Application of mobile fluorescence spectroscopy as a method for the analysis of accessions of different varieties of radishes (Raphanus sativus L.) during the growing season
DOI:
https://doi.org/10.61308/5bj2an40Keywords:
radish accessions (Raphanus sativus), growing season, varietal differences, uncontrolled conditions, field method, fluorescence spectroscopyAbstract
The study aims to establish the application of fluorescence spectroscopy as a field method for determining varietal differences in radish accessions (Raphanus sativus) during the growing season. The proposed method includes studies of radish leaves from standard varieties and those from first-generation hybrids by means of fluorescence spectroscopy. They will be compared to determine the spectral distribution due to the varietal differences of a given genotype. Specimens were grown under uncontrolled field conditions. This will allow the approach to be practiced non-invasively in the quality control of radish production on undefined premises and outdoors. The experimental studies were carried out locally on the farm. The spectral installation for generating emission fluorescence spectra is mobile. In its setup (optical setup), a system engineering approach based on the classical principles of modern optoelectronics was applied. The results of the experiment can be used to optimize the time for the analysis of the varietal differences of radish genotypes during the growing season under uncontrolled conditions. The stability of the breeding variety and its common blacks with an established variety of the same species can be observed by monitoring the intensity of the signal. This will support the process of determining the belonging of a specific variety to a given variety, even for samples of unknown origin, when it is necessary to qualify the result of the samples in a short time.
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