A COMPARATIVE STUDY ABOUT THE SENSITIVITY OF THE COMPLEMENT FIXATION TEST (СFT) IN DIAGNOSTIC OF THE DISEASE BRUCELLOSIS IN COWS HELD IN 4 0 С AND 37 0 С
Abstract
It was made comparative test about the sensitivity of the CFT method in in diagnostic of the disease brucellosis in cows. The reaction was placed ad 40 С and 370 С. The obtained results that both variants of the CFT were with equal sensitivity and specificity. This allows diagnostic laboratory to choose under what kind of conditions to work.
References
Костов, Г., 1995. Сравнително изпитване на диагностичната стойност на РСК на "топло" и "студено" със серуми от кочове с естествена инфекция Brucella ovis. Ветеринарна сбирка, 9-10; 15-21.
Масалски, Н., 2008. Заразни болести по овцете. ИК "Еньовче", С.
Решение на Комисията от 4 март 2004 година относно одобряване на тестовете за откриване на антитела против бруцелоза по говедата в рамките на Директива 64/432/ЕИО на Съвета (нотифицирано под номер С (2004) 654).
Al Dahouk, S., H. Tomaso, K. Nockler, H. Neubauer, D. Frangoulidis, 2003. Laboratory based diagnosis of brucellosis: A review of the literature. Part I: Techniques for direct detection and identification of Brucella spp. Clin Lab; 49:487-505.
Burgess G. W, M. J. Norris, 1982. Evaluation of the cold complement fixation test for diagnosis of ovine brucellosis. Australian Veterinary Journal, 1; 23-25.
Mantur B. G, A. S. Akki, S. S. Mangalgi, S. V. Patil, R. H. Gobbur, B. V. Peerapur, 2004. Childhood brucellosis - a microbiological, epidemiological and clinical study. J Trop Pediatr; 50 :1537.
Niilo L, W Donald, Mac Donald, F Gordon, and M. William Stone, 1986. Ovine Brucellosis in Alberta Can Vet J. 27(6), 245-249
Normative references
Bovine brucellosis, In: The OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees), 6th Edition 2008, Chapter 2.4.3, OIE, Paris, 624-659. http://www.oie.int/eng/normes/mma nual/2008/pdf/2.04.03_BOVINE_BRU CELL.pdf
Commission Decision of 10 December 2008 (2008/984/EC) amending Annex C to Council Directive 64/432/EEC and Decision 2004/226/EC as regards diagnostic tests for bovine brucellosis (notified under document number C(2008) 7642). Official Journal of the European Union, L 352/38-L 352/45.
ISO/IEC 17025, General requirements for the competence of testing and calibration laboratories.
OIE Quality Standard and Guidelines for Veterinary Laboratories, 2nd Edition, 2008, OIE, Paris.
Norme Francaise (French Standard) NF U 47-004 - Methodes d'analyse en sant? animale, Recherche d'anticorps contre la brucellose par la micro-m?thode de fixation du compl?ment, avril 2009, AFNOR, France.
Manual of diagnostic tests and vaccines for terrestrial animals, 2008. Office International des Epizooties, Paris, France.
Ramirez-Pfeiffer C., K. Nielsen, P. Smith, F. Marin-Ricalde, C. Rodriguez-Padilla, and R. Gomez-Flores, 2007. Application of the Fluorescence Polarization Assay for Detection of Caprine Antibodies to Brucella melitensis in Areas of High Prevalence and Widespread Vaccination. Clinical and Vaccine Immunology, March, Vol. 14, No. 3, 299-303.
Ridler A. L., D. M. West, K. J. Stafford, P. R. Wilson, M. G. Collett, 2002. Effects of vaginal Brucella ovis infection of red deer hinds on reproductive performance, and venereal transmission to stags. New Zealand Veterinary Journal, Vol. 50, N 4, 1, 126-131(6).
Wanke, M. M., M. V. Delpino, P. C. Baldi, 2002. Comparative performance of ELISA assay using cytosolic or outer membrane antigens of Brucella for the serodiagnosis of canine brucellosis. Vet. Microbiol, 88, 367-375.
Searson, J., 2000. Sensitiviti and spcifity of two microtiter complement Ficsation test for the diagnosis of Brucella ovis infection in rams. Aust.Vet. J.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2011 Bulgarian Journal of Animal Husbandry

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

