MICROBIOLOGICAL STUDY OF TRADITIONAL CHEESE PRODUCED IN RUGOVA REGION OF KOSOVO
Keywords:
traditional cheese, Rugova region, microbial activity, LABAbstract
Total mesophilic bacteria, anaerobic bacteria, lactic acid bacteria, enterobacteria as well as yeast and moulds were investigated during the production of the traditional Rugova cheese without using any starter culture. This type of cheese has been produced and consumed by the local population in the Rugova region for centuries, and is important for their economy. Samples from different stages of production (raw milk, curd and cheese during maturation on day 1, 8 and 14) were collected from local farms in the Rugova region, Kosovo, during winter. After sample collection and their serial dilution, samples were plate on different media and incubated at the appropriate temperature. According to identical morphology and physiology the number of 52 isolates obtained from de Man, Rogosa, and Shape (MRS), and M17 agar was reduced to ten Gram-positive and catalase negative strains. These strains were subjected to API 50 CHL fermentation profiling. There was a variety of microbial groups detected in all stages of cheese production, with the dominant microbial groups being Lactococcus (from 6.44 log cfu/ml in milk to 8.39 log cfu/gr in cheese D14) and Lactobacillus (from 5.79 log cfu/ml in milk to 7.58 log cfu/gr in cheese D14). Enterobacter and yeast and moulds were found in lower amounts compared to the other microbes in all stages. The level of Leuconostoc was approximately the same as Lactobacillus. Almost all microbial groups were low in curd and started to increase from day one of cheese manufacturing. Three of the ten isolates were identified as Lactococcus lactis ssp. lactis, four as Lactobacillus plantarum (2 isolates) and L. curvatus (2 isolates). One isolate of each of Lactobacillus paracasei ssp. paracasei, L. brevis and L. pentosus were identified. These results confirm the competitiveness of high numbers of LAB during fermentation of this cheese.
References
Abegaz, K., 2014. Isolation, characterization and identifi cation of lactic acid bacteria involved in traditional fermentation of borde, an Ethiopian cereal beverage. International Journal of Food and Nutrition science., 1 (1): 007-015.
Ahmadova, A., S.D. Totorov, I.H. Sfaxi, Y. Choiset, H. Rabesona, S. Messaoudi, A. Kulivev, B.D.G. de M. Franco, J. Chobert and T. Haertle., 2013. Antimicrobial and antifungal activity of Lactobacillus curvatus strain isolated from Azerbaijani cheese. Anaerobe (Elsevier), 20: 42-49.
Alegria, A., S. Delgado, C. Roces, B. López and B. Mayo., 2010. Bacteriocins produced by wild Lactococcus lactis strains isolated from traditional, starter-free cheeses made of raw milk. Int. J. Food Microbiol., 143: 61-66.
Alegria, A., P. Szczesny, B. Mayo, J. Bardowski and M. Kowalczyk., 2012. Biodiversity in Oscypec, a traditional Polish cheese, determine by culture-dependent and-independent approaches. Appl. Environ. Microbiol., 78 (6): 1890. DOI: 10.1128/AEM. 06081-11.
Burentegusi, T. Miyamoto, S. Nakamura, Y. Nosaka and A. Aoisi., 2002. Identifi cation of lactic acid bacteis isolated from fermented mare’s milk ‘Chigee’ in inner Mongolia, China. Animal science Journal., 73: 441-448.
Caric, M., 1993. Ripened cheese varieties native to the Balkan countries, in: Fox P.F. (ed.), Cheese. Chemistry, Physics and Microbiology, Vol. 2, 2nd ed., Chapman & Hall, London, UK, pp. 263-279.
Cheriguene, A., F. Chougrani., A.M.A. Bekada, M.El Soda and A. Bensoltane., 2007. Enumeration and identifi cation of lactic microfl ora in Algerian goat’s milk. African Journal of Biotecnology., 6 (15): 1854-1861.
Europian Commission, 2006. On the protection of geographical indications and designations of origin for agricultural product and food stuffs. Commision regulation EC 510/06. Off. J. Eur. Union, L93: 12-25.
Gudina, E.J., V. Rocha., J.A. Teixeira and L.R. Rodrigues, 2010. Antimicrobial and antiathesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20. Applied Microbiology, 50: 419-424.
Guetouache, M and B. Guessas, 2015. Characterization and Identifi cation of lactic acid bacteria isolated from traditional cheese (Klila) prepared from cow’s milk. African Journal of Microbiology Research, 9 (2): 71-77.
Gulitz, A., J. Stadie, M. Wenning, M.A. Ehrmann and R.F. Vogel, 2013. The microbial diversity of water kefi r. International Journal of Food Microbiology, 151 (3) 284-288.
Hassanzadazar, H. and A. Ehsani., 2013. Phenotipic characterization of lactic acid bacteria isolated from traditional Koopeh cheese. Global Veterinaria, 10 (2) 148-152.
Hurst, A. and D.G. Hoover, 1993. Nisin, In: P.M. Davidson and A.L. Branen (ed.), Antimicrobials in Foods, 2nd ed., Marcel Dekker Inc., New York, N.Y, pp. 369-394.
Mikulec, D.P. and L. Jovanovic, 2005. Microbiological study of fresh white cheese (a Serbian craft variety). Applied Ecology and Environmental Research, 4 (1): 129-134.
Quigle, L., O. O’Sullivan, C. Stanton, T.P. Beresfor, R.P. Ross, G.F. Fitzgerald and P.D. Cotter, 2013. The complex microbiota of raw milk. FEMS Microbiol Rev, 37: 664-698.
Randazzo, C.L., S. Torriani, A.D.L. Akkermans, W.M. de Vos and E.E. Vaughan, 2002. Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Sicilian Cheese as Evaluated by 16S rRNA Analysis. Appl. Environ. Microbiol., DOI: 10.1128/AEM. 68.4.1882-1892.
Rilla, N., B. Martinez, T. Delgaro and Ana Rodriguez, 2003. Inhibition of clostridium tyrobutyricum in Vidiago cheese by Lactococcus lactic ssp lactis IPLA 729, a nisin Z producer. Internacional Journal of Food Microbiology, 85 (1-2): 23-33.
Sudun, Wulijideligen, K. Arakawa, M. Miyamoto and T. Miyamoto, 2013. Interaction between lactic acid bacteria and yeasts in airag, an alcoholic fermented milk. Animal Science Journal, 84: 66-74.
Tajabadi1, N., M. Mardan, N. Saari1, S. Mustafa, R. Bahreini and M.Y.A. Manap, 2013. Identifi cation of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee. Brazilian Journal of Microbiology, 44 (3) 717-722.
Topisirovic, L., M. Kojic, D. Fira, N. Golic, I. Strahinic and J. Lozo, 2006. Potential of lactic acid bacteria isolated from specifi c natural niches in food production and preservation. International Journal of Food Microbiology, 112: 230-235.
Turchi, B., R. Novolani, F. Fratini, F. Pedonese, V.V. Ebani and D. Cerri, 2011. Caciotta della Garfagnana cheese: selection and evaluation of autochthonous mesophilic lactic acid bacteria as starter culture. Italian Journal of Animal Science, volume 10:e22.
Vehapi I. and K. Kurteshi, 2013. Microbiologycal analysis of homemade cheese. Bulgarian Journal of Veterinary Medicine, 16 (suppl. 1) 231-233.
Waki, N., M. Matsumoto, Y. Fukui and H. Suganuma., 2014. Effects of probiotics Lactobacillus brevis KB290 on incidence of infl uenza infection among schoolchildren: an open label pilot study. Letters in Applied Microbiology, 59 (6): 565-571.
Zago, M., M.E. Fornasari, D. Carminati, P. Burns, V. Suàrez, G. Vinderola, J. Reinheimer and G. Giraffa, 2011. Characterization and probiotic potential of Lactobacillus plantarum strains isolated from cheeses. Food Microbiology, 28: 1033-1040.
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