A Study for the Influence of Zeolite, Modified with Cu, Zn and Mn, on the Biotransformation of Lignite
Keywords:
lignite, humic substances, Trichoderma harzianum, hypocotyls testAbstract
Abiotransformation оf humic substances from coal was carried out during а solid-state fermentation of lignite from Stanyantsi mine, Bulgaria, inoculated with Trichoderma harzianum, in the presence of natural clinoptilolite, saturated with increasing doses of Cu, Zn and Mn.
The level of extractable humic substances reaches that typical for leonardite (80% of the total organic C). The content of fulvic acids in the hydrolysate is increased up to 4 times compared to the raw material. Cu ions, which are toxic to Trichoderma sp. at 50 ррm, can be introduced into the substrate for fermentation in а tenfold greater amount, if used together with the zeolites. The presence of Zn and Мn ions during the process of depolymerization supports lignite biotransformation. This results in obtaining products with a high physiological activity, affecting plant phytochrome system.
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