Assessing polymorphism within common bean (Phaseolus vulgaris L.) mutant lines originated from variety Mastilen 11b using Inter Simple Sequence Repeats markers
Keywords:
Phaseolus vulgaris L., mutant lines, ISSR markersAbstract
The common bean (Phaseolus vulgaris L.) is an economically important crop on a global scale, including in Bulgaria as well. It is an excellent source of vegetable protein and other nutrients. One of the methods of modern plant breeding aimed at creating genetic diversity in beans is induced mutagenesis. Molecular techniques are a suitable tool for characterizing induced mutations. The objective of this work is to evaluate the genetic diversity of a collection of EMS-induced mutant bean lines and to identify specific profiles, using ISSR molecular markers. The initial variety “Mastilen 11b” and 15 mutant lines from the collection of the Maritsa Vegetable Crops Research Institute are analyzed with 14 ISSR reactions. A total of 187 fragments with lengths from 190 bp to 3100 bp are generated. Three of the reactions (ISSR 6, ISSR 8, ISSR 9) led to a generation of monomorphic profiles, and the remaining eleven reactions (ISSR 1, ISSR 2, ISSR 2T, ISSR 3, ISSR 4, ISSR 5, ISSR 7, ISSR 10, ISSR 12, UBC 807, UBC 809) amplified polymorphic profiles. The polymorphism ranged from 18.65 % to 87.50 % in the analyzed genotypes. The results showed that the applied ISSR markers are effective for assessing the existing genetic diversity of the studied bean mutant lines.
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