ПРОУЧВАНЕ НА ЕФЕКТА НА АФЛАТОКСИН В1 ВЪРХУ ХЕМАТОЛОГИЧНИТЕ ПОКАЗАТЕЛИ ПРИ ПАТЕТА МЮЛАРИ
Ключови думи :
Aflatoxin B1, haematological changes, mallard duclings Mycotox NgАбстракт
In this experiment, the toxic effects of AFB1 on some haematological parameters were investigated in mulard ducks, namely red blood cell counts (RBC), haemoglobin content (HGB), haematocrit (HCT), platelet counts (PLT), white blood cell counts (WBC), differential white blood cell counts (WBC %), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). Experiments were conducted with 4 groups of 20 10-day-old mulard ducks each. The groups were as followed: group I – control, fed a standard compound feed according to the species and the age; group II – experimental, whose feed was supplemented with 0.5 mg/kg AFB1 , group III – experimental, supplemented with 0.8 mg/kg AFB1 and group IV – experimental, supplemented with 0.5 mg/kg AFB1 and 2 g/kg Mycotox NG. The duration of the experiment was 42 days. Haematological analysis performed on the 21st day
of the trial showed reduction in RBC, HCT, HGB and PLT and increased total WBC in groups II and III. The percentages of the different leukocyte classes (differential leukocyte counts) demonstrated increased proportion of heterophils and lower percentages of lymphocytes. The observed changed tended to become more pronounced on the 42nd day of the experiment.
There were no statistically significant changes in MCV, MCH, and MCHC, as well as in eosinophil, basophil, and monocyte percentages between control and treated groups (р>0.05). The supplementation of the feed with mycosorbent – Mycotox NG (experimental group IV) reduced partly the harmful effect of AFB1 on studied haematological indices.
Литература (библиография)
Ангелов А., Н. Ибришимов и С. Милашки, 1999. Клинико-лабораторни изследвания във ветеринарната медицина. “Проф. Марин Дринов”, София.
Наредба №44/20.04.2006г. за ветеринарномедицинските изисквания към животновъдните обекти ДВ 41 Приложение № 9/ІІ, стр.61-62.
Abdel-Wahhab, M. A., S. A. Nada, F. A. Khalil, 2002. “Physiological and toxicological responses in rats fed aflatoxincontaminated diet with or without sorbent materials,” Animal Feed Science and Technology, 97, 3-4, 209–219.
Al-Daraji, H. J., I. A. Al-Ani, J. K. Minati. H. E. AlHiti, 2004. The use of different methods to suppress the effect of aflatoxicosis and its influence on certain blood traits in broiler. Iraqi Journal of Agricultural Science, 35, 2, 103-112.
Aravind, K. L., V. S. Patil, G. Dewegowda, B. Umakantha, S. P. Ganpule, 2003: Efficacy of esterified glucomannan to counteract mycotoxicosis in naturally contamined feed on performance and serum biochemical and hematological parameters in broilers. Poultry Science, 82, 4, 571-576.
Aved, I. A. M., R. Dafella, A. F. Yogi, S. E. I. Adam, 1991. Effect of ochratoxin A on Lohaman type chicks. Veterinary and Human Toxicology, 33, 357-360.
Basmacioglu, H., H. Oguz, M. Ergul, R. Зol, Y. O. Birdane, 2005. Effect of dietary esterified glucomannan on performance, serum biochemistry and haematology in broilers exposed to aflatoxin. Czech Journal of Animal Science, 50, 1, 31-39.
Bintvihok, A., S. Thiengnin, K. Doi K, S. Kumagai, 2002. Residues of Aflatoxins in the Liver, muscle and Eggs of Domestic Fowls. Journal of Veteterinary Medical Science, 64, 11,
Campbell, M. L., J. D. May, W. E. Huff, J. A. Doevr, 1983. Evaluation of immunity of young broiler chickens during aflatoxicosis and ochratoxicosis. Poultry Science, 62, 2138-2144.
Cheeke, P. R., 1998. Natural toxicants in Feed, forages and Poisonous Plants. 2nd ed. Interstate publishers, Danville, IL
Dakovic, A., M. Tomasevic-Canovic, V. Dondur, G. E. Rottinghaus, V. Medakovic, S. Zaric, 2005. Adsorption of mycotoxins by organozeolites. Colloids and Surfaces B Biointerfaces, 46, 20-25.
Dalvi, R. R., 1986. An overview of aflatoxicosis of poultry: its characteristics, prevention and reduction. Veterinary Reserch Communications, 10, 429–443.
Dietert, R. R., S. E. Bloom, M. A. Qureshi, U. C. Nanna, 1983. Hematological Toxicology following Embryonic Exposure to Aflatoxin-B1. Experimental Biology and Medicine, 173, 4, 481-485.
Directive 2002/32/EC of the European parliament and of the council of 7 may 2002 on undesirable substances and products in animal feed. Official Journal, No., L 140, 30, 5, 2002.
Doerr, J. A., R. B. Huff, 1980. Interactive effects of aflatoxin and ochratoxin A on some blood constituents in broiler chickens. Poultry Science, 59:1600-1602.
Donmez, N., H. H. Dönmez E. Keskin, Э. Kэsadere, 2012. Research ArticleEffects of Aflatoxin on Some Haematological Parameters and Protective Effectiveness of Esterified Glucomannan in Merino Rams. The Scientific World Journal, 10.1100/2012/342468, 1-4.
El-Bouhy, Z. M., A. A. Ali, M. S. Helmy, 1993. “Preliminary studies on aflatoxicosis of contaminated food”. Zagazig Veterinary Journal 21, 4, 607-624.
EL-Lethey, H.. H. El-Zorb, 2004. Effect of dimetyl diphenil bicarbohylate (DOB) on roilers during experimental aflatoxicosis. In: Proceedings 1rst Annimal Conference, Faculty Veterynary Medicine, Moshtohor, Sept, 2004, 215-233.
Eraslan, G., D. Essiz, M. Akdogan, F. Sahindokuyucu, L. Altintas, 2005. The Effects of Aflatoxin and Sodium Bentonite Combined and Alone on Some lood Electrolyte Levels in Broiler Chickens. Turkish Journal of Veterinary & Animal Sciences, 29, 3, 601-605.
Fonseca. H., 2003. Legislação sobre micotoxinas www.micotoxinas.com.br.
Girish, C. K., G. Devegowda, 2006. Efficacy of Glucomannan-containing yeast product (Mycosorb®) and hydrated sodium calcium aluminosilicate in preventing the individual and combined toxicity of aflatoxin and T-2 toxin in commercial broilers. Asian-Australian Journal of Animal Science, 19, 6, 877-883.
Halliwell, B., 2007. Oxidative stress and cancer: have we moved forward? Biochemistry Journal, 401, 1-11.
Han, X. Y., Q. C.Huang, W. E. Li, Z. R. Xu, 2008. Changes in growth performance, digestive enzyme activities and nutrient digestibility of cherry valley ducks in response to aflatoxin B1 levels. Livestock Science, 119,:216–220.
Harvey, R. B., L. F. Kubena, T. D. Phillips, M. H. Elissalde, W. E. Huff, 1991. Diminution of aflatoxin toxicity to growing lambs by dietary supplementation with hydrated sodium calcium aluminosillicate. American Journal of Veterinary Research, 52, 152-156.
Hashmi, I., T. N. Pasha, M. A. Jabbar, M. Akram, A. S. Hashmi, 2006. Study of adsorption potential of yeast sludge against aflatoxins in broiler chicks. Journal of Animal and Plant Science, 16, 1-2, 2006.
Huff, W. E., L. F. Kubena, R. B. Harvey, W. M. Hagler, S. P. Sorenson, T. D. Philips, C. R. Giegler, 1986. Individual and combined effect of aflatoxin and deoxynivalenol (DON, Vomitoxin) in broiler chicken. Poultry Science, 65, 7, 1291–1298.
Huff, W. E., R. B. Harvey, L. F. Kubena, G. E. Rottinghaus, 1988. Toxic synergism between aflatoxin and T-2 toxin in broiler chicken. Poultry Science, 67, 1418–1420
Hussein, H. S., J. M. Brasel, 2001. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology, 167, 2, 101–134.
Huwig, A., S. Freimund, O. Käppeli, H. Dultler, 2001. Mycotoxin detoxication of animal feed by different absorbents. Toxicology Letters, 122: 691-699.
International Agency for Research on Cancer (IARC), 2002. Summaries and evaluations: aflatoxins. Lyon: IARC Press, 82:171.
Kamei, K., A. Watanabe, 2005. Aspergillus mycotoxin and their effect on the host. Medical Mycology Supplement, 43: 95-99.
Kana, J. R., A. Teguia, B. M. Mungfu, J. Tchoumboue, 2011. Growth performance and carcass characteristics of broiler chickens fed diets supplemented with graded levels of charcoal from maize cob or seed of Canarium schweinfurthii Engl. Tropical Animal Health and Production, 43, 51-56.
Kececi, T., Q. Demet, H. Oguz, 1995. Single and combination effects of dietary aflatoxin and adsorbent (Mycofix Plus) on some hematological and serum biochemical parameters of broiler chickens Journal of Veterinary Science, 11, 95-101.
Kececi, T., H. Oguz, V. Kurtoglu, O. Demet, 1998. Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and hematological characters of broiler chickens during aflatoxicosis. British Poultri Science, 39, 3, 452-458.
Kennedy, D., A. Delaney, G. Koren, 1998. Mutagens, carcinogens and teratogens. In: Goldfrank L (ed) Goldfrank’s toxicologic emergencies, 6th edn. Appleton & Lange, Stanford, pp 262–273
Kubena, L., R. B. Harvey, T. D. Phillips, B. A. Clement, 1993. Effect Of Hydrated Sodium Calcium Aluminosilicates On Aflatoxicosis In Broiler Chicks. Poultry Science 72, 4, 651–657.
Kubena, L. F., R. B. Harvey, W. E. Huff, D. E. Corrier, T. D. Phillips, G. E. Rottinghaus, 1990. Efficacy of a hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and T-2 toxin. Poultry Science, 69, 7, 1078-1086.
Kurtzman, C..P., B. W. Horn, C. W. Hesseltine, 1987.Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie Leeuwenhoek, 53, 147-158
Lillehoj, E. B., 1983. “Effect of environmental and cultural factors of aflatoxin contamination of developing corn kernels.” In: V.L. Diener, R.L. Asquith and J.W. Dickens (Eds). Aflatoxin and A. flavus in corn. Southern Coop Serv. Bull. 279, Craftmaster, Opelika, Ala, 112P.
Mabbett, T., 2005. Integrated management of mycotoxins. Poult. Int., 44, 8, 10-14.
Mohamed, A. H., M. H. Mohamed, 2009. Haemato-Biochemical And Pathological Studies On Aflatoxicosis And Treatment Of Broiler Chicks In Egypt. Veterinaria Italiana, 45, 2, 323-37.
Mohiuddin, S. M., S. M. A. Warasi, M. V. Reddy, 1993. Haematological and biochemical changes in experimental ochratoxicosis in broiler chicken. Indian Veterinary Journal, 70, 613-617.
Oguz, H., 2012. Detoxification of aflatoxin in poultry feed: a review from experimental trials. Lohmann Information, 47, 2, 45-56.
Oguz, H., T. Kececi, Y. O. Birdane, F. Onder, V. Kurtoglu, 2000. Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during experimental aflatoxicosis. Research in Veterinary Science, 69, 1, 89-93.
Raju, M., G. Devegowda, 2000. Influence of esterifiedglucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin). British Poultry Science, 41,640–650.
Ramos, A. J. E., fink-Gremmels, E. Hernбndez, 1996. Prevention of toxic effects of mycotoxins by means of nonnutritive absorbent compounds. Journal of Food Protection, 59, 631-641.
Royes, J. B., R. P. Yanong, 2002. “Molds in fish feeds and aflatoxicosis.” Copyright by the University of Florida, Institute of Agricultural Science (UF/ IFAS).
Safameher, A., 2008. Effects of clinoptilolite on performance, biochemical parameters and hepatic lesions in broiler chickens during aflatoxicosis . Journal of Animal and Veterinary Advances, 7, 4, 381-388.
Sakhare, P. S., S. D. Harne, D. R. Kalorey, S. R. Warke, A. G. Bhandarkar, N. V. Kurkure, 2007. Effect of Toxiroak polyherbal feed supplement during induced aflatoxicosis, ochratoxicosis and combined mycotoxicoses in broilers. Veterinarski arhiv, 77, 2, 129-146.
Sawarkar, A, R, P. M. Sonkusale, N. V. Kurkure, C. R. Jangade, S. Maini, K. Ravikanth2011. Experimental afla & ochratoxininduced mixed mycotoxicosis in broiler and its amelioration withherbomineral toxin binder toxiroak gold. International Journal ofPoultry Science.10, 7, 560-566.
Singh, A., K. C. Satija, S. K. Mahipal, 1992. Haematological and biochemical studies on broiler chicks fed aflatoxin B1 and after its withdrawal. Indian Journal of Poultty Science, 27: 153-156.
Shinozuka, J., M. Suzuki, N. Noguchi, T. Sugimoto, K. Uetsuka, H. Nakayama, K. Doi, 1998. T2 toxin-inducedapoptosis in hematopoietic tissues of mice. Toxicologic Pathology 26, 674–681.
Sudakin, D. L., 2003. Trichothecenes in the environment: relevance to human health. Toxicology Letters, 143: 97-107.
Terao, K., Y. Ueno,1978. Morphological and functional damage to cells and tissues.In Toxicology, Biochemistry, and Pathology of Mycotoxins. Uraguchi, K. and Yamazaki,M. Eds.John Wiley and Sons, New York. p.189
Thrasher, J. D., 2012. Aflatoxicosis in animals. Aflatoxins and Health, www.alphaboostjuice. com/AFLATOXICOSIS_IN_ANIMALS.pdf;.
Tuzcu, M., E. Sur, I. Celik, Y. Oznurlu, M. K. Ciftci, 2010. Effects of aflatoxin on the proportions of peripheral blood leukocyte and Alpha-Naphtyl Acetate Esterase (ANAE) positive lymphocyte in the mouse. Kafkas University Vetinaty Fakultesi Dergisi, 16, 337-341.
Van Kessel, T. F. M., N. Hiang-Chek, 2004. Aflatoxin binders-how to get the best value for money. International Poultry Production, 12, 4, 33-35.
Wilson, D. M., G. A. Payne, 1994. Factors affecting Aspergillus Flavus group infection and aflatoxin contamination of crops. In: Eaton, D.L. Groopman,J.D. (Eds.). The toxicology of Aflatoxins. Academic Press inc, San Diego, pp123-145.
Wu, Q., A. Jezkova, Z. Yuan, L. Pavlikova, V. Dohnal, K. Kuca, 2009. Biological degradation of aflatoxins. Drug Metabolism Reviews, 41, 1, 1–7.
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