Genetic polymorphism of the melatonin receptor (MTNR1A) gene in populations of fine-fleece sheep breeds in Bulgaria
Keywords:
sheep, reproduction, PCR, RFLP, MTNR1A gene, polymorphismAbstract
In the present study was investigated the single nucleotide polymorphism in exon II of the melatonin receptor (MTNR1A) gene in a total of 90 individuals representing three fine fleece sheep breeds:
Caucasian Merino, Ascanian Merino and Karnobat Merino. By means of PCR-RFLP analysis two allele variants of MTNR1A gene (C and T) and three genotypes (CC, CT and TT) have been identified.
The obtained genotype frequencies of alleles C and T in the investigated sheep breeds were: 0.60 and
0.40 in Caucasian Merino sheep, 0.47 and 0.53 in Askanian Merino sheep, 0.60 and 0.40 in Karnobat
Merino sheep, respectively. The genetic diversity at MTNR1A gene was with the highest value in the
population of Ascanian Merino (Ho
= 0.533 и He = 0.498). The lowest coefficient of inbreeding (Fis
= -0.070) was also observed in this group and it was in correspondence with HWE equilibrium (p =
0.4). The frequency of mutant allele T was higher (0.53) than the wild allele C (0.47). In Caucasian and
Karnobat Merino breeds the allele frequencies were the same – 0.60 and 0.40 for allele C and allele
T, respectively. Despite the same allelic frequencies, the two breeds had different distributions in the
genotype frequencies, with the most significant difference in the heterozygous genotype CT – 0.40
for Caucasian Merino and 0.27 for Karnobat Merino. The population of Karnobat Merino sheep breed
was not consistent with HWE with p = 0.02. The results in this study showed polymorphism in exon II
of the ovine MTNR1A gene in all tested breeds. Due to that fact, it could be concluded that this gene
is suitable for genetic marker.
References
Fathy, H. A., Gouda, E. M., Gafer, J. A., Galal, M. K., & Nowier, A. M. (2018). Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds. Archives Animal Breeding, 61(4), 505-516.
Messer, L. A., Wang, L., Tuggle, C. K., Yerle, M., Chardon, P., Pomp, D., ... & Rothschild, M. F. (1997). Mapping of the melatonin receptor la (MTNR1A) gene in pigs, sheep, and cattle. Mammalian genome, 8(5), 368-370.
Saxena, V. K., Jha, B. K., Meena, A. S., Narula, H. K., Kumar, D., & Naqvi, S. M. K. (2015). Melatonin receptor 1A (MTNR1A) gene sequence characterization and SNP identification in tropical sheep breeds of India. Receptors & Clinical Investigation, 2. 1–5.
Ahmed, A. M. (2008). Biological evaluation of Barki sheep under two different breeding seasons. Egyptian Journal of Animal Production, 45(1), 15-24.
Giantsis, I. A., Laliotis, G. P., Stoupa, O., & Avdi, M. (2016). Polymorphism of the melatonin receptor 1A (MNTR1A) gene and association with seasonality of reproductive activity in a local Greek sheep breed. Journal of Biological Research-Thessaloniki, 23(1), 1-4.
Držaić, V., Ramljak, J., Kasap, A., Širić, I., Antunović, Z., & Mioč, B. (2020). MTNR1A gene polymorphisms in Istrian sheep population. 55. hrvatski i 15. međunarodni simpozij agronoma, 2020 godine, Vodice, Hrvatska, 16.-21. veljače 2020. Zbornik radova, 424-428.
Gencheva, D. (2019). Genetic polymorphism of melatonin receptor (MTNR1A) gene in populations of local sheep breeds. Zhivotnovadni Nauki, 56(1), 21-32 (Bg).
Cosso, G., Nehme, M., Luridiana, S., Pulinas, L., Curone, G., Hosri, C., ... & Mura, M. C. (2021). Detection of Polymorphisms in the MTNR1A Gene and Their Association with Reproductive Performance in Awassi Ewes. Animals, 11(2), 583-590.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2024 Bulgarian Journal of Animal Husbandry
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
