Efficiency of lactic acid bacteria as the potent degradative microorganism to digest the total mixed ration in vitro
Keywords:
lactic acid bacteria, digestibility, total mixed rationAbstract
The objective of this research was to investigate the efficiency of lactic acid bacteria on in vitro digestibility of total mixed ration (TMR) containing pineapple plant silage 55%, rice straw 15%, cassava ship 5.6%, soybean meal 12.6%, palm kernel pulp l4.2%, rice bran 5.6%, premixed 0.15% and molasses 2%. Guinea grass (Panicum maximum) was used for isolation and identification of microorganisms from silage. Treatments were used as TMR treated with lactic acid bacteria as follows: Treatment 1 – non treated; Treatment 2 – TMR treated with Lactobacillus pantarum; Treatment 3 – TMR treated with Pediococcus pentosaceus; Treatment 4 – TMR treated with Pediococcus acidilactici; Treatment 5 – TMR treated with L. pantarum + P. pentosaceus + P. acidilactici. The treated TMR were mixed and put in polyethylene bags and stored at ambient temperature for 7 days before analysis. The in vitro digestibility test used pepsin cellulase in vitro digestibility method. The results showed that there were significant differences (P < 0.01) of the nutritive value of TMR by proximate analysis and fiber analysis which treated lactic acid bacteria. For the digestibility, results showed that the TMR treated with LAB using pepsin cellulase in vitro digestibility which applied LP, PP, PA and LPP could improve the digestibility of TMR, which significantly increased in digestibility of TMR (P < 0.01). The rate of increase and decrease of digestibility and fiber in TMR treated with LAB showed an increase in dry matter and a decrease in fiber. Therefore, it was suggested that TMR treated with lactic acid bacteria of TMR may possible to develop as biological animal feed for ruminant.
References
AOAC (Association of Official Analytical Chemists) (1995). Official Methods of Analysis of the Association of Official Analytical Chemists.16th ed. Washington, DC.
Cai, Y., Benno, Y., Ogawa, M., Ohmomo, S., Kumai, S., & Nakase, T. (1998). Infl uence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Applied and Environmental Microbiology, 64(8), 2982-2987.
Catchpoole, V. R., & Henzell, E. F. (1971). Silage and silage-making from tropical herbage species. Herbage Abstracts, 41(3), 213-221.
De Man, J. C., Rogosa, D., & Sharpe, M. E. (1960). A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology, 23(1), 130-135.
Guo, X. S., Undersander, D. J., & Combs, D. K. (2013). Effect of Lactobacillus inoculants and forage dry matter on the fermentation and aerobic stability of ensiled mixed-crop tall fescue and meadow fescue. Journal of Dairy Science, 96(3), 1735-1744.
Hayward, A. C. (1957). Detection of gas production from glucose by heterofermentative lactic acid bacteria. Microbiology, 16(1), 9-15.
Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., & Williams, S. T. (1994). Bergey’s manual of determinative microbiology. Williams and Wilkins, Maryland, Maryland, USA.
Kandler, O., & Weiss, N. (1986). Regular, nonsporing gram-positive rods. In: Bergey’s manual of systematic bacteriology (vol. 2, pp. 1208-1234). The Williams and Wilkins Co., Baltimore.
Mayed, T. (2016). Effect of Pak Chong 1 napier grass (Pennisetum purpureum x Pennisetum americanum) silage total mixed rations on goat production and its adoption by goat smallholders. J. Academic Services (Prince of Songkla University), 27(1), 116-122.
McDonald, P., Henderson, N., & Heron, S. (1991). The biochemistry of silage. 2nd ed., Marlow Bottom, Chalcombe.
McLeod, M. N., & Minson, D. J. (1978). The accuracy of the pepsin-cellulase technique for estimating the dry matter digestibility in vivo of grasses and legumes. Animal Feed Science and Technology, 3(4), 277-287.
Nocek, J. E., Steele, R. L., & Braund, D. G. (1985). Effect of mixed ration nutrient density on milk of cows transferred from high production group. Journal of Dairy Science, 68(1), 133139.
Sasivimol, C. A., & Swetwiwathana, A. (2005). Utilization and detection of lactic acid bacteria in food. KMITL Agri. J., 23, 88-101.
Smith, J. F. (1988). Pretreatment of lignocellulosics for edible fungi. In: Treatment of lignocellulosic for edible fungi (F. Zadrazil and P. Reiniger, eds.). Elsevier Applied Science Publisher Ltd.,
Wichai Suphalucksana, Kasem Soytong
Barking England.
South Bend Medical Foundation (2010). Catalase test protocol. South Bend Medical Foundation, South Bend, Inc.
Stiles, M. E., & Holzapfel, W. H. (1997). Lactic acid bacteria of foods and their current taxonomy. International Journal of Food Microbiology, 36(1), 1-29.
Suphalucksana, W., & Soytong, K. (2017). Lactic acid bacteria and enzyme production in silage of guinea grass (Panicum maximum). Bulg. J. Agri. Sci. 23(1), 86-91.
Van Soest, P. J. (1963). Use of detergents in the analysis of fi brous feeds. 2. A rapid method for the determination of fi ber and lignin. Journal of the Association of Offi cial Agricultural Chemists, 46, 825.
Van Soest, P. J. (1964). Symposium on nutrition and forage and pastures: new chemical procedures for evaluating forages. Journal of Animal Science, 23(3), 838.
Van Soest, P. J., & Moore, L. A. (1965). New chemical methods for analysis of forages for the purpose of predicting nutritive value. In Proceedings of the 9th International Grassland Congress (pp. 783-789).
Van Soest, P., & Robertson, J. (1979). Systems of analysis for evaluating fi brous feeds. In Standardization of analytical methodology for feeds, Proceedings of a workshop, IDRC, Ottawa, ON, CA.
Wallace, R. J., Wallace, S. J., McKain, N., Nsereko, V. L., & Hartnell, G. F. (2001). Infl uence of supplementary fi brolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro. Journal of Animal Science, 79(7), 1905-1916.
Yang, J., Cao, Y., Cai, Y., & Terada, F. (2010). Natural populations of lactic acid bacteria isolated from vegetable residues and silage fermentation. Journal of Dairy Science, 93(7), 31363145.
Zhang, J. G., Cai, Y., Kobayashi, R., & Kumai, S. (2000). Characteristics of lactic acid bacteria isolated from forage crops and their effects on silage fermentation. Journal of the Science of Food and Agriculture, 80(10), 1455-1460.
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