Investigation of antiphytoviral effects of thymol, eugenol, and 1,8-cineol essential oils on cucumber mosaic virus in Turkey
DOI:
https://doi.org/10.61308/tz159s38Keywords:
cucumber mosaic virus, antiphytoviral effect, plant virus, essential oilsAbstract
The exploration of natural antiviral agents, such as essential oils, is a critical area of research, offering promising alternatives for sustainable plant disease management and contributing to global agricultural resilience. In this regard, the present study investigated the antiviral efficacy of thymol, eugenol, and 1,8-cineol essential oils (EOs) against cucumber mosaic virus (CMV) in vivo. It focused on assessing the capability of these EOs, particularly at a concentration of 500 ppm, in both prophylactic and therapeutic contexts for plant health, particularly when applied prior to or concurrently with viral infection. The evaluation involved measuring a range of parameters, including local lesions of CMV on cucumber plants, symptom index, leaf width, plant height, and virus accumulation. Our findings demonstrated that thymol was significantly effective in mitigating CMV infection. Both eugenol and 1,8-cineole oils also showed efficacy in reducing local lesions when applied to host plants alongside the infecting virus. Based on our results, the essential oils exhibited substantial protective and curative effects against CMV, with the preventative (pre-inoculation) application proving more effective. In conclusion, this study highlights the potent antiviral activity of thymol, eugenol, and 1,8-cineole EOs against CMV, presenting them as viable alternative strategies for combating viral diseases in plants. In addition, our findings not only advance our understanding of plant-virus interactions, but also pave the way for more eco-friendly and effective agricultural practices, crucial in the face of escalating plant disease challenges worldwide.
References
Aktaş, M. & Akdura, N. (2023). Detection of Cucumber Mosaic Virus Method in Cucurbits Produced in Hakkari Using DAS-ELISA Method. Sinop University Journal of Natural Science, 8(2), 245 - 257. DOI: https://doi.org/10.33484/sinopfbd.1381790
Arshad, Z., Hanif, M. A., Qadri, R. W. K. & Khan, M. (2014). Role of essential oils in plant diseases protection: A review. International Journal of Chemical and Biochemical Sciences, 6, 11 - 17.
Batish, D., Singh, H., Kohli, R. & Kaur, S. (2008). Eucalyptus essential oil as a natural pesticide. Forest Ecology Management, 13(12), 2166 - 2174. DOI: https://doi.org/10.1016/j.foreco.2008.08.008
Bezi´c, N., Vuko, E., Dunkic, V., Rušˇci´c, M., Blaževi´c, I. & Burˇcul, F. (2011). Antiphytoviral activity of sesquiterpene-rich essential oils from four Croatian Teucrium species. Molecules, 16(9), 8119 - 8129. DOI: https://doi.org/10.3390/molecules16098119
Bezi´c, N., Dunkic, V. & Vuko, E. (2013). Antiphytoviral activity of essential oils of some Lamiaceae species and there most important compounds on CMV and TMV. In: Microbial Pathogens and Strategies for Combating Them: Science, Technology and Education; Méndez-Vilas, A., Ed. Formatex: Badajoz, Spain, 982 - 987.
Chen, J., Yan, X. H., Dong, J.-H., Sang, P., Fang, X., Di, Y. T., Zang Z.-K. & Hao, X.-J. (2009). Tobacco mosaic virus (TMV) inhibitors from Picrasma quassioides Benn. Journal of Agricultural and Food Chemistry, 57(15), 6590 - 659. DOI: https://doi.org/10.1021/jf901632j
Clark, M. F. & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34(3), 475 - 483. DOI: https://doi.org/10.1099/0022-1317-34-3-475
Dunkic, V., Bezić, N., Vuko, E. & Cukrov, D. (2010). Antiphytoviral Activity of Satureja montana L. ssp. variegata (Host) P. W. Ball Essential Oil and Phenol Compounds on CMV and TMV. Molecules, 15(10), 6713 - 6721. DOI: https://doi.org/10.3390/molecules15106713
Dunkic, V., Bezić, N. & Vuko, E. (2011). Antiphytoviral Activity of Essential Oil from Endemic Species Teucrium arduini. Natural Product Communications, 6(9), 1385 - 1388. DOI: https://doi.org/10.1177/1934578X1100600940
Dunkic, V., Vuko, E., Bezic´, N., Kremer, D. & Rusˇcˇic´, M. (2013). Composition and Antiviral Activity of the Essential Oils of Eryngium alpinum and E. Amethystinum. Chemıstry & Bıodıversıty, 10(10), 1894 - 1902. DOI: https://doi.org/10.1002/cbdv.201300061
Fauquet, C. M., Mayo, M. A., Maniloff, J., Desselberger, U. & Ball, L. A. (Eds.). (2005). Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego, 1162.
Francki, R. I. B., Mossop, D. W. & Hatta, T. (1979). Cucumber mosaic virus. CMI/AAB Description Viruses, 213, 6.
Helal, I. M. (2019). Use of biocides for controlling viral diseases that attack common bean and cucumber plants. Folia Horticulturae, 31(1), 159 - 170. DOI: https://doi.org/10.2478/fhort-2019-0011
Hoch, C. C., Petry, J., Griesbaum, L., Weiser, T., Werner, K., Ploch, M., Vershoor, A., Multhoff, G., Dezfouli, A. B. & Wollenberg, B. (2023). 1, 8-cineole (eucalyptol): A versatile phytochemical with therapeutic applications across multiple diseases. Biomedicine & Pharmacotherapy, 167, 115467. DOI: https://doi.org/10.1016/j.biopha.2023.115467
Islam, W., Zaynab, M., Qasim, M. & Wu, Z. (2017). Plant-virus interaction disease resistance in Focus. Hosts Viruses, 4(1), 5 - 20.
Karakus, S. (2024). Enhancing Post-Harvest Resilience: Investigating the Synergistic Effects of Essential Oil Combinations on Biochemical Profiles in Botrytis cinerea-Infected Apples. Horticulturae, 10(4), 341. DOI: https://doi.org/10.3390/horticulturae10040341
Karpinski, T. M. (2020). Essential Oils of Lamiaceae Family Plants as Antifungals. Biomolecules, 10(1), 103. DOI: https://doi.org/10.3390/biom10010103
Kaya, O., Karakus, S., Bozkurt, A., Canturk, S., Yilmaz, T. & Hatterman-Valenti, H. (2025). Essential oil compounds and their impact on grape (Vitis vinifera L. cv. Narince) physiology under Botrytis cinerea infection. Physiological and Molecular Plant Pathology, 136, 102544. DOI: https://doi.org/10.1016/j.pmpp.2024.102544
Kowalczyk, A., Przychodna, M., Sopata, S., Bodalska, A. & Fecka, I. (2020). Thymol and thyme essential oil - new insights into selected therapeutic applications. Molecules, 25(18), 4125. DOI: https://doi.org/10.3390/molecules25184125
Lecoq, H. & Katis, N. (2014). Control of cucurbit viruses. Advances in Virus Research, 90, 255 - 296. DOI: https://doi.org/10.1016/B978-0-12-801246-8.00005-6
Lopez-Reyes, J. G., Spadaro, D., Gollino, M. L. and Garibaldi, A. (2010). Efficacy of plant essential oils on postharvest control of rot caused by fungi on four cultivars of apples in vivo. Flavour and Fragrance Journal, 25(3), 171 - 177. DOI: https://doi.org/10.1002/ffj.1989
Negai, A., Duarte, L. M. L. & Santos, D. Y. A. C. (2011). Influence of viral infection on essential oil composition of Ocimum basilicum (Lamiaceae). Natural Product Communications, 6(8), 1189 - 1192. DOI: https://doi.org/10.1177/1934578X1100600836
Othman, B. A. & Shoman, S. A. (2004). Antiphytoviral activity of the Plectranthus tenuiflorus on some important viruses. International Journal of Agriculture and Biology, 6(5), 844 - 849.
Paduch-Cichal, E., Mirzwa-Mróz, E., Wojciechowska, P., Ba˛czek, K., Kosakowska, O., Weglarz, Z. & Szyndel, M. S. (2023). Antiviral activity of selected essential oils against cucumber mosaic virus. Plants, 12, 18. DOI: https://doi.org/10.3390/plants12010018
Petrov, N., Stoyanova, M. & Valkova, M. (2016). Antiviral activity of plant extract from Tanacetum vulgare against Cucumber mosaic virüs and Potato virus. Journal of Bioscience and Biotechnology, 5(2), 189 - 194.
Pitarokili, D., Tzakou, O., Loukis, A. & Harvala, C. (2003). Volatile metabolites from Salvia fruticosa as antifungal agents in soil-borne pathogens. Journal of Agricultural and Food Chemistry, 51(11), 3294 - 3301. DOI: https://doi.org/10.1021/jf0211534
Raccah, B., Galon, A. & Eastop, V. F. (1985). The role of flying aphid vectors in the transmission of cucumber mosaic virus and potato virus Y to peppers in Israel. Annals of Applied Biology, 106, 451 - 460. DOI: https://doi.org/10.1111/j.1744-7348.1985.tb03135.x
Rao, A., Zhang, Y., Muend, S. & Rao, R. (2010). Mechanism of antifungal activity of terpenoid phenols resembles calcium stress and inhibition of the TOR pathway. Antimicrobial Agents and Chemotherapy, 54, 5062 - 5069. DOI: https://doi.org/10.1128/AAC.01050-10
Ruščić, M., Vuko, E., Bezić, N., Kremer, D. & Dunkic, V. (2017). Croatian Micromeria fruticulosa – Essential Oil Composition and Antiphytoviral Activity. Natural Product Communications, 12(7), 1133 - 1135. DOI: https://doi.org/10.1177/1934578X1701200733
Salehi, B., Mishra, A. P., Shukla, I., Sharifi-Rad, M., Contreras, M. D., Segura Carretero, A., Fathi, H., Nasrabadi, N. N., Kobarfard, F. & Sharifi-Rad, J. (2018). Thymol, thyme, and other plant sources: Health and potential uses. Phytotherapy Research, 32(9), 1688 - 1706. DOI: https://doi.org/10.1002/ptr.6109
Strange, R. N. & Scott, P. R. (2005). Plant Disease: A Threat to Global Food Security. Annual Review of Phytopathology, 43(1), 83 - 116. DOI: https://doi.org/10.1146/annurev.phyto.43.113004.133839
Taglienti, A., Donati, L., Ferretti, L., Tomassoli, L., Sapienza, F., Sabatino, M., Di Massimo, G., Fiorentino, S., Vecchiarelli, V. & Nota, P. (2022). In vivo antiphytoviral activity of essential oils and hydrosols from Origanum vulgare, Thymus vulgaris, and Rosmarinus officinalis to control Zucchini Yellow Mosaic Virus and Tomato Leaf Curl New Delhi Virus in Cucurbita pepo L. Frontiers in Microbiology, 13, 840893. DOI: https://doi.org/10.3389/fmicb.2022.840893
Takikawa, A., Keiko, A., Yamamoto, M., Ishımaru, S., Yasui, M., Okubo, Y. & Yokoigawa, K. (2002). Antimicrobial activity of nutmeg against Escherichia coli 0157. Journal of Bioscience and Bioengineering, 94(4), 315 - 320. DOI: https://doi.org/10.1016/S1389-1723(02)80170-0
Ulanowska, M. & Olas, B. (2021). Biological properties and prospects for the application of eugenol - a review. International journal of molecular sciences, 22(7), 3671. DOI: https://doi.org/10.3390/ijms22073671
Valero, M. & Salmeron, M. C. (2003). Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. International Journal of Food Microbiology, 85, 73 - 81. DOI: https://doi.org/10.1016/S0168-1605(02)00484-1
Vuko, E., Dunkic, V., Bezi´c, N., Rušˇci´c, M. & Kremer, D. (2012). Chemical composition and antiphytoviral activity of essential oil of Micromeria graeca. Natural Product Communications, 7(9), 1227 - 1230. DOI: https://doi.org/10.1177/1934578X1200700933
Wink, M. (2003). Evolution of secondary metabolites from an ecological and molecular phylogenetic perspective. Phytochemistry, 64(1), 3 - 19. DOI: https://doi.org/10.1016/S0031-9422(03)00300-5
Yardımcı, N. & Eryiğit, H. (Çulal-Kılıç). (2006). Identification of Cucumber Mosaic Virus in Tomato (Lycopersicon esculentum) growing areas in the North-West Meditarrenean Region of Turkey. New Zealand Journal of Crop and Horticultural Science, 34(2), 173-175. DOI: https://doi.org/10.1080/01140671.2006.9514403
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