Effect of Humic Substances on the Structural Stability of Proteins and Resistance of Erythrocytes Against Autohemolysis
Keywords:
humic substances, autohemolytic resistance, protein stabilityAbstract
Peat and peat extracts are extensively studied for their therapeutic effects. Water solution of peat (WTE) and four peat ingredients, humic acid (HA), fulvic acid (FA), uvic acid (UA), and a fraction (NaP) obtained through extraction with Na phosphate, were used in this study. Their effects on the autohemolysis of human erythrocytes and on the structural stability of fibrinogen, represented by its temperature, td аnd enthalpy, ΔH, of denaturation, determined through UV-spectroscopy were compared. All peat ingredients reduced ΔH, the reductions ranked their ability to bind the protein in the order: FA, WTE > UA >> HA >NaP. FA and HA accelerated autohemolysis while UA, NaP and WTE expressed beneficial impact on the erythrocyte membrane inhibiting autohemolysis.
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