Structural fiber components of cell walls after herbicide treatment of bird's foot trefoil stand

Authors

DOI:

https://doi.org/10.61308/BVVU3973

Keywords:

bird's foot trefoil, herbicide treatment, fiber components, nutritional value, correlations, regressions

Abstract

In the period 2020 – 2023, in the experimental field of Research Institute of Mountain Stockbreeding and Agriculture – Troyan, on light grey pseudopodzolic soil, a scientific experiment with bird's-foot trefoil (Lotus corniculatus L.), variety Targovishte 1, was conducted with the following treatments: 1. Control – untreated; 2. Kalam (20 g/da); 3. Kalam (40 g/da); 4. Focus ultra (100 ml/da); 5. Focus ultra - (200 ml/da) was conducted. The aim was to determine the structural fiber components of the cell walls and to calculate the nutritive value of the forage as a function of herbicide treatment. It was found that herbicides applied in the 2 - 4 leaf stage of bird's foot trefoil did not significantly affect the fiber composition of cell walls and the nutritional value of forage. Relatively favorable in terms of forage quality stood out those treated with Kalam herbicide at 20 g/da, due to the lowest values obtained for neutral detergent fiber (NDF), acid detergent fiber (ADF) and cellulose, and the highest for hemicellulose, as well as the highest feed units for growth. Correlation analysis revealed the highest correlations between dry matter yield and the relative proportion of leaves (r = 0.833); between the relative proportion of stems in the stand and acid detergent lignin content (r = 0.829) and degree of lignification (r = 0.837); and between neutral detergent and acid detergent fiber (r = 0.940), allowing regression equations to be derived for predicting forage quality.

References

Arzani, H., Torkan, J., Jafari, M. & Nikkhah, A. (2001). Investigation on effects of phenological stages and environmental factors (soil and climate) on forage quality of some important range species. Journal of Agricultural Sciences, 32, 385 - 397.

Ayala, W. & Carambula, M. (2009). The agronomic value of the genus Lotus. Uruguay: INIA, 424 (Es).

Bozhanska, T., Naydenova, G. & Naydenova, Y. (2015). Study on grazing ecotype of birds foot trefoil in terms of selection. Plant science, LII(5), 17 - 22.

Chourkova, B. (2013). Agro-ecological aspects of the weed infestation control in birds foot trefoil grown for forage. Banat’s Journal of Biotechnology, IV(7), 29 - 34. DOI: https://doi.org/10.7904/2068-4738-IV(7)-29

Cohen, D. C. (2001). Degradability of crude protein from clover herbages used in irrigated dairy production systems in northern Victoria. Australian Journal of Agricultural Research, 52(3), 415 - 425. DOI: https://doi.org/10.1071/AR00033

Ducati, C., Neres, M. A., Castagnara, D. D., Oliveira, P. S. R., Weirich, D., Silva, G. M. & Sunahara, S. M. M. (2015). Forage potential of oat Iapar 61 alone or in association with birds foot trefoil. Cien. Inv. Agr., 42(3), 341 - 351. DOI: https://doi.org/10.7764/S0718-16202015000300003

Formoso, F. (2011). Production of seeds of forage species. Uruguay: INIA, 234 (Es).

Marinov-Serafimov, P., Naydenova, Y., Golubinova, I., Enchev, S., Kikindonov, Tz. & Ilieva, A. (2016). Influence of some herbicides on forage quality of alfalfa. Bulgarian Journal of Crop Science, 53(5-6), 67 - 75.

Naydenova, G., Hristova, Ts. & Aleksiev, Y. (2013). Objectives and approaches in the breeding of perennial legumes for use in temporary pasturelands. Biotechnology in Animal Husbandry, 29(2), 233 - 250. DOI: https://doi.org/10.2298/BAH1302233N

Naydenova, Y., Kyuchukova, A. & Pavlov, D. (2013). Plant cell walls fiber component analysis and digestibility of birds foot trefoil (Lotus corniculatus L.) in the vegetation. Agricultural Science and Technology, 5(2), 164 - 167.

Naydenova, Y., Vasilev, E. & Kirilov, A. (2015). Plant cell wall fiber components content and digestibility of orchard grass (Dactylis glomerata L.) and legume forage species in pure stands and mixtures. Journal of Mountain Agriculture on the Balkans, 18(1), 61 - 76.

Newman, Y. C., Adesogan, A. T., Vendramini, J. & Sollenberger, L. (2009). Defining Forage Quality: SS-AGR-322/AG332. EDIS, 5. https://edis.ifas.ufl.edu/pdffiles/AG/AG33200.pdf. DOI: https://doi.org/10.32473/edis-ag332-2009

Nikolova, I., Georgieva, N. & Naydenova, Y. (2016). Forage quality and energy feeding value estimation of alfalfa (Medicago sativa L.), treated by biological active compounds. Journal of Mountain Agriculture on the Balkans, 19(1), 78 - 95.

Niwińska, B., Strzetelski, J. A., Kowalczyk, J., Borowiec, F. & Domański, P. (2005). The effect of phenological stage and season on nutritive value, chemical composition and nutrient digestibility of lucerne (Medicago sativa L.) green forage in the alimentary tract of cattle. Czech Journal of Animal Science, 50(11), 511 - 518. DOI: https://doi.org/10.17221/4256-CJAS

Oliveira, Jr. R. S. & Inoue, M. H. (2011). Selectivity of herbicides for crops and dangerous plants. In: Biologia e manejo de plantas daninhas (Oliveira Jr., R.S., Consttantin, J. & Inoue, M.H., Eds.). Curitiba: Omnipax, 243 - 262 (Es).

Petkova, R. & Pavlov, D. (2008). Nutritional value of varieties of winter fodder under irrigation. Bulgarian Journal of Crop Science, 45, 364 - 367.

Radovic, J., Dinic, B. & Pudio, V. (2003). Productivity and quality of some bird’s foot trefoil (Lotus corniculatus L.) varieties. In: Proceedings of the 12th Symposiumof the European Grassland Federation, Pleven, Bulgaria, 26 – 28 May, 118 - 121.

Rodrigues-Costa, A. C. P., Martins, D., Costa, N. V., Campos, C. F., Martins, C. C., Pereira, M. R. R. & Silva, J. I. C. (2011). Selectivity of herbicides applied pre-emergence in forage grasses. Planta Daninha, 29(3), 625 - 633 (Es). DOI: https://doi.org/10.1590/S0100-83582011000300017

Soster, M. T. B., Scheffer-Basso, S. M., Dall’Agnol, M., Brustolin, R. & Fontaneli, R. S. (2004). Agronomic characterization of birdsfoot trefoil genotypes (Lotus corniculatus L.). Revista Brasileira de Zootecnia, 33, 1662 - 1671 (Pt). DOI: https://doi.org/10.1590/S1516-35982004000700004

Srinivasa Reddy, M. S., Chen, F., Shadle, G., Jackson, L., Aljoe, H. & Dixon, R. A. (2005). Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.). Proceedings of the National Academy of Sciences, 102(46), 16573 - 16578. DOI: https://doi.org/10.1073/pnas.0505749102

Todorov, N., Atanassov, A., Ilchev, A., Ganchev, G., Mihailova, G., Girginov, D., Penkov, D., Shindarska, Z., Naydenova, Y., Nedjalkov, K. & Tshobanova, S. (2010). Practice in Animal nutrition. East-West Edition, Sofia, Bulgaria. ISBN 978-954-321-733-5.

Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583 - 3597. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Downloads

Published

29.10.2026

How to Cite

Churkova, K., & Churkova, B. (2026). Structural fiber components of cell walls after herbicide treatment of bird’s foot trefoil stand. Bulgarian Journal of Agricultural Science, 32(5), 1062-1067. https://doi.org/10.61308/BVVU3973