theoretical and Applied Genetics, 104: 1239–1245.
Uzun, A., O. Gulsen, G. Kafa and U. Seday, 2009. Field performance and molecular diversifi cation of lemon selection. Scientia Horticulturae, 120: 473–478.
Uzun, A., O. Gulsen, U. Seday, M. Bircan and K. U. Yilmaz, 2010a. SRAP based genetic analysis of some apricot cultivars. Romanian Biotechnological Letters, 15: 5396–5404.
Uzun, A., O. Gulsen, T. Yesiloglu, Y. A. Kacar and O. Tuzcu, 2010b. Distinguishing grapefruit and pummelo accessions using ISSR markers. Czech J. of Genetics and Plant Breeding, 46: 170–177.
Venkatachalam, L., R. V. Sreedhar and N. Bhagyalakshmi, 2008. The use of genetic markers for detecting DNA polymorphism, genotype identifi cation and phylogenetic relationships among banana cultivars. Molecular Phylogenetics and Evolution, 47: 974–985.
Youssef, M., A. C. James, R. Rivera-Madrid, R. Ortiz and R. M. Escobedo-Gracia Medrano, 2011. Musa genetic diversity revealed by SRAP and AFLP. Molecular Biotechnology, 47: 189–199.
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