Influence of IMMUNOSTART HERB feeding on the bee colonies development

Authors

  • Rositsa Shumkova Agricultural Academy, Research Center of Stockbreeding and Agriculture, Smolyan, Bulgaria Author
  • Ralitsa Balkanska Agricultural Academy, Institute of Animal Science, Kostinbrod, Bulgaria Author

Keywords:

bee colony development, bee feeding, hemolymph, hypopharyngeal glands, IMMUNOSTART HERB

Abstract

To date, various plant-based supplemental forages, which contain key molecules, for bees are being used worldwide to improve the bee health. The aim of the study was to determine the effect of the product IMMUNOSTART HERB on some physiological and biochemical parameters and the development of the bee colonies during their spring feeding. The study was conducted at the experimental apiary of the Research Center of Stockbreeding and Agriculture, Smolyan, Bulgaria. The experiment included local honey bees Apis mellifera L., settled in the Langstroth-Rut system. For the feeding of the bee colonies is used a natural product IMMUNOSTART HERB based on herbal extracts. It contains natural flavonoids, polyphenols, polysaccharides, mucus substances, amino acids, essential oils, vitamins, minerals and vitamin C. The feeding of the bee colonies in the spring was carried out as follows: the experimental group was fed with IMMUNOSTART HERB at a dose of 10 ml in 100 ml sugar solution (sugar/water 1:1) 4 times at intervals of 7 days; the control group received only sugar solution. Statistically significant differences were found for the strength of the bee colonies and the amount of sealed worker bee brood in the experimental group fed with IMMUNOSTART HERB compared to the control group. A linear positive correlation between the fresh bee head weight and the degree of development of the hypopharyngeal glands was found. The higher values of total protein in the haemolymph in the experimental group were found to be indicative of the positive effect of the product IMMUNOSTART HERB on the immunity of bees. According to this study, the supplementary feeding of the bee colonies with IMMUNOSTART HERB is good to start about 60 days before the first main pasture in the region.

References

Alaux, C., Ducloz, F., Crauser, D. & Le Conte, Y. (2010). Diet effects on honeybee immunocompetence. Biology Letters, 6, 562-565.

Al-Ghamdi, A.A., Al-Khaibari, A.M. & Omar, M.O. (2011). Consumption rate of some proteinic diets affecting hypopharyngeal glands development in honeybee workers. Saudi Journal of Biological Sciences, 18, 73-77.

Andi, M. A. & Ahmadi, A. (2014). Influence of vitamin C in sugar syrup on brood area, colony population, body weight and protein in honey bees. International Journal of Biosciences, 4(6), 32-36.

Basualdo, M., Barragan, S. & Antunez, K. (2014). Bee bread increases honeybee haemolymph protein and promote better survival despite of causing higher Nosema ceranae abundance in honeybees. Environmental Microbiology Reports, 6, 396-400.

Branchiccela, B., Castelli, L., Corona, M., Díaz-Cetti, S., Invernizzi, C., Martínez de la Escalera, G., Mendoza, Y., Santos, E., Silva, C., Zunino, P. & Antúnez, K. (2019). Impact of nutritional stress on the honeybee colony health. Scientific Reports, 9, 1-11.

Crailsheim, K. (1990). The protein balance of the honey bee worker. Apidologie, 21, 417-429.

DeGrandi-Hoffman, G., Chen, Y., Huang, E. & Huang, M.H. (2010). The effect of diet on protein concentration, hypopharyngeal gland development and virus load in worker honey bees (Apis mellifera L.). Journal of Insect Physiology, 56, 1184- 1191.

Di Pasquale, G., Marion, S., Le Conte, Y., Belzunces, L., Decourtye, A., Kretzschmar, A., Suchail, S., Brunet, J.-L. & Alaux, C. (2013). Influence of pollen nutrition on honey bee health: Do pollen quality and diversity matter? PLoS One, 8, 1-13.

Genersch, E. (2010). Honey bee pathology: Current threats to honey bees and beekeeping. Applied Microbiology and Biotechnology, 87(1), 87-97.

Gurgulova, K., Valchovski, R., Zhelyazkova, I. & Nenchev, P. (2001). Effect of some stimulating substances on natural immunity on honey bees Apis mellifera. Zhivotnovadni Nauki, 2, 113-115 (Bg).

Hristakov, I. (2012). Research on the stimulating effect of Tribulus terrestris on the oviparous activity of the queen bees. Agricultural Science and Technology, 4(3), 228-233.

Hristakov, I., Zhelyazkova, I. & Hvarchilkov, V. (2013). Researching the resistance of bees fattened up with additive of extract of Tribulus terrestris L. during wintering. Agricultural Science and Technology, 5 (1), 75-78.

Huang, Z. & Otis, G.W. (1989). Factors determining hypopharyngeal gland activity of worker honey bees (Apis mellifera L.). Insectes Sociaux, 36, 264-276.

Klose, S. P., Rolke, D. & Baumann, O. (2017). Morphogenesis of honeybee hypopharyngeal gland during pupal development. Frontiers in Zoology, 14(22), 1-14.

Kunc, M., Dobeš, P., Hurychová, J., Vojtek, L., Beani Poiani, S., Danihlík, J., Havlík, J.,Titera, D. & Hyršl, P. (2019). The year of the honey bee (Apis mellifera L.) with respect to its physiology and immunity: A search for biochemical markers of longevity. Insects, 10, 1-15.

Lee, M., Choi, Y., Kim, D. & Lee, M. (2019). Age-dependent hypopharyngeal gland development and morphometric characteristics in the cross-bred lineage of honeybees reared for high royal jelly production. Journal of Asia-Pacific Entomology, 22(3), 699-704.

Maurizio, A. (1954). Pollenernarung und Lebensvorgange bei der Honigbiene (Apis mellifera). Landwirt Jahrbuch Schweiz, 68, 115-182.

Negri, P., Villalobos, E., Szawarski, N., Damiani, N., Gende, L., Garrido, M., Maggi, M., Quintana, S., Lamattina, L. & Eguaras, M. (2019). Towards precision nutrition: A novel concept linking phytochemicals, immune response and honey bee health. Insects, 10, 1-29.

Neov, B., Georgieva, A., Shumkova, R., Radoslavov, G. & Hristov, P. (2019). Biotic and abiotic factors associated with colonies mortalities of managed honey bee (Apis mellifera). Diversity, 11(237), 1-16. doi: 10.3390/d11120237

Pătruică, S., Bănăţean-Dunea, I., Jivan, A., Jivan, A. & Stroe, A. (2011). Research on the influence of apiary biostimulators on bee families development in spring. Scientific Papers: Animal Science and Biotechnologies, 44(2), 267-270.

Ratnieks, F.L. & Carreck, N.L. (2010). Clarity on honey bee collapse? Science, 327(5962), 152-153.

Shumkova, R. (2016). Comparative study on the effect of stimulating products on the biological development of bee colonies. PhD Thesis, Institute of Animal Science, Kostinbrod, Bulgaria (Bg).

Škerl, M.I. & Gregorc, A. (2015). Characteristics of hypopharyngeal glands in honeybees (Apis mellifera carnica) from a nurse colony. Slovenian Veterinary Research, 52(2), 67-74.

Wu, J.Y., Anelli, C.M. & Sheppard, W.S. (2011). Sub-lethal effects of pesticide residues in brood comb on worker honey bee (Apis mellifera) development and longevity. PLoS One, 6(2), e14720.

Zahra, A. & Talal, M. (2008). Impact of pollen supplements and vitamins on the development of hypopharyngeal glands and on brood area in honey bees. Journal of Apicultural Sciences, 52(2), 5-11.

Zhelyazkova, I., Gurgulova, K., Malinova, K. & Ali, A.S. (2009). Study on the stimulating effect of the herbal preparations „Green TM“ and „Ecophil-P“ on the development of the bee colonies. Proceedings of International Scientific Conference, 4-5. VІ. 2009, Stara Zagora, 148-153 (Bg).

Zhelyazkova, I., Gurgulova, K., Panchev, I. & Popova, V. (2008). Influence of “Startovit”, “Apitonus” and “Apisaniran” on the bee colony productivity parameters. Beekeeping – simple and clear. Bucharest, Romania, 11-14.IX. 2008, Book of abstracts, 41-44.

Zhelyazkova, I., Gurgulova, K., Panchev, I. & Popova, V. (2010). Effect of the biological stimulating product Apisaniran on the productive parameters of bee colonies. Apimondia First World Conference on Organic Beekeeping, 27-29 August 2010, Sunny Beach, Bulgaria, Book of abstracts, 82.

Downloads

Published

31.10.2021

How to Cite

Shumkova, R., & Balkanska, R. (2021). Influence of IMMUNOSTART HERB feeding on the bee colonies development. Bulgarian Journal of Agricultural Science, 27(5), 896-902. https://agriacad.eu/ojs/index.php/bjas/article/view/2126