DNA - MICROSATELLITE ANALYSIS OF THE THOROUGHBRED HORSE POPULATION IN BULGARIA. DETERMINING THE NUMBER OF ALLELES AND ALLELE FREQUENCY IN STUDIED LOCI
Keywords:
Thoroughbred, microsatellites, allele number, allele frequency, genetic variationAbstract
The study includes 157 Thoroughbred horses enrolled either in the Bulgarian Thoroughbred Stud
Book or in the Non-Thoroughbred Register of the breed. We examined the observed number of alleles
and the allele frequency in 13 loci. The results were analyzed as for the whole population as well for
the most prominent lines within the population in our country. For the whole population we determined
total of 72 alleles, which number varied from 3 in loci HMS1 and HTG7 to 9 in locus ASB2. Most of
the loci were highly polymorphic which defines the high genetic diversity in the population. The number
of observed alleles varies considerably among the sire lines. In terms of that the sire lines of Blacklock
and Birdcatcher shows similar values for the number of observed alleles – 64. For the lines of Hurry On
and Tourbillon the number of observed alleles is respectively: 32 and 36. Analogical are the variations
of allele frequencies among the sire lines.
References
Barzev, G., E. Zhelyazkov, V. Barzeva, D. Hristova, Zh. Sabev, 2010. Genetic diversity in Bulgarian Thoroughbred using microsatellite DNA markers.- Agricultural science and technology 2 (3), 116-120.
Bower, M. A., M. G. Campana, M. Whitten, C. J. Edwards, H. Jones, E. Barrett, R. Cassidy, R. E. R. Nisbet, E. W. Hill, C. J. Howe, M. Binns, 2011. The cosmopolitan maternal heritage of the Thoroughbred racehorse breed shows a significant contribution from British and Irish native mares. Biol. Lett. 7, 316-320.
Bowling, A. T., W. Zimmermann, O. Ryder, C. Penado, S. Peto, L. Chemnick, N. Yasinetskaya, T. Zharkikh, 2003. Genetic variation in Przewalski’s horses, with special focus on the last wild caught mare, 231 Orlitza III.- Cytogenet Genome Res. 102(1-4), 226-234.
Choi, S. K., S. Y. Lee, G. J. Cho, 2012. Individual identification and parentage verification of Thoroughbred horses and the Korean native horses based on microsatellite loci in Korea.- J. Anim. Vet. Adv. 11 (15), 2647-2651.
Georgescu, S. E., M. Costache, 2012. Genetic characterization of Romanian local breeds using microsatellite markers.- Analysis of Genetic Variation in Animals, Prof. Mahmut Caliskan (Ed.), ISBN: 978-953-51-0093-5, InTech, DOI:10.5772/32960.
Georgieva, S., E. Todorovska, D. Hristova, I. Dimitrova, N. Stancheva, Ts. Yablanski, 2013. Genetic diversity and distance between two Bulgarian local sheep breeds assessed by microsatellite markers. – Agricultural Science and Technology 5 (4), 367 – 370.
Jakabová, D., J. Trandžík, J. Chrastina, Ľ. Hudecová, E. Zetochová, J. Bulla, A. Bugarský, F. Jakab, P. Kozlík, 2002. Effectiveness of six highly polymorphic microsatellite markers in resolving paternity cases in Toroughbred horses in Slovakia.-Czech J. Anim. Sci., 47 (12), 497–501.
Lee, J. E., J. H. Shin, Y. M. Yun, K. K.Lee, H. Lee, O. K. Kweon, Y. S. Yun, J. G. Suh, N. S. Shin, J. K. Seong (2010) - Genetic polymorphism of Jeju Horses by microsatellite DNA markers in Korea.- Lab. Anim. Res. 26 (2), 219-221.
Lee, S. Y., G. J. Cho, 2006. Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing.- J. Vet. Sci. 7(1), 63–67.
Leroy, G., L.Callède, E. Verrier, J. C. Mériaux, A. Ricard, C.Danchin-Burge, X. Rognon, 2009. Genetic diversity of a large set of horse breeds raised in France assessed by microsatellite polymorphism.- Genetics Selection Evolution,41 (5), doi:10.1186/1297-9686-41-5.
Silva, A. C. M., S. R. Paiva, M. S. M. Albuquerque, A. A. Egito, S. A. Santos, F. C. Lima, S. T. Castro, A. S. Mariante, P. S. Correa, C. M. McManus, 2012. Genetic variability in local Brazilian horse lines using microsatellite markers.- Genet. Mol. Res. 11 (2), 881-890.
Sunnucks, P., 2001. Efficient genetic markers for population biology.- Tree 15, 199–203.
Zabek, T., M. Duniec, M. Bugno, 2003. Genetic relationships between Silesian, Thoroughbred and Oldenburg horses based on DNA microsatellite polymorphism.- Annals of Animal Science 3 (2), 213-224.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2014 Bulgarian Journal of Animal Husbandry
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
