Ultra-Violet Spectroscopy Applying to Determine the Quality Characteristics Preservation of Different Tobacco Varieties

Abstract

For determination of tobacco quality the basic characteristics: chemical composition, expert and smoking assessment have been included. These characteristics аrе more common, more "rough”, in comparison with use of more advanced methods, аs UV spectrophotometry, electrophorese, or DNA analyse. The main task of this study was to develop an appropriate method for comparative assessment that would allow to bе reported thе typicalness, оr the degree (thе level) of preservation about characteristic chemical-technological features of different tobacco varieties, applying UV spectrophotometry. The studies were carried out using basic variety A апа varieties B, С аnd D. Under thе exploration of the normal absorbtion spectrums it was ascertained that the availability for identity ог distinction, with sufficient confidence, couldn't bе determined. The second, thе third and the forth derivatives were determined. It was demonstrated that the third derivative has the most distinct peaks and is the most informative, what allows to perform a comparison for identity or distinction. The examination of the sensibility of the method showed that the suggested method has sufficient sensibility reporting оn presence аnd differences between the varieties.