RESEARCH ON LDH-X AND SPERM MOTILITY IN OVIS ARIES SPECIES
Abstract
Current study established relationship between LDH-X activity and motility in ram spermatozoa. In the experiment were used 12 ejaculates from 6 clinically healthy rams, equilibrated on age, feeding and farming. Sperm parameters was determined on Sperm Class Analyzer (Microptic Systems). The biochemical activity and electrophoretical profile of LDH-X were investigated in water and Triton X-100 soluble extract from ram spermatozoa. The obtained results shown, that ejaculates with higher percent motile spermatozoa had higher activity of LDH-X.
References
Захариев, З., 1986. Проучвания върху биологичната пълноценност на семенна течност при видовете Bos taurus and Ovis aries, Дисертация ИБИР, София, 127-148.
Йонков, Й., 1986. Цитохимично проучване на НАДН-ТР и лактатдехидрогеназа в сперматозоиди на човек. Автореферат на дисертация, Медицински факултет, Варна, 14-23
Йонков, Й., М. Събев, П. Цветкова, 1999. Хистохимично изследване на лактатдехидрогеназата в нормарни следпубертетни тестиси на човек. ІІ Национален конгрес
Цветкова, П., 2000. Постпаротиден орхид и инфертилитет. НФ Андрология. С, 65.
Цветкова, П., Д. Цветков, М. Събев, 1999. Сперматологични и ензимоцитохимични маркери на хроничен неспецифичен простатит и инфертилитет. Урология, 4,122-126.
Allison, A. and E. Hartree, 1996. Lysosomol nature of the acrosomes of ram spermatozoa. Biochem. J. III, 35-36.
Apella, D. and C. Markert, 1961. Biochem. Biophis. Res. Communs., 6, 171.
Blanco, A. and Zinkham W. H., 1963. Lactatedehydrogenase in human testis. Science,153, 139-601
Brancaccio, F., M. Limongelli and N. Maffulli, 2006. Monitoring of serum enzymes in sport British Journal of Sports Medicine; 40:96-97;
Brooks ,G. A., 2001. Lactate shuttles in Nature Biochem. Soc. Trans. 30, (258–264)
Burgos, C., N. Gerez de Burgos, C. Coronel and A. Blanco, 1979. Substrate specificity of lactate dehydrogenase isoenzyme C, from human spermatozoa and a possible selective assay. J Repard Fertil. 55, 101-108.)
Duan, C., Goldberg E., 2003. Inhibition of lactate dehydrogenase C4 (LDH-C4) blocks capacitation of mouse sperm in vitro.Cytogenet Genome Res, 103:352-359.
Gavella, M. and Cvitkovic, P.,1985. Semen LDH-X deficiency and male infertility. Arch. of Andrology
Goldberg E., 1974. The seminal fluid izoenzyme LDH-C in infertile men. Biology of Reproduction Vol 18, 120-121
Mann, T., 1964. The biochemistry of semen and of the male reproductive track, Neuthuen, London, 187.
O’Flaherty, C. M., N. B. Beoriequi and M. T. Beconi, 2002. Lactate dehydrogenase –C4 is involved in heparinapd NADH – dependent bovine sperm capacitation (Abstract), Andrologia, , 34, 91.
Tosic, Y. and Walton, A.,1946. Formation of hydrogen peroxide by spermatozoa and its inhibitory effect on respiration. Nature, 158, 485.
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