The impact of genotype, environment, and genotype × environment interaction on wheat grain yield and quality
Keywords:
wheat; genotype x environment; grain yield; grain quality; correlationsAbstract
Objects and purpose: In a multifactor field experiment, common winter wheat cultivars developed in Bulgaria were studied. The main factors site (location), season (year) and variety (genotype), the combination of which determines the magnitude and change of the studied characters, have been studied in detail. The aim is to establish and analyze all aspects of the impact of these factors separately, as well as their interaction on yield and several parameters of grain quality. The working hypothesis of the purpose is related to establishing whether there is a sufficiently different magnitude and direction of "genotype x environment" interaction (GE), which can become a prerequisite for a correct assessment of the performance and stability of each variety of the group.
Methods and approaches: Wheat varieties were studied during the period (2017-2019) in four locations of the country: Dobrich, Plovdiv, Trastenik, (Russe) and V. Tarnovo. Five grain characteristics were analyzed - Grain Yield (GY), Test Weight of grain (TW), Wet Gluten content of grain (WGC), Grain Protein Content (PC), and Grain Gluten Index (GI). In the statistical analyses, the parameters indicating the quality were taken as quantitative traits. All possible aspects of the „genotype x environment” interaction were investigated using several specialized statistical programs. The changes in the interrelationships between the traits in dynamically changing environmental conditions (location and year) were determined.
Key results: Genotype-environment (GE) interactions were found for all traits studied. Traits are differentially influenced by these factors, with GI being the most genetically dependent and GY the least. The full GGE interaction accounts for complex directional and magnitude variation of traits by location and variety. The interactions between the factors show a linear (PC1) and non-linear nature (PC2 - PC4), which is the reason for the observed changes in the mean values of each characteristic being statistically reliable in some of the tested varieties. The conditions in the locations differ significantly, which creates prerequisites for the maximum possible expression of GGE in each of the trait, without exception. They cause the established changes in the correlations between the yield and the examined parameters of grain quality, according to the test location.
Conclusions: The influence of the "environment" and "genotype" factors for each trait is different as a share of their total variation. The "genotype x environment" interaction is an essential factor that affects all the traits studied. Its full expression designated as GGE is different depending on the genetics of the trait (G) and its tendency to substantial change (GE) in the specific conditions of the locations. Fluctuations in the mean values of the studied varieties, relative to the environmental conditions, allow their correct comparison according to each of the traits.
References
Alava, J., Millar, S., & Salmon, S. (2001). The determination of wheat breadmaking performance and bread dough mixing time by nir spectroscopy for high speed mixers. Journal of Cereal Science, 33(1), 71–81. https://doi.org/10.1006/jcrs.2000.0341
Alemu, G., & Gerenfes, D. (2021). Effect of genotype by environment interactions on quality traits of bread wheat in ethiopia. Asian Journal of Plant Science and Research, 11(1), 1-9.
Bhatta, M., Regassa, T., Rose, D. J., Stephen Baenziger, P., Eskridge, K. M., Santra, D. K., & Poudel, R. (2018). Genotype, environment, seeding rate, and top-dressed nitrogen effects on end-use quality of modern Nebraska winter wheat. Journal of the Science of Food and Agriculture, 98(7), 2830. https://doi.org/10.1002/jsfa.8916
Bonfil, D., & Posner, E. (2012). Can bread wheat quality be determined by gluten index? Journal of Cereal Science, 56(2), 115–118. https://doi.org/10.1016/j.jcs.2012.07.003
Clarke, F. R., Clarke, J. M., Ames, N. A., Knox, R. E., & Ross, R. J. (2010). Gluten index compared with SDS-sedimentation volume for early generation selection for gluten strength in durum wheat. Canadian Journal of Plant Science, 90(1), 1–11. https://doi.org/10.4141/cjps09035
Dencic, S., Kobiljski, B., Mladenov, N., Hristov, N., & Pavlovic, M. (2007). Long-term breeding for bread making quality in wheat. In: Wheat production in stressed environments, Springer, pp. 495-501.
Dencic, S., Mladenov, N., & Kobiljski, B. (2011). Effects of genotype and environment on breadmaking quality in wheat. International Journal of Plant Production, 5(1), 71–81. https://doi.org/10.22069/ijpp.2012.721
Desheva, G., & Deshev, M. (2021). Evaluation of the stability and adaptability of yield in varieties and breeding lines of common winter wheat. Rastenievadni nauki, 58(1), 3-13 (Bg). https://cropscience-bg.org/page/en/details.php?article_id=903
Desheva, G., (2016). Effects of genotype, environment and their interaction on quality characteristics of winter bread wheat. Journal of Basic Applied Research, 2(3), 363-372.
Dimitrov, E., Uhr, Z. & Chipilski, R. (2022). Study of yield and stability by common winter wheat varieties by changing climatic conditions in Sadovo region. Bulgarian Journal of Agricultural Science, 28 (2), 271–278.
Eichi, V. R., Okamoto, M., Garnett, T., Eckermann, P., Darrier, B., Riboni, M., & Langridge, P. (2020). Strengths and weaknesses of national variety trial data for multi-environment analysis: a case study on grain yield and protein content. Agronomy, 10(5), 753. https://doi.org/10.3390/agronomy10050753
Gauch, H. G. (1988). Model selection and validation for yield trials with interaction. Biometrics, 44(3), 705. https://doi.org/10.2307/2531585
Gollob, H. F. (1968). A statistical model which combines features of factor analytic and analysis of variance techniques. Psychometrika 33, 73–115 https://doi.org/10.1007/BF02289676
Gómez-Becerra, H. F., Abugalieva, A., Morgounov, A., Abdullayev, K., Bekenova, L., Yessimbekova, M., & Cakmak, I. (2010). Phenotypic correlations, G× E interactions and broad sense heritability analysis of grain and flour quality characteristics in high latitude spring bread wheats from Kazakhstan and Siberia. Euphytica, 171(1), 23-38. https://doi.org/110.1007/s10681-009-9984-6
Gubatov, T., Yanchev, I., & Tsenov, N. (2016). Effect of the environments on the productivity-related characters in common winter wheat. Bulgarian Journal of Agricultural Science, 22(6), 927-935. https://journal.agrojournal.org/page/en/details.php?article_id=278
Guzmán, C., Autrique, J. E., Mondal, S., Singh, R. P., Govindan, V., Morales-Dorantes, A., Posadas-Romano, G., Crossa, J., Ammar, K., & Peña, R. J. (2016). Response to drought and heat stress on wheat quality, with special emphasis on bread-making quality, in durum wheat. Field Crops Research, 186, 157–165. https://doi.org/10.1016/j.fcr.2015.12.002
Herrera, J. M., Häner, L., Mascher, F., Hiltbrunner, J., Fossati, D., Brabant, C., Charles, R., and Pellet, D. (2020). Lessons from 20 years of studies of wheat genotypes in multiple environments and under contrasting production systems, Frontiers in Plant Science, 10. https://doi.org/10.3389/fpls.2019.01745
Horvat, D. Šimi´c, G., Dvojkovic, K., Ivic., M., Plavšin, I. & Novoselovic, D. (2021). Gluten protein compositional changes in response to nitrogen application rate. Agronomy, 11, 325. https://doi.org/10.3390/agronomy11020325
Jernigan, K.L., Godoy, J. V., Huang, M. Z., Y, Morris, C. F., Garland-Campbell, K. A., Zhang, Z., & Carterр A. H. (2018). Genetic dissection of end-use quality traits in adapted soft white winter wheat, Frontiers in Plant Science, 9, 271. https://doi.org/10.3389/fpls.2018.00271
Johansson, E., Branlard, G., Cuniberti, M., Flagella Z., Hüsken A., Nurit, E., Peña, R. J., Sissons, M. & Vazquez, D. (2020). Genotypic and environmental effects on wheat technological and nutritional quality, In: Igrejas, G., Ikeda, T. M. and Guzmán C. (Eds), Wheat quality for improving processing and human health, Springer, pp. 171-204, https://doi.org/10.1007/978-3-030-34163-3
Kaplan E. A., Pehlivan, A., Sanal, T., Salantur, A., Kilic, G., Dugan, G., Boyaci, I. H., & Koksel, H. (2020). Utilization potential of Glutograph in wheat breeding programs and the influence of genotype and environment on bread wheat quality. Cereal Chemistry, 97(3), 634–641. https://doi.org/10.1002/cche.10279
Kasahun, C., & Alemu, G. (2022). Evaluation of physical and chemical quality characteristics of elite bread wheat (Triticum aestivum L.) genotypes. International Journal of Nutrition and Food Sciences, 11(4), 102-109. https://doi.org/10.11648/j.ijnfs.20221104.12
Khazratkulova, S., Sharma, R. C., Amanov, A., Ziyadullaev, Z., Amanov, O., Alikulov, S., Ziyaev, Z. & Muzafarova, D. (2015). Genotype × environment interaction and stability of grain yield and selected quality traits in winter wheat in Central Asia. Turkish Journal of Agriculture and Forestry, 39, 920–929. https://doi.org/10.3906/tar-1501-24
Kiszonas, A. M., & Morris, C. F. (2018). Wheat breeding for quality: A historical review. Cereal Chemistry, 95, 17–34. https://doi.org/10.1094/CCHEM-05-17-0103-Fi
Kyratzis, A. C., Pallides, A., & Katsiotis, A. (2022). Investigating stability parameters for agronomic and quality traits of durum wheat grown under Mediterranean conditions. Agronomy, 12(8), 1774. https://doi.org/10.3390/agronomy12081774
Lemma, A. Z.., Mekbib F., Assefa K., & Bishow, Z. (2022). Estimation of genotype x environment interaction and stability analysis for protein content on durum wheat (Triticum turgidium Var Durum). African Journal of Agricultural Research, 18(3), 246-253. https://doi.org/10.5897/AJAR2021.15905
Ma, M., Li, Y., Xue, C., Xiong, W., Peng, Z., Han, X., Ju, H., and He, Y. (2021). Current situation and key parameters for improving wheat quality in China. Frontiers in Plant Science,12, 638525. https://doi.org/10.3389/fpls.2021.638525
Maich, R.H., Manera, G.A., Londero, W.H., Vanzetti, L. S., Helguera, M. & Di Rienzo, J. A. (2020). Changes in yield determinants and quality traits in wheat after 12 cycles of recurrent selection. Cereal Research Communications, 48, 533–538 https://doi.org/10.1007/s42976-020-00053-w
Malik, A. H., Kuktaite, R., & Johansson, E. (2013). Combined effect of genetic and environmental factors on the accumulation of proteins in the wheat grain and their relationship to bread-making quality. Journal of Cereal Science, 57(2), 170–174. https://doi.org/10.1016/j.jcs.2012.09.017
Marcheva, M. (2021). Yield and quality stability of common winter wheat varieties. Bulgarian Journal of Agricultural Science, 27(S. 1), 111–118. https://journal.agrojournal.org/page/en/details.php?article_id=3526
Mirosavljević, M., Momčilović, V., Živančev, D., Aćin, V., Jocković, B., Mikić, S., Takač, V., & Denčić, S. (2020). Genetic improvement of grain yield and bread-making quality of winter wheat over the past 90 years under the Pannonian Plain conditions. Euphytica, 216(12), 184. https://doi.org/10.1007/s10681-020-02724-5
Mohammadi, R., Sadeghzadeh, B., Ahmadi, M. & Amri, M. A. (2020). Biological interpretation of genotype × environment interaction in rainfed durum wheat. Cereal Research Communications 48, 547–554. https://doi.org/10.1007/s42976-020-00056-7
Mutwali, N. I. A., Mustafa, A. I., Gorafi, Y. S. Mohamed A., & Ahmed, I. A. (2015). Effect of environment and genotypes on the physicochemical quality of the grains of newly developed wheat inbred lines. Food Science & Amp; Nutrition, 4(4), 508–520. https://doi.org/10.1002/fsn3.313
Nankova, M., Doneva, S., Iliev, I., & Krustev, S. (2020). Soil organic fertilization in long-term low-input cropping system and its effect on the bread making flour properties determined by albumen-protein complex. Acta Scientific Agriculture, 4(7), 54-66.
Nazarenko, M., Mykolenko, S., & Okhmat, P. (2020). Variation in grain productivity and quality of modern winter wheat varieties in northern Ukrainian Steppe. Ukrainian Journal of Ecology, 10(4), 102–108. https://doi.org/10.15421/2020_175
Negash, G., & Birr, A. (2022). Adaptability Study of Yield and Yield Related Trait Performance of Improved Bread Wheat (Triticum aestivum L.) Varieties in North Shewa Zone Oromia, Ethiopia. South Asian Research Journal of Agriculture and Fisheries 4(6), 77-84. https://doi.org/10.36346/sarjaf.2022.v04i06.001
Nehe, A., Akin, B., Sanal, T., Evlice, A.K., U.R., Dincer, N., Unsal, R., Dinçer, N., , Lütfü, D., Hatice G., Sevim, I., Şinasi, O., Yaktubay, S., , Ezici A., Guzman, C., & Morgounov, A. (2019) Genotype x environment interaction and genetic gain for grain yield and grain quality traits in Turkish spring wheat released between 1964 and 2010. PLoS ONE 14(7), e0219432. https://doi.org/10.1371/journal.pone.0219432
Oikonomou, N. A., Bakalis, S., Rahman, M. S. &. Krokida, M. K. (2015). Gluten index for wheat products: main variables in affecting the value and nonlinear regression model. International Journal of Food Properties, 18(1), 1-11, https://doi.org/10.1080/10942912.2013.772198
Öztürk, İ., & Korkut, K. Z. (2020). Stability of quality parameters of bread wheat (Triticum aestivum L.) genotypes under drought stress condition. Ekin Journal of Crop Breeding and Genetics, 6(1):27-37.
Pacheco, Á., Vargas, M., Alvarado, G., Rodríguez, F., Crossa, J. & Burgueño, J. (2015). GEA-R (Genotype x Environment Analysis with R for Windows) Version 4.1, CIMMYT Research Data & Software Repository Network, V.16, https://hdl.handle.net/11529/10203
Panayotova, G., Kostadinova , S., Stefanova-Dobreva, S., & Muhova, A. (2021). Influence of long-term fertilization and environments on test weight of durum wheat (Triticum durum Desf.) grain. Agricultural Science and Technology, 13(1), 52-56. https://doi.org/10.15547/ast.2021.01.010
Penchev, E., Doneva, S., & Alexandrova, R. (2019). Investigation on the adaptability of a group of topical common winter wheat cultivars. International Journal of Innovative Approaches in Agricultural Research, 3(2), 169–176. https://doi.org/10.29329/ijiaar.2019.194.2
Plavšin, I., Gunjaˇca, J., Šimek, R., & Novoselovi´c, D. (2021) Capturing GEI patterns for quality traits in biparental wheat populations. Agronomy, 11, 1022. https://doi.org/10.3390/agronomy11061022
Pour-Aboughadareh, A., Barati, A., Koohkan, S. A., Jabari, M., Marzoghian, A., Gholipoor, A., Shahbazi-Homonloo, K., Zali, H., Poodineh, O. & Kheirgo, M. (2022). Dissection of genotype-by-environment interaction and yield stability analysis in barley using AMMI model and stability statistics. Bulletin of the National Research Centre, 46, 19. https://doi.org/10.1186/s42269-022-00703-5
Quintero, A., Molero, G., Reynolds, M. P., & Calderini, D. F. (2018). Trade-off between grain weight and grain number in wheat depends on GxE interaction: A case study of an elite CIMMYT panel (CIMCOG). European Journal of Agronomy, 92, 17–29. https://doi.org/10.1016/j.eja.2017.09.007
Rakszegi, M., Mikó, P., Löschenberger, F., Hiltbrunner, J., Aebi, R., Knapp, S., Tremmel-Bede, K., Megyeri, M., Kovács, G., Molnár-Láng, M., Vida, G., Láng, L., & Bedő, Z. (2016). Comparison of quality parameters of wheat varieties with different breeding origin under organic and low-input conventional conditions. Journal of Cereal Science, 69, 297–305. https://doi.org/10.1016/j.jcs.2016.04.006
Roostaei, M., Jafarzadeh, J., Roohi, E., Nazary, H., Rajabi, R., Mohammadi, R., Khalilzadeh, G. R., Seif, F., Mohammad, S., Mirfatah, M., Amiri, S. S., Hatamzadeh, H., & Ahmadi, M. (2022). Genotype × environment interaction and stability analyses of grain yield in rainfed winter bread wheat. Experimental Agriculture, 58, e37. https://doi.org/10.1017/S0014479722000345
Rozbicki, J., Ceglińska, A., Gozdowski, D., Jakubczak, M., Cacak-Pietrzak, G., Mądry, W., Golba, J., Piechociński, M., Sobczyński, G., Studnicki, M., & Drzazga, T. (2015). Influence of the cultivar, environment and management on the grain yield and bread-making quality in winter wheat. Journal of Cereal Science, 61, 126–132. https://doi.org/10.1016/j.jcs.2014.11.001
Saltz, J. B., Bell, A. M., Flint, J., Gomulkiewicz, R., Hughes, K. A., & Keagy, J. (2018). Why does the magnitude of genotype‐by‐environment interaction vary? Ecology and Evolution, 8(12), 6342–6353. https://doi.org/10.1002/ece3.4128
Sanchez-Garcia, M., Álvaro, F., Martín-Sánchez, J. A., Sillero, J. C., Escribano, J., & Royo, C. (2012). Breeding effects on the genotype×environment interaction for yield of bread wheat grown in Spain during the 20th century. Field Crops Research, 126, 79–86. https://doi.org/10.1016/j.fcr.2011.10.001
Schneider, H. M. (2022). Characterization, costs, cues and future perspectives of phenotypic plasticity-Review. Annals of Botany, 130(2), 131–148, https://doi.org/10.1093/aob/mcac087
Schuler, S. F., Bacon, R. K., Finney, P. L., & Gbur, E. E. (1995). Relationship of test weight and kernel properties to milling and baking quality in soft red winter wheat. Crop Science, 35(4), 949–953. https://doi.org/10.2135/cropsci1995.0011183x003500040001x
Sekularac, A., Torbica, A., Zivancev, D., Tomic, J., & Knezevic, D. (2018). The influence of wheat genotype and environmental factors on gluten index and the possibility of its use as bread quality predictor. Genetika, 50(1), 85–93. https://doi.org/10.2298/gensr1801085s
Stoeva, I. (2012). Technological evaluation of new common winter wheat lines developed at Dobrudzha Agricultural Institute - General Toshevo. Agricultural Science and Technology, 4(1), 10–14.
Stoyanov, H. (2020). Analysis on test weight of Bulgarian triticale cultivars. Rastenievadni nauki, 57(6) 3-16.
Surma, M., Adamski, T., Banaszak, Zofia, Kaczmarek, Z., Kuczyńska, H., Majcher, M., Ługowska, B., Obuchowskił, W., Salmanowicz, B., & Krystkowiak K. (2012). Effect of genotype, environment and their interaction on quality parameters of wheat breeding lines of diverse grain hardness, Plant Production Science, 15(3), 192-203, https://doi.org/10.1626/pps.15.192
Taneva, K., Bozhanova, V., & Dragov, R. (2019a). Assessment of Diversity and Association between Agronomic and Quality Traits in an Assortiment of Durum Wheat Genotypes. International Journal of Innovative Approaches in Agricultural Research, 3(3), 480–490. https://doi.org/10.29329/ijiaar.2019.206.13
Taneva, K., Bozhanova, V., & Petrova, I. (2019b). Variability, heritability and genetic advance of some grain quality traits and grain yield in durum wheat genotypes, Bulgarian Journal of Agricultural Science, 25(2), 288-295.
Tayyar, Ş. (2010). Variation in grain yield and quality of Romanian bread wheat varieties compared to local varieties in North-western Turkey. Romanian Biotechnological Letters, 15(2), 5789-5796.
Torbica, A., Antov, M., Mastilovic, J., & Kneževic, D. (2007). The influence of changes in gluten complex structure on technological quality of wheat (Triticum aestivum L.). Food Research International, 40, 1038–1045. www.elsevier.com/locate/foodres
Tsenov, N., Gubatov, T., & Yanchev, I. (2020). Correlations between grain yield and related traits in winter wheat under multi-environmental traits. Agricultural Science and Technology 12(4), 295-300. https://doi.org /10.15547/ast.2020.04.047
Tsenov, N., Gubatov, T., & Yanchev, I. (2021). Correlations between quantitative traits of winter common wheat - breeding tool for increasing grain yield. Agricultural Science and Technology, 13(2), 119-128. https://doi.org/10.15547/ast.2021.02.020
Tsenov, N., Gubatov, T., & Yanchev, I. (2022a). Ecological and biological explanation of genotype x environment interaction for common winter wheat (Triticum aestivum L.). Bulgarian Journal of Crop Science, 59(4), 28-42.
Tsenov, N., Gubatov, T., & Yanchev, I. (2022b). Comparison of statistical parameters for estimating the yield and stability of winter common wheat. Agricultural Science and Technology, 14(3), 10-25. https://doi.org/10.15547/ast.2022.03.032
Tsenov, N., Gubatov, T., & Yanchev, I. (2022c). Evaluation of heritability and genetic advance of selection for grain quality in common wheat (Triticum aestivum L.). Agricultural Science and Technology, 14(2), 12-26. https://doi.org/10.15547/ast.2022.02.015
Tsenov, N., Stoeva, I., & Tsenova, E. (2014). Study of opportunities for effective use of varieties from Ukraine for creating early winter wheat lines II. Quality of Grain. Agricultural Science and Technology, 6(2), 156-161.
Uhr, Zl., Angelova, T., Dimitrov E., & Dragov, R. (2022). Evaluation of milling traits of common winter wheat and their stability for the region of Sadovo. Journal of Mountain Agriculture on the Balkans, 25 (3), 119-132.
Vida, G., Szunics, L., Veisz, O., Bedő, Z., Láng, L., Árendás, T., Bónis, P., & Rakszegi, M. (2014). Effect of genotypic, meteorological and agronomic factors on the gluten index of winter durum wheat. Euphytica, 197(1), 61–71. https://doi.org/10.1007/s10681-013-1052-6
Vida, G., Szunics, L., Veisz, O., & Cséplő, M. (2022). Breeding for improved gluten strength and yellow pigment content in winter durum wheat. Bulgarian Journal of Crop Science, 59(6) 16-3.
Wieser, H., Koehler, P., & Scherf, K. A. (2020). The two faces of wheat, Frontiers in nutrition, 7, 517313. https://doi.org/10.3389/fnut.2020.517313
Williams, R. M., O’Brien, L., Eagles, H. A., Solah, V. A., & Jayasena, V. (2008). The influences of genotype, environment, and genotype × environment interaction on wheat quality. Australian journal of agricultural research, 59(2), 95-111. https://www.publish.csiro.au/journals/ajar
Yabwalo, D. N., Berzonsky, W. A., Brabec, D., Pearson, T., Glover, K. D., & Kleinjan, J. L. (2018). Impact of grain morphology and the genotype by environment interactions on test weight of spring and winter wheat (Triticum aestivum L.). Euphytica, 214(7), 1-16. https://doi.org/10.1007/s10681-018-2202-7
Yan, W. (2021). A Systematic Narration of Some Key Concepts and Procedures in Plant Breeding. Frontiers in Plant Science 12, 724517. https://doi.org/10.3389/fpls.2021.724517
Yan, W., & Hunt L. (2001). Interpretation of genotype × environment interaction for winter wheat yield in Ontario. Crop Science, 41(1), 19–25. https://doi.org/10.2135/cropsci2001.41119x
Yashavanthakumar, K. J., Baviskar, V. S., Navathe, S., Patil, R. M., Bagwan, J. H., Bankar, D. N., & Singh, G. P. (2021). Impact of heat and drought stress on phenological development and yield in bread wheat. Plant Physiology Reports, 26(2), 357-367. https://doi.org/10.1007/s40502-021-00586-0
Zečević, V., Knežević, D., Bošković, J., & Madić, M. (2009). Effect of genotype and environment on wheat quality. Genetika, 41(3), 247-253.
Zhang, S., Shuliang, L., Li, Shen, Shujuan, C., Li, H., & Aiping, L. (2022). Application of near-infrared spectroscopy for the non-destructive analysis of wheat flour: A review. Current Research in Food Science 5, 1305–1312. https://doi.org/10.1016/j.crfs.2022.08.006
Živančev, D., Mirosavljević, M., Aćin, V., Momčilović, V., Mikić, S., Torbica, A., & Jocković, B. (2021). Variation in quality traits of newly developed Serbian wheat cultivars under different environmental conditions of Pannonian plain. Italian Journal of Agronomy, 17(1). https://doi.org/10.4081/ija.2021.1911
Downloads
Published
License
Copyright (c) 2023 Bulgarian Journal of Crop Science

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

