Indigenous phosphate-solubilizing bacteria enhance germination in deteriorated rice seed
Keywords:
indigenous, phosphate-solubilising bacteria, deteriorated rice seed, germination, 16S rRNA gene, indole, siderophoreAbstract
Certain soil microorganisms have the potential to improve the growth of plants. The aim of this study was to isolate the indigenous bacteria from acid sulphate soil, and evaluate whether this could enhance the germination of deteriorating rice seeds. It was revealed that four bacteria isolates (4A, 6A, 8RH, 10RH) were able to solubilise phosphate and produce indole and siderophore. Molecular identification, using the 16S rRNA gene, showed that these four isolates are Paenibacillus alvei strain NBRC 3343, P. alvei strain DSM 29, Bacillus cereus strain ATCC 14579 and B. cereus strain ATCC 14579. Evaluation showed that the four strains were able to increase the percentage of germination by up to 46.5%, the uniformity of germination by up to 30% and the germination rate by 7.1%/d on seed with ≤ 50% viability. Germination of seeds with 80% viability increased by only 14%, with a slight increase in the uniformity of germination (16%) and germination rate (8.81%/d).
References
Aly, M. M., El-Sabbagh, S., El-Shouny, W. & Ebrahim, M. K. (2001). Salt tolerance of maize under the effect of Azotobacter chroococcum and Streptomyces niveus. J Union Arab Biol, 10, 62-84.
Aly, M. M., El Sayed, H. E. A. & Jastaniah, S. D. (2012). Synergistic effect between Azotobacter vinelandii and Streptomyces sp. isolated from saline soil on seed germination and growth of wheat plant. Journal of American Science, 8(5), 667-676.
Ardakani, S. S., Heydari, A., Tayebi, L. & Mohammadi, M. (2010). Promotion of cotton seedlings growth characteristics by development and use of new bioformulations. International Journal of Botany, 6(2), 95-100.
Bashan, Y., Ream, Y., Levanony, H. & Sade, A. (1989). Nonspecific responses in plant growth, yield, and root colonization of noncereal crop plants to inoculation with Azospirillum brasilense Cd. Canadian Journal of Botany, 67(5), 1317-1324.
Bellenger, J. P., Wichard, T., Kustka, A. B. & Kraepiel, A. M. L. (2008). Uptake of molybdenum and vanadium by a nitrogen-fixing soil bacterium using siderophores. Nature Geoscience, 1(4), 243-246.
Bharathi, R., Vivekananthan, R., Harish, S., Ramanathan, A. & Samiyappan, R. (2004). Rhizobacteria-based bio-formulations for the management of fruit rot infection in chillies. Crop Protection, 23(9), 835-843. https://doi.org/10.1016/j.cropro.2004.01.007
Bhattacharya, A. (2011). Siderophore mediated metal uptake by Pseudomonas fluorescens and its comparison to iron (iii) chelation. Ceylon Journal of Science (Biological Sciences), 39(2), 147-155.
Callan, N. W., Mathre, D. E., Miller, J. B. & Vavrina, C. S. (1997). Biological seed treatments: factors involved in efficacy. HortScience, 32(2), 179-183.
Cattelan, A. J., Hartel, P. G., & Fuhrmann, J. J. (1999). Screening for plant growth–promoting rhizobacteria to promote early soybean growth. Soil Science Society of America Journal, 63(6), 1670-1680.
Chamongkolpradit, W., Budzikiewicz, H., Chanthai, S. & Ruangviriyachai, C. (2008). Immobilized and characteristics of pyoverdin I onto modified micelle-templated silica (MTS) surface as a chelating agent. Asia-Pacific Journal of Science and Technology, 13(2), 182-196.
Chithra, K., Siji, K. & Thomas, B. (2015). Phosphate solubilizing bacteria (PSB) and its priming effect on the germination and growth of Abelmoschus esculentus Linn – A case study. Journal of Science, 5(2), 71-76.
Chookietwattana, K. & Maneewan, K. (2012). Screening of efficient halotolerant phosphate solubilizing bacterium and its effect on promoting plant growth under saline conditions. World Applied Sciences Journal, 16(8), 1110-1117.
del Carmen Jaizme-Vega, M., Rodríguez-Romero, A. S., & Guerra, M. S. P. (2004). Potential use of rhizobacteria from the Bacillus genus to stimulate the plant growth of micropropagated bananas. Fruits, 59(2), 83-90.
Ebrahim, M. K. & Aly, M. M. (2005). Physiological response of wheat to foliar application of zinc and inoculation with some bacterial fertilizers. Journal of plant nutrition, 27(10), 1859-1874.
Egamberdiyeva, D. (2007). The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Applied Soil Ecology, 36(2-3), 184-189.
Govindasamy, V., Senthilkumar, M., Magheshwaran, V., Kumar, U., Bose, P., Sharma, V. & Annapurna, K. (2010). Bacillus and Paenibacillus spp.: potential PGPR for sustainable agriculture. In: Plant growth and health promoting bacteria, Springer, Berlin, Heidelberg, pp. 333-364.
Idris, E. E., Iglesias, D. J., Talon, M. & Borriss, R. (2007). Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amylolique-faciens FZB42. Molecular Plant-Microbe Interactions, 20(6), 619-626.
Joo, G. J., Kim, Y. M., Lee, I. J., Song, K. S. & Rhee, I. K. (2004). Growth promotion of red pepper plug seedlings and the production of gibberellins by Bacillus cereus, Bacillus macroides and Bacillus pumilus. Biotechnology Letters, 26(6), 487-491.
Khan, M. S., Zaidi, A., Wani, P. A. & Oves, M. (2009). Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils. Environmental Chemistry Letters, 7(1), 1-19.
Koumoutsi, A., Chen, X. H., Henne, A., Liesegang, H., Hitzeroth, G., Franke, P., Joachim Vater, J. & Borriss, R. (2004). Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42. Journal of Bacteriology, 186(4), 1084-1096.
Lugtenberg, B. J. J., Chin-A-Woeng, T. F. C. & Bloemberg, G. V. (2002). Microbe-plant interactions: principles and mechanisms. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 81(1-4), 373-383.
Lutts, S., Benincasa, P., Wojtyla, L., Kubala, S., Pace, R., Lechowska, K., Quinet, M. & Garnczarska, M. (2016). Seed priming: new comprehensive approaches for an old empirical technique. In: New Challenges in Seed Biology-Basic and Translational Research Driving Seed Technology. IntechOpen.
McSpadden Gardener, B. B. (2004). Ecology of Bacillus and Paenibacillus spp. in agricultural systems. Phytopathology, 94(11), 1252-1258.
Merzaeva, O. V. & Shirokikh, I. G. (2010). The production of auxins by the endophytic bacteria of winter rye. Applied Biochemistry and Microbiology, 46(1), 44-50.
Meyer, J. M. & Abdallah, M. A. (1980). The siderochromes of non-fluorescent pseudomonads: production of nocardamine by Pseudomonas stutzeri. Microbiology, 118(1), 125-129.
Mrkovacki, N. & Milic, V. (2001). Use of Azotobacter chroococcum as potentially useful in agricultural application. Annals of Microbiology, 51(2), 145-158.
Nadeem, S. M., Ahmad, M., Zahir, Z. A., Javaid, A. & Ashraf, M. (2014). The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnology Advances, 32(2), 429-448.
Nuruddin, A. A., Leng, H. M. & Basaruddin, F. (2006). Peat moisture and water level relationship in a tropical peat swamp forest. Journal of Applied Sciences, 6(11), 2517-2519.
Saitou, N. & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4), 406-425.
Saraswati, R., Husen, E. & Simanungkalit, R. D. M. (2012). Metode analisis biologi tanah. Balai Besar Penelitian dan Pengembangan Sumber Daya Lahan Pertanian. Bogor. Indonesia (ID)
Shaharoona, B., Arshad, M., Zahir, Z. A. & Khalid, A. (2006). Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biology and Biochemistry, 38(9), 2971-2975.
Singh, H., Jassal, R. K., Kang, J. S., Sandhu, S. S., Kang, H. & Grewal, K. (2015). Seed priming techniques in field crops – A review. Agricultural Reviews, 36(4), 251-264.
Sutariati, Gu. A. K. & Ilyas, S. (2006). Effects of plant growth promoting Rhizobacteria on seed germination and seedling growth of hot pepper. Indonesian Journal of Agronomy, 34(1), 46-54.
Thakuria, D., Talukdar, N. C., Goswami, C., Hazarika, S., Boro, R. C. & Khan, M. R. (2004). Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam. Current Science, 86(7), 978-985.
Wahyudi, A. T. (2009). Rhizobacteria Pemacu Pertumbuhan Tanaman: Prospeknya sebagai Agen Biostimulator & Biokontrol. Nano Indonesia.
Wu, S. C., Cao, Z. H., Li, Z. G., Cheung, K. C. & Wong, M. H. (2005). Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma, 125(1-2), 155-166.
Zhao, Q., Shen, Q., Ran, W., Xiao, T., Xu, D. & Xu, Y. (2011). Inoculation of soil by Bacillus subtilis Y-IVI improves plant growth and colonization of the rhizosphere and interior tissues of muskmelon (Cucumis melo L.). Biology and Fertility of Soils, 47(5), 507-514.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 Bulgarian Journal of Agricultural Science
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
