Response of Alfalfa (Medicago sativa L.) to microbial inoculations with Rhizobium meliloti, Pseudomonas fluorescens and Glomus intraradices
Keywords:
alfalfa, symbiotic N-fixing bacteria, AM (Arbuscular mycorrhizal) fungus, phosphate solubilizing bacteria, yield, nitrogen (N) and phosphorus (P) uptakeAbstract
The paper is aimed at establishing the effect of inoculation of alfalfa (Medicago sativa, L.) with N-fixing bacteria Rhizobium meliloti, mycorrhizal fungus Glomus intraradices and phosphate solubilizing bacterium Ps. fluorescens on the yield and N and P content of aboveground biomass. The plants were grown on Chromic Luvisol in a two-year field experiment under irrigation regime. Two cuts were harvested in the first year and four - in the second year. Positive effect of single and double microbial inoculations with Rh. meliloti, Ps. fluorescens and Gl. intraradices was established in both years. The largest increase in plant biomass yield and N uptake by the shoots was obtained with the single inoculation with Rh. meliloti 225 and mixed inoculation with Rh. meliloti 116 + Ps. fluorescens. The increases of alfalfa yield for these treatments were 29 and 34% for the first year and 32 and 27% for the second year respectively. The P uptake of aboveground biomass was the highest in the treatments with mixed inoculations of Rh. meliloti 116 with Ps. fluorescens or Gl. intraradices. The results obtained demonstrate the positive effect of microbial inoculations on the yield and nutrient uptake of alfalfa and could be of practical benefit for sustainable agricultural practices.
References
Adesemoye, A. O., & Kloepper, J. W. (2009). Plant–microbes interactions in enhanced fertilizer-use efficiency. Applied microbiology and biotechnology, 85(1), 1-12.
Bremner, J., & Mulvaney, M. (1982). Nitrogen-total. In: Methods of Soil Analysis, Part 2, Madison, Misconsin, USA, 595-622.
Djonova, E., & Petkova, G. (2014). Effect of Inoculation with AM-fungi and N-fixing Microorganisms on Plant Biomass of Forage Grasses. Journal of Balkan Ecology, 17(3), 261-269.
Guiñazú, L. B., Andrés, J. A., Del Papa, M. F., Pistorio, M., & Rosas, S. B. (2010). Response of alfalfa (Medicago sativa L.) to single and mixed inoculation with phosphate-solubilizing bacteria and Sinorhizobium meliloti. Biology and fertility of soils, 46(2), 185-190.
Hungria M., Nogueira, M., & Araujo, R. (2013). Co-inoculation of Soybeans and Common Beans with Rhizobium and Azospirillum: Strategies to Improve Sustainability. Biology and Fertility of Soils, 49, 491-501.
IUSS Working Group WRB. (2006). World Reference Base for Soil Resources. 2nd edition World Soil Resources Reports 103. FAO, Rome, p. 128.
Jafari, M., Yari, M., Ghabooli, M., Sepehri, M., Ghasemi, E., & Jonker, A. (2018). Inoculation and Co-inoculation of Alfalfa Seedlings with Root Growth promoting Microorganims (Piriformospora indica, Glomus intraradices and Sinorhizobium melliloti) Affect Molecular Structure, Nutrient Profiles and Availability of Hay for Ruminants. Animal Nutrition, 4(1), 90-99.
Kirilov, A. (2016). Role of Leguminous Fodder Crops for Sustainable Agriculture. Journal of Mountain Agriculture on the Balkans, 19(2), 46-84.
Kravchenko, I., Kizilova, A., Titova, L., & Iutinskaya, G. (2013). Effect of Microbial Fertilizers on Rhizospheric Bacterial Diversity and Yield Response of Soybean Plants. Agricultural Science Developments, 2(12), 120-125.
Mincheva, M., & Brashnarova, A. (1975). Certain Ways of Mineralization of Plant Material by Routine Analysis to Determine P, K, Ca, Mg, Na, Zn, Mn, Cu and Fe by the Methods of Contemporary Spectrophotometry. Soil Science and Agrochemistry, 10, 114-123.
Petkova, G., Nedyalkova, K., Perfanova, J., & Eftimova, D. (2018). Effect of microbial inoculants on plant biomass of lucerne. Journal of Balkan Ecology, 21(4), 370-377.
Plazinski, J., & Rolfe, B. (1985). Influence of Azospirillum Strains on the Nodulation of Clovers by Rhizobium Strains. Applied and Environmental Microbiology, 49, 984-989.
Singh, J. S., Pandey, V. C., & Singh, D. P. (2011). Efficient soil microorganisms: a new dimension for sustainable agriculture and environmental development. Agriculture, ecosystems & environment, 140(3-4), 339-353.
Stajkovic, O., Delic, D., Josic, D., Kuzmanovic, D., Rasulic, N., & Knezevic-Vukcevic, J. (2011). Improvement of common bean growth by co-inoculation with Rhizobium and plant growth-promoting bacteria. Romanian Biotechnological Letters, 16(1), 5919-5926.
Stajković-Srbinović, Оlivеra., Delić, Dušica., Nerandžić, Boris., Andjelović, Srdjan., Sikirić, Biljana., & Kuzmanović Dj, R. N. (2017). Alfalfa yield and nutrient uptake as influenced by co-inoculation with rhizobium and rhizobacteria. Rom Biotech Lett, 22(4), 12834-41.
Stancheva, I., M. Geneva, E. Djonova, N. Kaloyanova, M. Sishanova, M. Boychinova, G. Georgiev. (2008). Response of Alfalfa (Medicago sativa, L.) Growth at Low Accessible Phosphorus Source to the Dual Inoculation with Mycorrhizal Fungi and Nitrogen Fixing Bacteria. General and Applied Plant Physiology, 34(3-4), 319-326.
Subba Rao, N. S. (1993). Biofertilizers in Agriculture and Forestry, Oxford and IBH Co, New Delhi, p. 208.
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