Comparative analysis of genetic diversity of bread wheat genotypes based on protein and DNA markers
Keywords:
wheat, gliadin, protein markers, ISSR markers, genetic diversityAbstract
Genetic diversity of 32 local and 23 introduced bread wheat (Triticum aestivum L.) genotypes was studied using protein and inter simple sequence repeat (ISSR) markers. A total of 26 bands were amplified using 5 ISSR primers. The number of polymorphic bands varied from 1 to 3, averaged 2.2. The average genetic diversity for the whole bread wheat collection based on ISSR data was 0.57. Polymorphism rate of protein markers were superior to ISSR markers. The highest number of patterns was noted at α and ω sections, with 44 and 42 patterns, respectively. Genetic diversity index was high for all studied sections. The maximum diversity was observed at α section (0.968), followed by ω section (0.963). Mantel test did not reveal significant correlation between dendrograms created, based on protein and DNA markers, suggesting that both set of markers explore genetic variation differently. In the ISSR dendrogram some tendencies were observed in grouping of genotypes according to their botanical varieties, while no relationship was found between grouping of genotypes and their origin or botanical variety based on protein markers.
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