STRESS SYNDROME (PSS) AND PHYSICALCHEMICAL COMPOSITION OF M. LONGISSIMUS DORSI IN PIGS OF EAST BALKAN BREED
Keywords:
East Balkan breed pigs, ryanodine receptor gene, stress syndromeAbstract
The Malignant hyperthermia in pigs is due to a mutation in the locus of the ryanodine receptor
gene (RYR1) or Porcine stress syndrome (PSS) and the presence of pale, soft and exudative (PSE) meat. The aim of this study is to make genotyping for ryanodine receptor gene and physicalchemical
analysis of m. Longissimus dorsi in pigs from East Balkan breed. Genotyping by searches locus was
performed by polymerase chain reaction method (PCR method) and the analysis of the polymorphism of the restriction fragment lengths (RFLP). DNA is isolated from plucked hair of 32 animals from two herds bred in Burgas and Shumen region. Analyses of genotyping at RYR1 locus from pigs of East Balkan breed showed only homozygous for the dominant allele individuals (NN). The mutation of RYR1 gene (recessive allele n) has not been observed and the tested animals are negative for porcine stress syndrome. Established phenotype of pale, soft and exudative meat in m. Longissimus dorsi at East Balkan breed pigs after induced stress that is by non-genetic origin.
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