PROPORTION OF THE SHORT, MEDIUM AND LONG - FATTY ACIDS IN THE RUMEN AND DUODENAL CONTENT OF YEARLING RAMS

Abstract

The purpose of the study is to determine the effect of the multienzymatic preparations Hostazym C 100 and Hostazym X 100 on the proportion of fatty acids with the length of hydrocarbon chain from C4 to C18 in rumen fluid and chyme from cranial part of duodenum in yearling rams, fed with rations including different protein and lipid level. It has been conducted an experiment with nine male fistulated yearling rams, Blackhead Pleven bread, with average live weigh of 45.200 kg at the beginning of the trial. The animals are raised three into groups and housed in individual stalls. The study was organized into three periods - control and two experimental. During the control period, the first group yearling rams was fed ration consist of 1kg of barley and 1 kg of hay meadows, the second group - of 0.800 kg of barley, 0.200 kg of sunflower meal and 1 kg of hay meadow and the third group - with a diet consisting 0.800kg of barley, 0.200 kg of sunflower expeller and 1 kg of meadow hay. During the first trial period to the rations of the three groups of animals was added enzyme preparation Hostazym C 100 at a dose 1g/kg concentrated feed, mixed with concentrate 10 days before feeding. In the second trial period to the concentrated feed in the three groups tested was added enzyme preparation Hostazym X 100, at the same dose and route of administration. It has been found, that Hostazym C 100 and Hostazym X 100 decreased the amount unsaturated fatty acids in rumen content of yearling rams, fed with rations consist 2.52% lipids (barley, sunfl. ecspeller and meadow hay) (Р< 0.05 - 0.001). In the thee exanimated rations Hostazym X 100 increased the percentage of the saturated fatty acids in the rumen fluid of the yearling rams before feeding (Р<0.05-0.001). The enzyme preparation caused the same effect two and a half hours after feeding in ration I (barley and meadow hay) and in ration III (barley, sunfl. expeller and meadow hay) (Р<0.05-0.001). The researched enzymatic preparations increased relative amount of the unsaturated fatty acids, decreased the quantity of the saturated fatty acids before feeding and caused increasing of the total percentage of the fatty acids with odd number of carbon atoms and with branched hydrocarbon chain 2.5 h after feeding in the duodenal content of the yearling rams, fed with the equal proportions of concentrate and roughage, regardless of protein and lipid levels (from unreliable to P<0.001).