ULTRASOUND-ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM POLYGONUM MULTIFLORUM THUNB. ROOTS
Keywords:
antioxidant, Polygonum multifl orum Thunb, polyphenol, solvent, ultrasoundAbstract
The goal of this study is to examine the infl uence of type of solvent (acetone, ethanol, methanol, and water), raw solvent/ material ratio (20/1–60/1, v/w), solvent concentration (30–80%, v/v), temperature (30–70°C), time of extraction (10-30 mins) and power consumption of 550 W on the extraction yield of total phenolic compounds (TPC) and antioxidant activity (AC) from Polygonum multifl orum Thunb. roots. TPC and AC were determined by the Folin–Ciocalteu assay and DPPH assay. The optimal conditions for extraction process were 60% acetone-water mixture as solvent, solvent/material ratio of 30/1, extraction temperature of 60°C and extraction time of 15 mins. TPC and AC obtained were approximate 43.28±0.54 mg GAE/g DW (Dry weight) and 343.88±3.06 μmol TE/g DW, respectively. The effect of ultrasound-assisted extraction (UAE) on structure of materials of Polygonum multifl orum Thunb. root was observed by scanning electron microscopy (SEM). HPLC method was employed to determinate some main phenolic compounds including catechin, gallic acid, and resveratrol.
References
Aspé, E. and K. Fernández, 2011. The effect of different extraction techniques on extraction yield, total phenolic, and antiradical capacity of extracts from Pinus radiata bark. Ind. Crops Prod., 34: 838-844.
Bajpai, M., A. Pande, S.K. Tewari and D. Prakash, 2005. Phenolic contents and antioxidant activity of some food and medicinal plants. Int. J. Food Sci. Nutr., 56: 287-291.
Benmeziane, F., R. Djamai, Y. Cadot and R. Seridi, 2014. Optimization of extraction parameters of phenolic compounds from Algerian fresh table grapes (Vitis vinifera). Int. Food Res. J., 21: 1061-1065.
Cacace, J.E. and G. Mazza, 2003. Mass transfer processduring extraction of phenolic compounds from milled berries. J. Food Eng., 59: 379-389.
Chemat, S., A. Lagha, H. AitAmar, P.V. Bartels and F. Chemat, 2004. Comparison of conventional and ultrasound-assisted extraction of carvone and limonene from caraway seeds. Flavour Fragrance J., 19: 188-195.
Chirinos, R., H. Rogez, D. Campos, R. Pedreschi and Y. Larondelle, 2007. Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz and Pavón) tubers. Sep. Purif. Technol., 55: 217-225.
Herodež, Š.S., M. Hadolin, M. Škerget and Ž. Knez, 2003. Solvent extraction study of antioxidants from Melissa offi cinalis L. leaves. Food Chem., 80: 275-282.
Hismath, I., W.M. Wan Aida and C.W. Ho, 2011. Optimization of extraction conditions for phenolic compounds from neem (Azadirachta indica) leaves. Int. Food Res. J., 18: 931-939.
Hung, H.C., K.J. Joshipura, R. Jiang, F.B. Hu, D. Hunter, S.A. Smith-Warner, G.A. Colditz, B. Rosner, D. Spiegelman and W.C. Willett, 2004. Fruit and vegetable intake and risk of major chronic disease. J. Natl. Cancer Inst., 96: 1577-1584.
Kim, S.Y., S.M. Jeong, W.P. Park, K.C. Nam, A.U. Ahn and S.C. Lee, 2006. Effect of heating conditions of grape seeds on the antioxidant activity of grape seed extracts. Food Chem., 97: 472-479.
Lapornik, B., M. Prošek and A. GolcWondra, 2005. Comparison of extracts prepared from plant by products using different solvent and extraction time. J. Food Eng., 71: 214-222.
Lin, H.T., S L. Nah, Y Y. Huang and S C. Wu, 2010. Potential antioxidant components and characteristics of fresh Polygonum multifl orum. J. Food Drug Anal., 18: 120-127.
Lin, L., B. Ni, H. Lin, M. Zhang, X. Li, X. Yin, C. Qu and J. Ni, 2015. Traditional usages, botany, phytochemistry, pharmacology and toxicology of Polygonum multifl orum Thunb.: A review. J. Ethnopharmacol., 159: 158-183.
Liu, F.F., C.Y.W. Ang and D. Springer, 2000. Optimization of extraction conditions for active components in Hypericum perforatumusing surface methodology. J. Agric. Food Chem., 48: 3364-3371.
Melecchi, M.I. S., V.F. Péres, C. Dariva, C.A. Zini, F.C. Abad, M.M. Martinez and E.B. Caramã, 2006. Optimization of the sonication extraction method of Hibiscus tiliaceus L. fl owers. Ultrason. Sonochem., 13: 242-250.
Siddiqua, A., K.B. Premakumari, R. Sultana and S. Vithya, 2010. Antioxidant activity and estimation of total phenolic content of Muntingia calabura by colorimetry. Int. J. ChemTech Res., 2: 205-208.
Quoc, L.P.T. and N.V. Muoi, 2015. Alpha-amylase-assisted extraction of polyphenolic compounds from Polygonum multifl orum Thunb. root. J. Sci. Technol., 53: 31-37.
Ruanma, K., L. Shank and G. Chairote, 2010. Phenolic content and antioxidant properties of green chilli paste and its ingredients. Maejo Int. J. Sci. Technol., 4: 193-200.
Shi, J., J. Yu, J. Pohorly, C. Young, M. Bryan and Y. Wu, 2003. Optimization of the extraction of polyphenols from grape seed meal by aqueous ethanol solution. J. Food, Agric. Environ., 1: 42-47.
Soto, C., E. Caballero, E. Pérez and M.E. Zúñiga, 2014. Effect of extraction conditions on total phenolic content and antioxidant capacity of pretreated wild Peumus boldus leaves from Chile.
Food Bioprod. Process., 92: 328-333.
Veggi, P.C., J. Martinez and M.A.A. Meireles, 2013. Fundamentals of microwave extraction. In: F. Chemat and G. Cravotto (Eds.), Microwave-Assisted Extraction for Bioactive Compounds – Theory and Practice, Springer Press, USA, pp. 15-52.
Vinatoru, M., 2001. An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrason. Sonochem., 8: 303-313.
Wang, L. and C.L. Weller, 2006. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Technol., 17: 300-312.
Wang, X.M., L.B. Zhao, T.Z. Han, S.F. Chen and J.L. Wang, 2008. Protective effects of 2,3,5,4’-tetrahydroxystilbene-2-Obeta-D-glucoside, an active component of Polygonum multifl orum Thunb., on experimental colitis in mice. Eur. J. Pharmacol., 578: 339-348.
Wissam, Z., B. Ghada, A. Wassim and K. Warid, 2012. Effective extraction of polyphenols and proanthocyanidins from pomegranate’s peel. Int. J. Pharm. Pharm. Sci., 4: 675-682.
Xu, G., X. Ye, J. Chen and D. Liu, 2007. Effect of heat treatment on the phenolic compound and antioxidantcapacity of citrus peel extract. J. Agric. Food Chem., 55: 330-335.
Zhang, L., Z.C. Tu, T. Yuan, H. Wanga, Z.F. Fu, Q.H. Wena and X.Q. Wang, 2014. Solvent optimization, antioxidant activity, and chemical characterization of extracts from Artemisia Selengnesis Turcz. Ind. Crops Prod., 56: 223-230.
Zhang, Q.A., X. H. Fan, Z.Q. Zhang, T. Li, C.P. Zhu, X.R. Zhang and W. Song, 2013. Extraction, antioxidant capacity and identifi cation of semen astragali complanati (Astragalus complanatus R. Br.) phenolics. Food Chem., 141: 1295-1300.
Zhang, S., R. Chen, H. Wu and C. Wang, 2006. Ginsenoside extraction from Panaxquinquefolium L. (American ginseng) root by using ultrahigh pressure. J. Pharm. Biomed. Anal., 41: 57-63.
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