Evaluation of aflatoxin M1 by ELISA in raw milk in Kosovo during 2016
Keywords:
aflatoxin M1, cow milk, ELISA, KosovoAbstract
Aflatoxins are a class of mycotoxins that are found in agricultural crops produced mainly by fungi Aspergillus flavus and A. parasiticus. When afl atoxin B1 found in the food crops is consumed by cows, metabolizes to aflatoxin M1 (AFM1), which is excreted through milk. Aflatoxin M1 is a human toxin that causes several health problems. In 2016, during the winter and the summer season, 192 raw cow milk samples were collected from small farms in five major regions of Kosovo (Prishtina, Gjilan, Mitrovica, Peja, and Prizren), and analyzed for the aflatoxin M1 level, using Enzyme-Linked Immunosorbent Assay (ELISA). The number of positive samples was 80 (41.7%), from which 43 samples were from the winter season and 37 samples from the summer season. Prizren was the region with the highest number of positive samples for the two seasons examined. The region with the highest percentage of samples that exceeded the maximum tolerable limit for AFM1, according to EU Maximum Tolerable Limit, was Prishtina, with 15.4% of the samples. The difference of AFM1 level between the seasons was statistically significant (P ≤ 0.05 to P ≤ 0.001). The maximum mean concentrations of AFM1 recorded in the winter season were in the range of 0.004-0.109 ng/mL, and during the summer season they are in the range of 0.004-0.833 ng/mL, all coming from the region of Gjilan. The maximum range for this region resulted to be alarming, values that needed to be reevaluated by a confirming method, like HPLC, in another study. During 2016, the occurrence of AFM1 in raw cow’s milk appears to be prevalent in Kosovo.
References
Akiyama, H., Goda, Y., Tanaka, T. & Toyoda, M. (2001). Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-up. Journal of Chromatography A, 932(1-2), 153-157.
Bilandžić, N., Varenina, I. & Solomun, B. (2010). Aflatoxin M1 in raw milk in Croatia. Food Control, 21(9), 1279-1281.
Bilandžić, N., Božić, Đ., Đokić, M., Sedak, M., Kolanović, B. S., Varenina, I. & Cvetnić, Ž. (2014a). Assessment of aflatoxin M1 contamination in the milk of four dairy species in Croatia. Food Control, 43, 18-21.
Bilandžić, N., Božić, Đ., Đokić, M., Sedak, M., Kolanović, B. S., Varenina, I., Tanković, S. & Cvetnić, Ž. (2014b). Seasonal effect on aflatoxin M1 contamination in raw and UHT milk from Croatia. Food control, 40, 260-264.
Chen, R., Ma, F., Li, P. W., Zhang, W., Ding, X. X., Zhang, Q. I., Li, M., Wang, Y.R. & Xu, B. C. (2014). Effect of ozone on aflatoxins detoxification and nutritional quality of peanuts. Food Chemistry, 146, 284-288.
Dimitrieska-Stojković, E., Stojanovska-Dimzoska, B., Ilievska, G., Uzunov, R., Stojković, G., Hajrulai-Musliu, Z. & Jankuloski, D. (2016). Assessment of aflatoxin contamination in raw milk and feed in Macedonia during 2013. Food Control, 59, 201-206.
Hussein, H. S. & Brasel, J. M. (2001). Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology, 167(2), 101-134.
Kos, J., Lević, J., Đuragić, O., Kokić, B. & Miladinović, I. (2014). Occurrence and estimation of aflatoxin M1 exposure in milk in Serbia. Food Control, 38, 41-46.
Liu, Y., Chang, C. C. H., Marsh, G. M. & Wu, F. (2012). Population attributable risk of aflatoxin-related liver cancer: systematic review and meta-analysis. European Journal of Cancer, 48(14), 2125-2136.
Polovinski-Horvatović, M., Jurić, V. & Glamočić, D. (2009). Two year study of incidence of aflatoxin M1 in milk in the region of Serbia. Biotechnology in Animal Husbandry, 25(5-6-2), 713-718.
Rama, A., Latifi, F., Bajraktari, D. & Ramadani, N. (2015). Assessment of aflatoxin M1 levels in pasteurized and UHT milk consumed in Prishtina, Kosovo. Food Control, 57, 351-354.
Rama, A., Montesissa, C., Lucatello, L., Galina, G., Benetti, C. & Bajraktari, D. (2016). A study on the occurrence of aflatoxin M1 in milk consumed in Kosovo during 2009–2010. Food Control, 62, 52-55.
Richard, J. L., Bennett, G. A., Ross, P. F. & Nelson, P. E. (1993). Analysis of naturally occurring mycotoxins in feedstuffs and food. Journal of Animal Science, 71(9), 2563-2574.
Škrbić, B., Živančev, J., Antić, I. & Godula, M. (2014). Levels of aflatoxin M1 in different types of milk collected in Serbia: Assessment of human and animal exposure. Food Control, 40, 113-119.
Unusan, N. (2006). Occurrence of aflatoxin M1 in UHT milk in Turkey. Food and Chemical Toxicology, 44(11), 1897-1900.
Verma, R. J. (2004). Aflatoxin cause DNA damage. International Journal of Human Genetics, 4(4), 231-236.
Bio Scientific, Bio Food and Safety (2013). Max Signal “Aflatoxin M1 ELISA Test Kit.”
European Commission (2006a). Commission regulation 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. Official Journal of the European Union, L 70, 12-34.
European Commission (2006b). Commission regulation 1881/2006 of 19 December2006 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Official Journal of the European Union, L 364, 5-18.
IARC, International Agency for Research on Cancer (1993). Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. In IARC monographs on the evaluation of carcinogenic risk to humans (Vol. 56).
IARC, International Agency for Research on Cancer (2002). Monograph on the evaluation of carcinogenic risk to humans (Vol. 82). Lyon: IARC Press.
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