Induce resistance against bacterial speck disease of tomato Pseudomonas syringae pv. tomato by the chemical inducer β-aminobutyric acid
Keywords:
induce resistance, Pseudomonas syringae pv. Tomato, β-aminobutyric acidAbstract
The ability of ß-aminobutyric acid to induce resistance tomato (Lycopersicon esculentum Mill. var. GS12) seedlings against bacterial speck disease was studied. Different concentrations were used as: 100, 200, 300, 400, 500, 600 mg/lafter tomato plants were sprayed with 108CFU/ml foliar Pseudomonas syringae pv. tomato suspension. The results revealed that the concentration of 100 mg/l of ß-aminobutyric acid was the most efficient in reducing disease incidence and severity, furthermore it was found that all used concentrations improved tomato seedlings growth compared with control. The response of the treated tomato seedlings to the different concentration of ß-aminobutyric acid to induce systemic acquired resistance against bacterial speck (P. syringae pv. tomato) was found to be correlated to fluctuations with the amount of some compounds known to interfere with the defense mechanism against pathogen invading including: free salicylic acid, ethylene, chitinase, peroxidase, ß-1,3- glucanase enzymes, protein content, and total phenolic compounds. The biochemical analysis indicated that tomato seedlings sprayed with 100 mg/l of ß-aminobutyric acid produced highest amounts of ethylene 9 and 15 days post inoculation which was significantly different from control, while highly recovered free salicylic acid was 12 days post inoculation. The recovered level of endogenous enzymes peroxidase and ß-1,3- glucanase was increased over time post inoculation, where the highest amount was 15 days post inoculation. The highest recovered chitinase was 9 days post inoculation and protein compounds contents were increased gradually over time and the highest amount were 12 days post inoculation, in which, it was found that was significantly different compared with control. These results were correlated with low level of disease incidence and severity compared with control and with the high recovered population of P. syringae pv. tomato of 5.38*108CFU /ml after 21 days of inoculation. The results revealed that there is no correlation between total phenolic contents in tomato seedlings treated with ß-aminobutyric acid on induces systemic resistance.
References
Agrios, G. N. (2005). Plant pathology. 5th Edition, Elsevier Academic Press, New York, 922.
Baccelli, I. & Mauch-Mani, B. (2016). Beta-aminobutyric acid priming of plant defense: the role of ABA and other hormones. Plant Molecular Biology, 91(6), 703–711.
Becot, S., Pajot, E., LeCorr, D., Monot, C. & Silue, D. (2000). Phytogard (K2HP03) induces localized resistance in cauliflower to downy mildew crucifers. Crop Protection, 19, 417-425.
Benhamou, N. & Belangaer, R. R. (1998). Benzothiadiazole -mediated induce resistance to Fusarium oxysporium f.sp. Iycopersici in tomato. Plant Physiol., 118, 1203-1212.
Brisset, M., Cesbron, S., Thomoson, S. V. & Paulin, J. (2000). Acibenzolar-S-methyl induces the accumulation of defense- related enzymes in apple and protects from fire blight. European Journal of Plant Pathology, 106, 529-536.
Brudbary, J. F. (1986). Guide plant pathogenic bacteria. CAB International Center, 71 -81.
Ckmus, C. & Sayar, A. H. (1991). Effect of salicylic acid on the control of bacterial speck of tomato caused by Pseudomonas syringae pv. tomate. Journal of Turkish Phytopathology, 20(1), 27- 32.
Cohen, Y. R. (2002). β-Aminobutyric Acid-Induced Resistance Against Plant Pathogens. Plant Disease, 86(5), 448-58.
Colin, J. E. & Chafik, Z. (1986). Comparison of biological and chemical treatments for control of bacterial speck of tomato under field conditions in Morocco. Plant Disease, 70, l1048-1050.
Department of Statistics (DOS) (2016). Retrived from: http://www.dos.gov.io/a~r/agr a/index.htm
Duffy, B. K. & Defago, G. (1999). Macro-and microelement fertilizers influence the severity of Fusarium crown and root rot of tomato in soilless production system. Hort. Science, 34 (2), 287-291.
Enyedi, A. J., Yalpani, N., Silverman, P. & Raskin, I. (1992). Localization, conjugation, and function of salicylic acid in tobacco during the hypersensitive reaction to tobacco mosaic virus. Proc Natl. Acad. Sci., USA, 89, 2480-2484.
Friedrich, L., Lawton, K., Ruess, W., Masner, P., Specker, N., Rella, M. G., Meier, B., Dincher, S., Satub, T., Uknes, S., Merraux, J., Kessmann, H. & Ryals, J. (1996). A benzothiadiazole derivative induces systemic acquired resistance in tobacco. The Plant Journal, 10 (1), 61-70.
Godard, J., Ziadi, S., Monoto, C., LeCorre, D. & Silue, D. (1999). Benzothiadiazole (BTH) induces resistance in cauliflower (Barasica oleracea var. botrytis) to downy mildew of crucifers caused by Peronospora parasitica. Crop Protection, 18, 397-405.
Good, M. I. & Sasser, M. (1980). Prevention the key to controlling bacterial spot and bacterial speck of tomato. Plant Disease, 64, 83 1-834.
Grenier, J. & Asselin, A. (1990). Some pathogenesis-related proteins are chitosanases with lytic activity against fungal spores. Molecular Plant- Microbe Interaction, 3 (6), 401-407.
Herman, M. A. B. & Smart, C. D. (2008). An integrated management strategy for bacterial speck disease of tomato in New York: Deciphering the web of induced resistance. Ph. D. Dissertation, Graduate School of Cornell University, USA.
Hoffland, E., Bakker, A. H. M. & Van Loon, L. C. (1997). Multiple disease protection by rhizobacteria that induce systemic resistance. Letter to the editor. Phytopathology, 138.
InKringe N. R. & Holt, J. G. (1984). (Editor). Bergeys manual of systemic bacteriology, 1, Williams and Wilkins, 950.
Jakab, G., Cottier, V., Toquin, V., Rigoli, G., Zimmerli, L., M´etraux, J. & Mauch-Mani, B. (2001). β-aminobutyric acid-induced resistance in plants. European Journal of Plant Pathology, 107 (1), 29-37.
Johnson, J. & Schaall, L. A. (1957). Chlorogenic acid and ortho o-dihydric phenols in Scat. Susceptibility triumph potato tubers of different maturates. Phytopathology, 47, 253-255.
Junjun, I., Zingen-Sell, Z. & Buchenuer, H. (1996). Induction of resistance of cotton plants to verticillium wilt and of tomato plants to fusarium wilt by 3-aminobutyric acid and methyl jasmonate. Journal of Plant Diseases and Protection, 103(3), 288-299.
Khlaif, H. M. (1991). Bacterial spot of tomato in Jordan: Identification and response of some tomato cultivars to the causal agent. Mutah Journal for Research and Studies, 6, 197-207.
Kim, Y. C., Kim, Y. H., Lee, Y. H., Lee, S. W., Chae, Y. S., Kang, H. K., Yun, B. W. & Hong, J. K. (2013). β-Amino-n-butyric acid regulates seedling growth and disease resistance of Kimchi Cabbage. Plant Pathology Journal, 29(3), 305-316.
Knoester, M., Pieterse, C. M. J., Bol, J. F. & Van Loon, L. C. (1999). Systemic resistance in Arabidopsis induced by rhizobacteria required ethylene-dependent signaling at the site of application. Molecular Plant-Microbe Interaction, 12(8), 720-727.
Leon, J., Shulaev, V., Yalpani, N., Lawto, M. A. & Raski, I. (1995). Benzoic acid 2-hydroxylase, a soluble oxygenase from tobacco, catalyzes salicylic acid biosythesis. Proc. Natl. Acad. Sci., USA 92, 10413-10417.
Li, W., Liu, J., Ashraf, U., Li, G., Li, Y., Lu, W., Gao, L., Han, F. & Hu, J. (2016). Exogenous γ-aminobutyric acid (GABA) application improved early growth, net photosynthesis, and associated physio-biochemical events in maize. Frontiers in Plant Sciences, 7 (919).
Lowy, O. H., Rosebrough, N. J., Farr, A. L. & Randdall, R. J. (1951). Protein measurement with foline phenol reagent. Journal of Biochemistry, 193, 265-275.
Lund, T. S., Stall, R. E. & Klee, H. J. (1998). Ethylene regulates the susceptible response to pathogen infection in tomato. The Plant Cell, 10: 371-382.
Orober, M., Siegrist, J. & Buchcnauer, M. (1996). Induction of systemic acquired resistance in cucumber by foliar application. 339-348. In: Modern fungicides and antifingal compounds, Intercept Itd. London.
Penninckx, A. M., Thomma, B. P. H., Buchala, A., Metraux, J. & Broekaert, V. F. (1998). Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis. Plant Cell, 10, 2103-2114.
Pernezy, K., Kudela, V., Kokoskova, B. & Hladka, I. (1995). Bacterial diseases of tomato in the Czech and Slovak Repiblics and lack of sterpomycin resistance among copper-tolerant bacterial strains. Crop Protection, 14(4), 267-270.
Pilowsky, M. & Zutra, D. (1982). Screening wild tomatoes for resistance to bacterial speck pathogen Pseudomonas syringae pv. tomato. Plant Disease, 66, 46- 47.
Rajaei, P. & Mohamadi, N. (2013). Effect of beta aminobutyric acid (BABA), ABA and ethylene synthesis inhibitor (CoCl2) on seed germination and seedling growth of Brassica napus L. European Journal of Experimental Biology, 3(5), 437-440.
Ramesh, S. A., Tyerman, S. D., Xu, B., Bos, J., Kaur, S., Conn, V., Domingos, P., Ullah, S., Wege, S., Shabala, S., Feijo, J. A., Ryan, P. R. & Gillham, M. (2015). GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters. Nature Communications, 6:7879. DOI:10.1038/ncomms8879|www.nature.com/naturecommunications
Saskia, C. M., Corne, P., Annemiek, T., Yronne, V. A., Femke, H. & Leendert, L. C. (1997). Differential induction of systemic resistance in Arabidopsis by bio control bacteria. Molecular Plant Microbe Interactions, 10 (6), 7 16-724.
Sathiyabama, M. & Balasubramanian, R. (1999). Treatment of groundnut leaves with salicylic acid controls the development of rust disease caused by Puccinia arachidis Speg. Journal of Plant Diseases and Protection, 106 (2), 166-173.
Schaad, N. W., Jones, J. B. & Chun, W. (2001). Laboratory guide for identification of plant pathogenic bacteria, 3d edition. APS Press, USA.
Siegrist, J. D., KolIe, G. C. & Schmitk, M. (1997). Chemically induced resistance in green bean against bacterial and fungal pathogens. Journal of Plant Disease and Protection, 104(6), 599-610.
Smith-Becker, J., Marosis, E. & Keen, N. T. (1998). Accumulation of salicylic acid and 4-hydroxybenzoic acid in phloem fluids of cucumber during systemic acquired resistance is preceded by a transient increase I phenylalnine ammonia lyase activity in petioles and stem. Plant Physiology, 1(16), 213-238.
Spletzer, M. E. & Enyedia, A. J. (1999). Salicylic acid induces resistance to Alternariasolaniin hydrobonic grown tomato. Phytopathology, 89(9), 722-727.
Tamm, L., Thurig, B., Fliessbach, A., Goltlieb, A. E., Karavani, S. & Cohen, Y. (2011). Elicitors and soil management to induce resistance against fungal plant diseases. Wageninggen Journal of Life Sciences, 58, 131-137.
Thevenet, D., Pastor, V., Baccelli, I., Balmer, A., Vallat, A., Neier, R., Glauser, G. & Mauch-Mani, B. (2017). The priming molecule b-aminobutyric acid is naturally present in plants and is induced by stress. New Phytologist, 213, 552–559.
Van Loon, L. C., P. A. H. M. Bakker and C. M. J. Pieterse, 1998. Systemic resistance induced by rhizosphere bacterial. Annu. Rev. Phytopathology, 36, 453-483.
Wilkinsona, S. W., Pastorb, V., Paplauskasa, S., Petriacqac, P. & Lunaa, E. (2018). Long-lasting b-aminobutyric acid-induced resistance protects tomato fruit against Botrytis cinerea. Plant Pathology, 67, 30–41.
Yoo, S., Shina, D. J., Wona, H. Y., Songa, J. & Sanga, M. K. (2018). Aspergillus terreus JF27 promotes the growth of tomato plants and induces resistance against Pseudomonas syringae pv. tomato. Mycobiology, 46 (2), 147–153.
Yunis, H., Bashan, Y., Okon, Y. & Henis, Y. (1980). Weather dependence yield losses and control of bacterial speck of tomato caused by Pseudomonas syringae pv. tomato. Plant Disease, 64, 937-939.
Zimmerli, L., Jakals, G., Metraux, J. & Brigitte, M. (2000). Potentation of pathogen-specific defense mechanisms in Arabidopsis by Caminobutyric acid. Proc. Natl. Acad. Sci. USA, 97 (23), 12920-12925.
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