Efficiency of different doses of insectofungicidal biopreparation (Gaupsin) against phytophthora of tomato in Adjara, Georgia

Authors

  • Otar Shainidze Batumi State University “Shota Rustaveli”, Agroecology and Plant Protection Department, Faculty of Technology, Batumi, Georgia Author
  • Shota Lamparadze Batumi State University “Shota Rustaveli”, Department of Agrotechnology, Faculty of Technology, Batumi, Georgia Author
  • Nodar Beridze Batumi State University “Shota Rustaveli”, Department of Agrotechnology, Faculty of Technology, Batumi, Georgia Author
  • Guram Chkubadze Batumi State University “Shota Rustaveli”, Agroecology and Plant Protection Department, Faculty of Technology, Batumi, Georgia Author
  • Giorgi Macharadze Batumi State University “Shota Rustaveli”, Agroecology and Plant Protection Department, Faculty of Technology, Batumi, Georgia Author

Keywords:

gaupsin, phytophthora, sprays, tomato varieties, yield

Abstract

During 2019 and 2020 the field study was conducted at In Khelvachauri district (village Sameba) of Adjara, Georgia. Blight caused by Phytophthora infestans and Ph. parasitica is an important disease of Tomato (Lycopersicon esculentum Mill.). Study was aimed to determine the efficacies of different doses of biopreparation of Gaupsin against Phytophthora of tomato. Five tomato varieties (Jina, Florida 47, Shady-Lady, Adlia and Choportula) were sown in five replications with one standard check in tunnel. Gaupsin were applied after 7 days intervals. Disease data was recorded after ten days interval from flowering stage to onward. Average yield of each variety was calculated afters pickings.Studies show that over both years, all doses of gaupsin reduce disease severity compared to untreated plants. The highest reduction in the disease was achieved by applying gaupsin 12 mg/ml and 14 mg/ml of water at an interval of 7, 14, 21 and 28 days. Plants had increased leaf, shoot, fruit and root biomass. Among the five cultivars tested, Jima produces higher yields (6150 g) per plant, followed by Florida 47 (6000 g) and Shady Lady (5150 g) compared to Choportula (4300 g) and Adlia (3750 g). Overall results revealed that weekly sprays of gaupsin at 12 mg/ml of water were cost effective and eco-friendly for the management of Phytophthora blight of tomato. Based on a general analysis, it is very clearly shown that the investigation is an important step towards developing plant bioprotection strategies for antifungal activity against the important phytopathogen. That can be beneficial for farmers and researchers who involve in agriculture.

References

Alexandrov, V. (2011). Efficacy of Some Fungicides Against Late Blight of Tomato. Bulg. J. Agric. Sci., 17, 465-469. (Bg).

Aroma, K., Imanaka, H. & Konsaka, M. (1964). Pyrrolnitrin, a New Antibiotic Substance, Produced by Pseudomonas. Agricultural and Biological Chemistry, 28, 575–582.

Bahariev, D., Velev, B. & Harizanov, A. (1988). Plant protection of vegetables. Zemizdat, Sofia, 391 (Bg).

Balko, O. I., Kiprianova, E. A., Kovalenko, A. G., Shepelevych, V. V. & Avdeeva, L. V. (2010). Antiphytoviral activity of gaupsin biopreparat. Microbiol. and Biotechnology, 2, 51–57.

Bernd, H. & Rehm, A. Pseudomonas (2008). Model Organism, Pathogen, Cell Factory. Wiley-Blackwell, 395.

Carlier, E., Rovera, M., Rossi Jaume, A. & Rosas, S. B. (2008). Improvement of growth, under field conditions, of wheat inoculated with Pseudomonas chlororaphis subsp. aurantiaca SR1. World Journal of Microbiology and Biotechnology, 24, 2653-2658.

Chincholkar, S. & Tomashow, L. (2013). Microbial Phenazines Biosynthesis, Agriculture and Health. Springer, Berlin, Heidelberg, 248.

Duffy, B. K. & Défago, G. (1999). Environmental Factors Modulating Antibiotic and Siderophore Biosynthesis by Pseudomonas fluorescens Biocontrol Strains. Applied and Environmental Microbiology, 65, 2429-2438.

Gabrilovich, I. M. (1989). On the inhibitory effect of Bacillus subtilis and B. Brevis on putrefactive microorganisms during storage of vegetables and fruits. In: Abstracts of the conference “Microorganisms-stimulants and inhibitors of growth of plants and animals.” Tashkent, 8.

Gondal, A. S., Ijaz, M., Riaz, K. & Khan, A. R. (2012). Effect of Different Doses of Fungicide (Mancozeb) against Alternaria Leaf Blight of Tomato in Tunnel. Journal of Plant Pathology & Microbiology, 3, :3.

Goral, V. M., Lappa, N. V., Goral, S. V., Garagulia, A. D., Kiprianova, E. A., Omelianets, T. & Smirnov, V. V. (1999). The insect – fungicidal preparation, gaupsin, isolated from Pseudomonas aureofaciens strains. Prikl Biokhim Mikrobiol, 35(5), 596-8.

Horneburg, B. & Becker, H. C. (2011). Selection for Phytophthora field resistance in the F2 generation of organic outdoor tomatoes. Euphytica, 180, 357 – 367.

Kiprianova, E. A., Klochko, V. V., Zelena, L. B., Churkina, L. N. & Avdeeva, L. V. (2011). Pseudomonas batumici sp. nov., the antibiotic-producing bacteria isolated from soil of the Caucasus Black Sea coast. Mikrobiology, 73 (5), 3-8 (Ru).

Kiprianova, E. A. & Safronova, A. O. (2017). Antimicrobial, entomopathogenic and antiviral activity of gaupsin biopreparation created on the basis of Pseudomonas chlororaphis strains Prosyanov. Biotechnologia Acta, 10, 7-16 (Ru).

Kuznetsov, A. E. & Gradova, N. B. (2006.). Scientific basis of environmental biotechnology. (Textbook), Moscow, Mir, 504 (Ru).

Mandryk, M. N., Kolomiets, E. I. & Dey, E. S. (2007). Characterization of antimicrobial compounds produced by Pseudomonas aurantiaca S-1. Polish Journal of Microbiology, 56, 245-250.

Mateeva, A., Braikova, B. & Avdjiiski, G. (1985). Diseases and pest on potato. Zemizdat, Sofia, 101 (Bg).

Mercado-Blanco, J. & Bakker, P. (2007). Interactions between plants and beneficial Pseudomonas spp.: exploiting bacterial traits for crop protection. Antonie van Leeuwenhoek, 92 (4), 367–89.

Mehnaz, S. (2013). Secondary metabolites of Pseudomonas aurantiaca and their role in plant growth promotion. In: Plant Microbe Symbiosis: Fundamentals and Advances. Berlin-Heidelberg. Germany, Springer, 373-393.

Mikeladze, Z., Kutaladze, N. & Lominadze, Sh. (2021). Influence of the biopreparation “Gaupsin” on storability of mandarin fruits. International Journal of Scientific Research and Management (IJSR), 09, 304-312

Nagornaya, L. V. (2015). Main diseases of apricot and biological control of their distribution in conditions of the southern steppe of Ukraine. Journal “Scientific works of the North Caucasian Zonal Scientific Research Institute of Horticulture and Viticulture”, 8, 183-188 (Ru).

Nakov, B., Nakova, M., Angelova, R. & Andreev, R. (2007). Forecasted and signalization of diseases and pest on crop plants. Publisher IMN-Plovdiv, 434 (Bg).

Olaniyi, J. O., Akanbi, W. B., Adejumo, T. A. & Akande, O. G. (2010). Growth, fruit yield and nutritional quality of tomato varieties. African Journal of Food Science, 4, 398-402.

Rosas, S. B., Altamirano, F., Schroder, E. & Correa, N. (2001). In vitro biocontrol activity of Pseudomonas aurantiaca. Phyton-International Journal of Experimental Botany, 67, 203- 209

Rosas, S., Rovera, M., Andrés, J. A., Pastor, N. A., Guiñazú, L. B. & Carlier, E. (2005). Characterization of Pseudomonas aurantiaca as biocontrol and PGPR agent. In: Endophytic properties. Proceeding prospects and applications for plant associated microbes, 1st International conference on plant– microbe interactions: endophytes and biocontrol agents. Lapland, Finland: Sorvari, 91-99.

Rosas, S. B., Andrés, J. A., Rovera, M. & Correa, N. S. (2006). Phosphate solubilizing Pseudomonas putida can influence the rhizobia–legume symbiosis. Soil Biology & Biochemistry, 38, 3502-3505.

Rosas, S. B., Avanzini, G., Carlier, E., Pasluosta, C., Pastor, N. & Rovera, M. (2009). Root colonization and growth promotion of wheat and maize by Pseudomonas aurantiaca SR1. Soil Biology and Biochemistry, 41, 1802–1806.

Rosas, S. B., Carlier, J.A., Andrés, J., Bergesse, L.B., Guiñazú, V., Vogt, V., Bergesse, J. & Rovera, M. (2012). Efficacy of Pseudomonas chlororaphis subsp. aurantiaca SR1 for improving productivity of several crops. Crop Production Technologies, 178-270.

Rovera, M., Andres, J., Carlier, E., Pasluosta, C., Avanzini, G. & Rosas, S. (2008). Pseudomonas aurantiaca: plant growth promoting traits, secondary metabolites and inoculation response. Plant–bacteria interactions. In: Strategies and Techniques to Promote Plant Growth, Germany: Wiley– VCH; 155-164.

Rovera, M., Pastor, N., Niederhauser, M. & Rosas, S. B. (2014). Evaluation of Pseudomonas chlororaphis subsp. aurantiaca SR1 for growth promotion of soybean and for control of Macrophomina phaseolina. Biocontrol Science and Technology, 24 (9), 1012- 1025.

Shainidze, O. T. (1999). Mycobiota of Adjara. Batumi, Georgia, 355 (GE).

Shainidze, O. T. ( 2014). The results of phytopathological research in Adjara. Tbilisi, Georgia, 304 (GE).

Shainidze, O. T. & Ghoghoberidze, S. (2015). Dominant pathogenic of Lycopersicum esculentum Mill. International Journal of Agricultural Science Research, 4(5), 092-097.

Shahid, I., R.ivan, M., Baig, D. N., Saleem, R. S., Malik, K. A., & Mehnaz, S. (2017). Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass. J. Microbiol Biotechnol., 27(3), 480– 491.

Shanahan, P., Sullivan, D. J. O., Simpson, P., Glennon, J. D. & Gara F. O. (1992). Isolation of 2,4-diacetylphloroglucinol from a fluorescents Pseudomonas and investigation of physiological parameters influencing its production. Applied and Environmental Microbiology, 58, 353-358.

Sgherri, C., Kadlecova, Z., Pardossi, A., Navari-Izzo, F. & Izzo, R. (2008). Irrigation with diluted seawater improves the nutritional value of cherry tomatoes. Journal of Agricultural and Food Chemistry, 56, 3391-3397.

Steel, R. G. D., Torrie, J. H. & Dicky, D. A. (1997). Principles and Procedures of Statistics, A Biometrical Approach. 3rd Edition, McGraw Hill, Inc. Book Co., New York, 352-358.

Tombolini, R., Gaag, D. J., Gerhardson, B. & Jansson, J. K. (1999). Colonization Pattern of the Biocontrol Strain Pseudomonas chlororaphis MA 342 on Barley Seeds Visualized by

Using Green Fluorescent Protein. Applied and Environmental Microbiology, 65 (8), 3674–3680.

Weller, D. M. (2007). Pseudomonas Biocontrol Agents of Soilborn Pathogens: Looking Back over 30 years. Phytopathology, 97(2), 250-6.

Wheeler, B. J. (1969). An Introduction to Plant Diseases. John Wiley and Sons, Ltd., 374.

Downloads

Published

24.06.2022

How to Cite

Shainidze, O., Lamparadze, S., Beridze, N., Chkubadze, G., & Macharadze, G. (2022). Efficiency of different doses of insectofungicidal biopreparation (Gaupsin) against phytophthora of tomato in Adjara, Georgia. Bulgarian Journal of Agricultural Science, 28(3), 437-442. https://agriacad.eu/ojs/index.php/bjas/article/view/1590