Anesthetic effect of white camphor essential oil (Cinnamomum camphora L. 1753) and its use for transport of bighead carp (Hypophthalmichthys nobilis R. 1845) stocking material
Keywords:
Hypophthalmichthys nobilis, bighead carp, Cinnamomum camphora, white camphor oil, transport, anesthesiaAbstract
The aim of the present study is to examine the efficacy of white camphor essential oil as an anesthetic for bighead carp (Hypophthalmichthys nobilis R. 1845) and as an sedative agent for transport of bighead carp stocking material. The fish used in the research have an average body weight (BW, g) of 35.02±16.58 g and an average total length (TL, cm) of 15.44±2.33 cm. For the experiment of the anesthetic effect of C. camphora, five treatments are conducted with five experimental concentrations: 0.20 ml.l-1, 0.30 ml.l-1, 0.40 ml.l-1, 0.50 ml.l-1 and 0.60 ml.l-1. For each concentration 10 individuals are used or a total of 50 fish. For the transport experiment, 3 concentrations are used: 0.06 ml.l-1, 0.08 ml.l-1 and 0.10 ml.l-1. The applied stocking density is 3 fish/l. For each concentration 30 fish are used or a total of 90 fish. Based on the results it can be concluded that at concentration of 0.60 ml.l-1 the induction of anesthesia is the fastest (4.88±0.43 min), thus the recovery time at this concentration is the longest (9.44±1.28 min). From all tested concentrations the recovery time is the shortest at the lowest concentration (0.20 ml/l) – 2.29±0.32 min (P ≤ 0.001). The behavior of the fish and the phases in which they enter (Phase 0 and Phase 2) change every hour in all experimental variants of the transport experiment. For transport of bighead carp stocking material it can be recommended to apply for 1 h a concentration of 0.10 ml.l-1, at which the highest percentage of fish are in state of decreased locomotor activity and static position. At the lowest concentration of 0.06 ml.l-1 the highest percentage of bighead carp stocking material remain in active state throughout the experiment, which makes this concentration of C. camphora inapplicable for transport of H. nobilis.
References
Akbary, P., Pirbeigi, A. & Jahanbakhshi, A. (2016). Analysis of primary and secondary stress responses in bighead carp (Hypophthalmichthys nobilis) by anesthetization with 2-phenoxyethanol. International Journal of Environmental Scienceand Technology, 13(1), 1009-1016.
Aydın, B. & Barbas, L. (2020). Sedative and anesthetic properties of essential oils and their active compounds in fish: A review. Aquaculture, 520(1), 1-9.
Azad, I. S., Al-Yaqout, A. & Al-Roumi, M. (2014). Antibacterial and immunity enhancement properties of anaesthetic doses of thyme (Thymus vulgaris) oil and three other anaesthetics in Sparidentex hasta and Acanthopagrus latus. Journal of King Saud University-Science, 26(2), 101-106.
Balamurugan, J., Kumar, T., Sanjeevi, P., Bharathiamma, M., Balasundaram, C. & Harikrishan, R. (2016). Clove extract: A potential source of stress free transport of fish. Aquaculture, 454(1), 171-175.
Benovit, S., Gressler, L. & Silva, L. (2012). Anesthesia and transport of Brazilian flounder, Paralichthys orbignyanus, with essential oils of Aloysia gratissima and Ocimum gratissimum. Journal of the World Aquaculture Society, 43(1), 896-901.
Benovit, S. C., Silva, L. L., Salbego, J., Loro, V. L., Mallmann, C. A., Baldisserotto, B., Flores, E. M. M. & Heinzmann, B. M. (2015). Anesthetic activity and bioguided fractionation of the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. in silver catfish Rhamdia quelen. Anais da Academia Brasileira de Ciências, 87(3), 1675-1689. (Br).
Bulgarian Food Safety Agency (2011). Instruction for application of the requirements for transport of live fish. https://www.bfsa.bg/userfiles/files/ZHOJKF/Instruction_transport_fish_final(1).pdf
Can, E. & Sumer, E. (2019). Anesthetic and sedative efficacy of peppermint (Mentha piperita) and lavender (Lavandula angustifolia) essential oils in blue dolphin cichlid (Cyrtocara moorii). Turkish Journal of Veterinary and Animal Science, 43 (1), 334-341.
Clarke, S. (2008). Families of compounds that occur in essential oils. In: Essential Chemistry for Aromatherapy. Churchill Livingstone, London, UK, 41-77.
Diyaware, M., Suleiman, S., Akinwande, A. & Aliyu, M. (2017). Anesthetic effects of clove (Eugenia aromaticum) seed extract on Clarias gariepinus (Burchell, 1822) fingerlings under semi–arid conditions. Journal of Agricultural Sciences, 62(4), 411-421.
Dung, X. & Khien, V. (1993). Essential oils of wood, root, flower and fruit of camphor tree. Tap Chi Duoc Hoc, 8-10 (Chn).
Freitas Souza, C., Baldissera, M., Baldisserotto, B., Heinzmann, B., Martos-Sitcha, J. & Mancera, J. (2019). Essential oils as stress-reducing agents for fish aquaculture: a review. Frontiers in Physiology, 10, 785.
Frizzo, C., Santos, A., Paroul, N., Serafini, L., Dellacassa, E., Lorenzo, D. & Moyna, P. (2000). Essential oils of camphor tree (Cinnamomum camphora Nees & Eberm) cultivated in Southern Brazil. Brazilian Archives of Biology and Technology, 43(3), 211-219 (Br).
Garcia, O., Barcellos, G. & Baldisserotto, B. (2015). Net oin fluxes and ammonia excretion during transport of Rhamdia quelen juveniles.Ciencia Rural, 45(1), 1854-1858 (Br).
Guo, S., Geng, Z., Zhang, W., Liang, J., Wang, Ch., Deng, Z. & Du, S. (2016). The chemical composition of essential oils from Cinnamomum camphora and their insecticidal activity against the stored product pests. International Journal of Molecular Sciences, 17(11), 1836-1845.
Harmon, S. (2009). Methods for reducing stressors and maintaining water quality associated with live fish transport in tanks: a review of the basics. Reviews in Aquaculture, 1, 58-66.
Javahery, S., Nekoubin, H. & Moradlu, A. H. (2012). Effect of anaesthesia with clove oil in fish (review). Fish Physiology and Biochemistry, 38(6), 1545-1552.
Kamble, A., Saini, V. & Ojha, M. (2014). The efficacy of clove oil as anesthetic in common carp (Cyprinus carpio) and its potential metabolism reducing capacity. International Journal of Fauna and Biological Studies, 1(6), 01-06.
Khumpirapang, N., Pikulkaew, S., Anuchapreeda, S. & Okonogi, S. (2018). Anesthetic activity of plant essential oils on Cyprinus carpio (Koi carp). Drug Discoveries and Therapeutics, 12, 21-30.
Maricchiolo, G. & Genovese, L. (2011). Some contributions to knowledge of stress response in innovative species with particular focus on the use of the anaesthetics. The Open Marine Biology Journal, 5(1), 24-33.
Mazandarani, M. & Hoseini, S. (2017). Anesthesia of juvenile Persian sturgeon, Acipenser persicus; Borodin 1897, by peppermint, Mentha piperita, extract – Anesthetic efficacy, stress response and behavior. International Journal of Aquatic Biology, 5(6), 393-400.
Metin, S., Didinen, B., Kubilay, A., Pala, M. & Aker, I. (2015). Determination of anesthetic effects of some medicinal plants on rainbow trout (Oncorhynchus mykiss; Walbaum, 1792). Journal of Limnology and Freshwater Fisheries Research, 1(1), 37-42.
Mishra, A., Dwivedi, S., Kishore, N. & Dubey, N. (1991). Fungistatic properties of essential oil of Cinnamomum camphora. International Journal of Pharmacognosy, 29(4), 259-262.
Netto, J., Oliveira, R. & Copatti, C. (2017). Efficiency of essential oils of Ocimum basilicum and Cymbopogum flexuosus in the sedation and anaesthesia of Nile tilapia juveniles. Anais da Academia Brasileira de Ciências, 89(4), 2971-2974 (Br).
Öğretmen, F. & Gökçek, K. (2013). Comparative efficacy of three anesthetic agents on juvenile African catfish, Clarias gariepinus (Burchell, 1822). Turkish Journal of Fisheries and Aquatic Sciences, 13(1), 51-56 (Tr).
Oliveira, C., Lemos, S., Silva, A., Souza, S., Albinati, A., Lima, A. & Coppati, C. (2019). Use of eugenol for the anaesthesia and transportation of freshwater angelfish (Pterophyllum scalare). Aquaculture, 513(1), 1-6.
Ostrensky, A., Dal Pont, G., Geraldine, G., Castilho-Westphal, G. & Pedrazzani, A. (2016). Use of clove, mint and camphor essential oils on confinement of clown anemonefish Amphiprion ocellaris (Cuvier 1830): anesthetic effects and influence on water quality. Journal of Aquaculture Research and Development, 7(11), 457-465.
Pandey, K., Bora, R., Deka, C., Rastaji, C. & Banch, A. (1997). Composition of the essential oil of the bark of Cinnamomum camphora. Journal of Medicinal and Aromatic Plant Sciences, 19(1), 408-409.
Park, I., Lee, T. & Lim, S. (2018). Anesthetic efficacy and physiological responses of clove oil on juvenile and adult red spotted grouper, Epinephelus akarra. Fisheries and Aquatic Sciences, 21(25), 1-6.
Pedrazzani, A. & Neto, A. (2014). The anaesthetic effect of camphor (Cinnamomum camphora), clove (Syzygium aromaticum) and mint (Mentha arvensis) essential oils on clown anemonefish, Amphiprion ocellaris (Cuvier 1830). Aquaculture Research, 47(3), 769-776.
Pragadheesh, V., Saroj, A., Yadav, A., Chanotiya, S., Alam, M. & Samad, A. (2013). Chemical characterization and antifungal activity of Cinnamomum camphora essential oil. Industrial Crops and Products, 49(1), 628-633.
Rezende, F., Pascoa, L., Vianna, R. & Lanna, E. (2017). Sedation of Nile Tilapia with essential oils: tea tree, clove, eucalyptus, and mint oils. Revista Caatinga Mossoro, 30 (2), 479-486 (Br).
Roohi, Z. & Imanpoor, M. R. (2015). The efficacy of the oils of spearmint and methyl salicylate as new anesthetics and their effect on glucose levels in common carp (Cyprinus carpio L., 1758) juveniles. Aquaculture, 437, 327-332.
Sampio, D. & Freire, A. (2016). An overview of stress physiology of fish transport: changes in water quality as a function of transport duration. Fishand Fisheries, 17(1), 1055-1072.
Teixeira, R., Souza, C., Sena, C., Baldisserotto, B., Heinzmann, M. & Copatti, E. (2018). Essential oils of Aloysia triphylla is effective in Nile tilapia transport. Boletim do Instituto de Pesca, 44(1), 17-24 (Br).
Tondolo, J., Amaral, L., Simoes, L., Garlet, Q., Schindler, B., Oliveira, T., Silva, B., Gomes, L., Baldisserotto, B., Mallman, C. & Heinzmann, B. (2013). Anesthesia and transport of fat snook Centropomus parallelus with the essential oil of Nectandra megapotamica (Spreng.). Neotropical Ichthyology, 11(1), 667-674.
Velisek, J., Svobodova, Z., Piaсkova, V., Groch, L. & Nepejchalova, L. (2005). Effects of clove oil an aesthesia on common carp (Cyprinus carpio). Veterinary Medicine, 50(1), 269-275.
Wanyang, S., Wei, H. & Guangyu, W. (1989). Study on chemical constituents of the essential oil and classification of types from Cinnamomum camphora. Acta Botanica Sinica, 31(3), 209-214 (Chn).
Yeh, Y., Ya-Li, S., Shei, S., Cheng, S., Huang, S., Lin, J. & Liu, C. (2009). Evaluation of the antibacterial activity of leaf and twig extracts of strout camphor tree, Cinnamomum kanehirae, and the effects on immunity and disease resistance of white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology, 27(1), 26-32 (Chn).
Yildiz, M., Eroldogan, T., Engin, K., Gulc, A. & Baltaci, A. (2013). Effects of dietary cottonseed and/or canola oil inclusion on the growth performance, FA composition and organ histology of the juvenile rainbow trout, Oncorhynchus mykiss. Turkish Journal Fish Aquatic Science, 13(1), 453-464.
Yoshida, T., Maraki, S. & Komatsu, A. (1969). Minor constituents of Japanese ho-leaf oil. Agricultural and Biological Chemistry, 33(1), 343-352.
Yousefi, M., Hoseini, S., Vatnikov, Y., Nikishov A. & Kulikov, E. (2018). Thymol as a new anesthetic in common carp (Cyprinus carpio): Efficacy and physiological effects in comparison with eugenol. Aquaculture, 495(1), 376-383.
Zahl, I. H., Samuelsen, O. & Kiessling, A. (2012). Anaesthesia of farmed fish: implications for welfare. Fish Physiology and Biochemistry, 38(1), 201-218.
Zaikov, A., Hubenova, T. & Iliev, I. (2008). Induction and recovery from anesthesia in pike Esox lucius L. exposed to clove oil. Bulgarian Journal of Agricultural Science, 14(2), 165-171.
Zaikov, A., Iliev I. & Hubenova, T. (2009). The efficacy of clove oil as an anesthetic for channel catfish (Ictalurus punctatus Raf.). Conference proceedings IV International Conference “Fishery”, Belgrade, 365-369.
Zhao, D. H., Ke, C. L., Qi, L., Wang, X. F., Wang, Q. & Li, L. D. (2017). Elimination kinetics of eugenol in grass carp in a simulated transportation setting. BMC Veterinary Research, 13(1), 1-6.
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