Determining genotypes of 3-breed pig hybrids by marker genes and their interrelation with meat productivity
Keywords:
genotyping, commercial, pig hybrids, slaughter qualities, meat qualities, gene-dependent selection, marker genesAbstract
The paper presents the results of determining genotypes of 3-breed pig hybrids (Landrace × Yorkshire × Duroc) by MC4R, IGF2, POU1F1, H-FABP, GH, LEP marker genes and their interrelation with meat productivity. Allele frequencies for the studied genes have been determined. The studies proved that 3-breed hybrids did not have the highest level of heterozygosity in most allelic genes. There have been identified the most desirable genotypes for the studied genes (GG MC4R, AG MC4R, QQ IGF2, EF POU1F1, DD H-FABP, AA GH, CTLEP), which are recommended for pig selection as well as for selection of parent pairs for producing commercial hybrids with high meat productivity. New methods of estimation and selection along with the traditional ones are used in farm animal breeding. The new methods of animal estimation include modern DNA technologies that allow identifying genes directly or indirectly related to economic traits. By now there have been identified a number of DNA markers associated with economic traits or hereditary and other diseases. However the studies have not been finalized yet and need to be continued to clarify the action of promising gene markers and to search for the new ones that are optimal when used in breeding.
References
Arsiyenko, R.Y. (2003). Polymorphism of protein gene involving fatty acids (H-FABP), and its effect on economic traits of pigs. Dissertation, Podolsk, Russia.
Balatsky, V., Bankovska, I., Pena, R. N., Saienko, A., Buslyk, T., Korinnyi, S., & Doran, O. (2016). Polymorphisms of the porcine cathepsins, growth hormone-releasing hormone and leptin receptor genes and their association with meat quality traits in Ukrainian Large White breed. Molecular Biology Reports, 43(6), 517-526.
Bižienė, R., Miceikienė, I., Baltrėnaitė, L., & Krasnopiorova, N. (2011). Association between growth hormone gene polymorphism and economic traits in pigs. Veterinarija ir Zootechnika, 56(78), 27-31.
Bruun, C. S., Jorgensen, C. B., Nielsen, V. H., Andersson, L., & Fredholm, M. (2006). Evaluation of the porcine melanocortin 4 receptor (MC4R) gene as a positional candidate for a fatness QTL in a cross between Landrace and Hampshire. Animal Genetics, 37(4), 359-362.
Burgos, C., Galve, A., Moreno, C., Altarriba, J., Reina, R., García, C., & López-Buesa, P. (2011). The effects of two alleles of IGF2 on fat content in pig carcasses and pork. Meat Science, 90(2), 309-313.
Chen, J.N., Y.Z. Jiang, W.M. Cen, Xing, S.H., Zhu, L., Tang, G. Q., Li, M. Z., Jiang, A. A., Lou, P. E., Wen, A. X., Wang, Q., He, T., Zhu, G. X., Xie, M. & Li, X. W. (2014). Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations. Genetics and Molecular Research, 13(3), 6759-6772.
Chernukha, I. M., Kovalyova, O. A., Drushlyak, N. G., Radchenko, M. V., Novikova, S. P., & Lisikova, S. A. (2015). Marker breeding potentiality of pigs in economic- and techno-logically valuable traits. Swine Breeding, 4, 14-18.
Chernukha, I. M., Shalimova, O. A., Kryukov, V. I., Druhlyak, N. G., & Radchenko, M. V. (2013). Polymorphism of DNA-markers associated with meat quality in three-breed pig crosses. All about Meat, 2, 30-33.
Clark, D. L., Bohrer, B. M., Tavárez, M. A., Boler, D. D., Beever, J. E., & Dilger, A. C. (2014). Effects of the porcine igf2 intron 3-g3072a mutation on carcass cutability, meat quality, and bacon processing. Journal of Animal Science, 92(12), 5778-5788.
Ernst, L. K., & Zinoviyeva, N. A. (2008). Biological Problems of Livestock Breeding in ХХI Century. L.K. Ernst Federal Science Center for Animal Husbandry, Moscow.
Gardan, D., Gondret, F., Van den Maagdenberg, K., Buys, N., De Smet, S., & Louveau, I. (2008). Lipid metabolism and cellular features of skeletal muscle and subcutaneous adipose tissue in pigs differing in IGF-II genotype. Domestic Animal Endocrinology, 34(1), 45-53.
Getmantseva, L.V. (2012). Influence of MC4R, IGF2 and POU1F1 on production traits of pigs. Dissertation, Rostov Region, Russia.
Getmantseva, L., Kolosov, A., Leonova, M., Usatov, A., Bakoev, F., Klimenko, A., & Bakoev, S. (2017). Effect of polymorphisms in intron 1 of the swine POU1F1 gene on growth and reproductive traits. Turkish Journal of Veterinary and Animal Sciences, 41(5), 643-647.
Getmantseva, L., Leonova, M., Usatov, A., Bakoev, S., Klimenko, A., Vasilenko, V., Kolosov, A., Shirockova, N., & Makarenko, M. (2014). The single and combined effect of MC4R and GH genes on productive traits of pigs. American Journal of Agricultural and Biological Sciences, 12(1), 28-32.
Gilbert, H., & Le Roy, P. (2007). Methods for the detection of multiple linked QTL applied to a mixture of full and half sib families. Genetics Selection Evolution, 39(2), 139-158.
Goncharenko, G., & Grishina, N. (2010). Production traits of pigs evaluated through the use of immunogenetic and DNA-markers. Chief Zoo Engineer, 3, 10-13.
Hager, R., Cheverud, J. M., & Wolf, J. B. (2008). Maternal effects as the cause of parent–of–origin effects that mimic genomic imprinting. Genetics, 178(3), 1167-1175.
Houston, R. D., Haley, C. S., Archibald, A. L., & Rance, K. A. (2005). A QTL affecting daily feed in take maps to Chromosome 2 in pigs. Mammalian Genome, 16, 464-470.
Jirtle, R. L. (2004). IGF2 loss of imprinting: a potential heritable risk factor for colorectal cancer. Gastroenterology, 126, 1190-1193.
Kabanov, V. D. (2001). Hereditary homogenization and its role in animal breeding. Herald of the Russia Academy of Agrarian Sciences, 5, 59-61.
Karpushkina, T. V., Kostyunina, O. V., Sizaryova, E. I., & Zinoviyeva, N. A. (2016). Production traits of LW pigs related IGF2 and CTSD genotype. Achievements of Science and Technology of AIC, 30(6), 82-85.
Kim, G.-W., Yoo, J.-Y., & Kim, H.-Y. (2014). Association of genotype of POU1F1 intron 1 with carcass characteristics in crossbred pigs. Journal of Animal Science and Technology, 56, 25.
Kim, K. S., Lee, J. J., Shin, H. Y., Choi, B. H., Lee, C.-H., Kim, J.-J., Cho, B. W., & Kim, T.-H. (2006). Association of melanocortin 4 receptor (MC4R) and high mobility group AT-hook 1 (HMGA1) polymorphisms with pig growth and fat deposition traits. Animal Genetics, 37(4), 419-421.
Korwin-Kossakowska, A., Sender, G., & Kurył, J. (2004). Associations between the microsatellite DNA sequence in the IGF1 gene, polymorphism in the ESR gene and selected reproduction traits in F1 (Zlotnicka Spotted × Polish Large White) sows. Animal Science Papers and Reports, 22(2), 215-226.
Kostyunina, O. V., Kramarenko, C. C., & Svezhentseva, N. A. (2015). IGF2 gene association with production traits of LW pigs (Sus scrofa) with regard to sex differentiation. Agricultural Biology, 50(6), 736-745.
Kostyunina, O. V., Levitchenko, A. N., & Zinoviyeva, N. A. (2008). IGF2 gene is prospective DNA-marker of meat and fattening pig production. Russia Livestock Breeding, 1, 12-14.
Kostyunina, O. V., Svezhentseva, N. A., Zinoviyeva, N. A., Dotsev, A. V., Shahin, A. V., Sizareva, E. I., & Gladyr, E. A. (2011). Influence of marker ESR and IGF2 genotype on breeding value of LW boars. Agricultural Biology, 6, 54-59.
Kostyunina, O. V., Zinoviyeva, N., Levitchenko, A., & Gogolev, A. (2009). Breeding based on DNA-technologies. Swine Breeding, Special issue, 19-20.
Kostyunina, O. V., Zinoviyeva, N. A., Sizaryova, E. I., Ka lugina, A. I., Gladyr, E. A., Getmantseva, L. V., Fornara, M. S., & Kharzinova, V. R. (2012). Polymorphism of melanocortin receptor gene MC4R and their effect on the growth and meat productive traits of pigs. Achievements of Science and Technology of AIC, 8, 49-51.
Lee, S. S., Chem, Y., Moran, C., Cepica, S., Reiner, G., Bartenschlager, H., Moser, G., & Geldermann, H. (2003). Linkage and QTL mapping for Sus scrofa chromosome 2. Journal of Animal Breeding and Genetics, 120, 11-19.
Liu, D., Hu, Y., Yang, X., Liu, Y., Wei, S., & Jiang, Y. (2011). Identification and genetic effects of a novel polymorphism in the distal promoter region of porcine leptin gene. Molecular Biology Reports, 38(3), 2051-2057.
Loban, N. A. (2010a). Production level and new methods of improving Belorussian Large White pigs. In: Modern Problems of Intensification of Pork Production in CIS Countries, Collection of scientific papers of 17th International Scientific and Production Conference on Swine Breeding dedicated to the 75th anniversary of Honored Scientist of the Russian Federation, Professor V.E. Ulitko, 7-10 July, 2010, Ulyanovsk, Russia, 221-226.
Loban, N., (2010b). Yorkshires in line breeding and production. Russia Livestock Breeding, 2, 26-28.
Loban, N., Vasilyuk, O., & Chernov, A. (2004). Polymorphism type effect of H-FABP gene on some production traits of pigs. Swine Breeding, 5, 8-9.
Lyadski, I. K., Getya, A. A., & Pochernyaev, K. F. (2011). Correlation between the Asp298asn-polymorphism of MC4R gene and back fat width in large white pigs. Cytology and Genetics, 45(2), 52-56.
Maksimov, G. V., & Getmantseva, L. V. (2011). Influence of MC4R gene on meat production of pigs. Chief Zoo Engineer, 10, 9-12.
Maksimov, G., Maksimov, A., & Lenkova, N. (2015). DNA-genotyping on RYRI, ESR and H-FABP alleles and meat production of pigs. Chief Zoo Engineer, 1, 24-31.
Octura, J. E. R., Yambao, R. R. C. S., Santiago, R. C., Cho, B.-W. & Vega, R. S. A. (2014). Polymorphism in the Melanocortin-4 Receptor (MC4R) gene and its effect on fatness and weight performance of Philippine Native Pigs: A preliminary study. International Journal of Sciences: Basic and Applied Research, 15(1), 464-474.
Pang, W. J., Bai, L., & Yang, G. S. (2006). Relationship among H-FABP gene polymorphism, intramuscular fat content, and adipocyte lipid droplet content in main pig breeds with different genotypes in western China. Acta Genetica Sinica, 33(6), 515-524.
Peixoto, J. de O., Faria, D. A. de, Silva, P. V., Fonseca, I., Lopes, P. S., & Guimarães, S. E. F. (2009). Association between leptin gene single nucleotide polymorphisms and carcass traits in pigs. Revista Brasileira de Zootecnia, 38(2), 271-276.
Pérez-Montarelo, D., Fernández, A., Barragán, C., Noguera, J. L., Folch, J. M., Rodríguez, C., Óvilo, C., Silió, L., & Fernández, A. I. (2013). Transcriptional characterization of porcine leptin and leptin receptor genes. Plos One, 8(6).
Pérez-Montarelo, D., Fernández, A., Folch, J. M., Pena, R. N., Ovilo, C., Rodríguez, C., Silió, L., & Fernández, A. I. (2012). Joint effect of porcine leptin and leptin receptor polymorphisms on productivity and quality traits. Animal Genetics, 43(6) 805-809.
Petukhov, V. L., Korotkevich, O. S., Stambekov, S. Zh., Zhigachev, A. I., & Bakai, A. V. (2007). Genetics. Semipalatinsk State Pedagogical Institute, Novosibirsk.
Polozyuk, O. N., Maksimov, G. V., Getmantseva, L. V., & Bublik, E. M. (2014). Gene markers influencing production of pigs. Swine Breeding, 4, 35-36.
Raskatova, S. M., Shavirina, K. M., & Zinoviyeva, N. A. (2015). Studying meat and fattening traits on the basis of polymorphism in IGF2, MC4R, CAST, PRKAG3 and CCKAR genes in pig youngsters. Swine Breeding, 1, 25-28.
Sandor, C., & Georges, M. (2008). On the detection of imprinted quantitative trait loci in line crosses: effects of linkage disequilibrium. Genetics, 178, 1755-1762.
Sayenko, A. M., & Balatski, V. N. (2009). Polymorphism of RYRI, ESR, PRLR and GH in pig populations of various breeds. In: Materials of the 18th Meeting of the Interuniversity Coordinaion Council on Swine Breeding and International Scientific and Production Conference “Current Problems of Pork Production in the Russian Federation”, 2-4 June, 2009, Sochi, Russia, 74-77.
Semyonov, V. V., Chizhova, L. N., Pluzhnikova, O. V., Marutyants, N. G., Lozovoy, V. I., & Serdyukov, E. I. (2013). Using RYR-1, ESR and H-FABP gene polymorphism in swine breeding. Herald of the Russia Academy of Agrarian Sciences, 2, 65-67.
Tyra, M., Ropka-Molik, K., Eckert, R., Piórkowska, K., & Oczkowicz, M. (2010). H-FABP and LEPR gene expression profile in skeletal muscles and liver during ontogenesis in various breeds of pigs. Domestic Animal Endocrinology, 40(3), 147-154.
Uemoto, Y., Kikuchi, T., Nakano, H., Sato, S., Shibata, T., Kadowaki, H., Katoh, K., Kobayashi, E., & Suzuki, K. (2012). Effects of porcine leptin receptor gene polymorphisms on back-fat thickness, fat area ratios by image analysis, and serum leptin concentrations in a Duroc purebred population. Animal Science Journal, 83(5), 375-385.
Van Den Broeke, A., Aluwe, M., Tuyttens, F. A., Ampe, B., Van-haecke, L., Wauters, J., Janssens, S., Cousse, A., Buys, N., & Millet, S. (2015). An invention study demonstrates effects of MC4R genotype on boar taint and performances of growing-finishing pigs. Journal of Animal Science, 93(3), 934-943.
Van Den Maagdenberg, K., Claeys, E., Stinckens, A., Buys, N., & Smet, S. De (2007). Effect of age, muscle type, and insulin–like growth factor–II genotype on muscle proteolytic and lipolytic enzyme activities in boars. Journal of Animal Science, 85(4), 952-960.
Van Laere, A.-S., Nguyen, M., Braunschweig, M. H., Carine, N., Collette, C., Moreau, L., Archibald, A. L., Haley, C., Buys, N., Tally, M., Andersson, G., Georges, M., & Andersson, L. (2003). A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature, 425(6960), 832-836.
Zinoviyeva, N. A. (2010). DNA-technologies in swine-breeding. Chief Zoo Engineer, 5, 12-14.
Zinoviyeva, N. A., & Glad ir, E. A. (2011). DNA-technologies in cell engineering and marking farm animal traits. Achievements of Science and Technology of AIC, 9, 3-7.
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