IFLUENCED OF AFLATOXIN B1 ON LIVER AND PANCREATIC FUNCTION IN MULARD DUCKS WITH EXPERIMENTAL AFLATOXICOSIS B1
Keywords:
aflatoxicosis В1, toxicity, duckling, liver and pancreatic damage, Mycotox NGAbstract
Ducklings are among the most sensitive avian species to the toxic effects of aflatoxin В1 (AFB1). In this experiment, the toxic effects of AFB1 on liver and pancreatic morphology, blood plasma aspartate amino-transferase (AST), alanine amino-transferase (ALT), alkaline phosphatase (AP), lactate dehydrogenase (LDH), γ-glutamyltransferase (γ-GT), total protein (TP), albumin, blood glucose, cholesterol, triglyceride, alpha amylase, lipase and insulin were established in mulard ducks. The experiment was carried out with four groups of 20 10-day-old ducklings each. Each group included three subgroups with 10 birds. The groups were as followed: group І – control (which received standard feed according to the species and age), group ІІ – experimental, which received compound feed with 0.5 mg/kg AFB1, group ІІI– receiving compound feed supplemented with 0.8 mg/kg AFB1 and group IV – compound feed supplemented with 0.5 mg/kg AFB1
and 2g/kg Mycotox NG. The experiment lasted for 42 days. Blood biochemical data on the 21st day of the experiment showed increased activities of AST, ALT, AP, LDH, γ-GT and decrease activities of alpha amylase and lipase in groups receiving only AFB1 . Total protein, albumin, cholesterol, triglycerides and blood glucose were lower than respective control values. The observed changes tended to become more pronounced by the 42nd day of the trial. Insulin concentrations in experimental groups of mulards were insignificantly higher during both sampling intervals compared to
untreated birds (control group) (Р>0.05). Histopathologically, the liver exhibited a various extent of dystrophy was detected depending on the dose of ingested toxin (hepatocytes exhibited granular degradation and cytoplasm vacuolation, karyorrhexis, karyopyknosi, fatty dystrophy, erivascular mononuclear
infiltrations, areactive necrotic, epithelium of biliary ductules was hyperplastic). Histopathologically, the
pancreas exhibited intralobular swelling, disorganisation and disintegration of glandular acini, various extents of dystrophic changes, mononuclear infiltrates as well as necrobiotic changes depending on the dose of ingested toxin. The supplementation of feed of group IV with Mycotox NG improved deviations in blood biochemical parameters and substantially reduced the severity and prevalence of histological lesions.
References
Наредба №44/20.04.2006 г. за ветеринарномедицинските изисквания към животновъдните обекти , ДВ 41 Приложение № 9/ІІ, стр.61-62.
Abd El-Haleem, M. R., A. M. Dalia, 2011. The effects of experimental aflatoxicosis on the pancreas of adult male albino rats and the role of ginger supplementation: a histological and biochemical study. Egyptian Journal of Histology, 34, 3, 423-435.
Abdel-Wahhab, M. A., S. A. Nada, F. A. Khalil, 2002. Physiological and toxicological responses in rats fed aflatoxincontaminated diet with or without sorbent materials. Animal Feed Science and Technology, 97, 209–219.
Afzali, N., G. Devegowda, 1999. Ability of modified mannanoligosaccharide to counteract aflatoxicosis in broiler breeder hens. Poultry Science, 78, (Suppl. 1), 228. (Abstr.).
Agag, B. I., 2004. Mycotoxins in foods and feeds : Aflatoxins. Association of Universal Bullettin of Environmental Research, 7, 1, 173-191.
Al-Jubory, K. M. T., 2000. The effect of kaolin on pancreatic digestive enzymes during aflatoxicosis in broiler chicks. Journal Iraqi Journal of Veterinary Sciences, 13, 1,: 15-20.
Aravind, K. L., V. S. Patil, G. Devegowda, B. Umakantha, S. P. Ganpule, 2003. Efficacy of esterified glucomannan to counteract mycotoxicosis with naturally contaminated feed on performance and serum biochemical and hematological parameters in broilers. Poultry Science, 82, 571–576.
Awad, M. E., M. S. Abdel-Rahman, S. A. Hassan, 1998. Acrylamide toxicity in isolated at hepatocytes. Toxicology In Vitro 12, 699–704.
Bailey, R. H., L. F. Kubena, R. B. Harvey, S. A. Buckiey, G. E. Rottinghaus, 1998. Efficacy of various inorganic sorbents to reduce the toxicity of aflatoxin and T-2 toxin in broiler chickens. Poultry Science, 77, 11, 1623–1630.
Balachandran, C., R. Ramarkrishnan, 1988. Influence of dietary aflatoxin on certain serum enzyme levels in broiler chickens. Mycopathologia, 101, 2, 65-7.
Bennett, J. W., M. Klich, 2003. Mycotoxins. Clinical Microbiology Reviews, 16, 3, 497-516.
Bintvihok, A., S. Kositcharoenkul, 2006. Effect of dietary calcium propionate on performance, hepatic enzyme activities and aflatoxin residues in broilers fed a diet containing low levels of aflatoxin B1. Toxicon, 47, 41–46.
Calvo, A. M., J. Bok, W. Brooks, N. P. Keller, 2004. veA Is Required for Toxin and Sclerotial Production in Aspergillus parasiticus. Applied and Environmental Microbialogy, 70, 4733-4739.
Cambell, T. W., E. H. Coles, 1989. Patología Clinica de Aves. In: Diagnóstico y Patología Veterinaria,4th edn, pp. 285-308. (México, Editorial Interamericana).
Cardinet, III. G. H., 1989. Skeletal muscle function. In: KANEKO, J.J. (Ed.) Clinical Biochemistry of Domestic Animals, pp. 462-495. (San Diego, California, Academic Press, Inc).
Celik, K., M. Denli, M. Erturk, O. Ozturkcan, F. Doran, 2001. Evaluation of dry yeast Saccharomyces Cerevisiae in the feed to reduce aflatoxin B1 (AFB 1) residues and toxicity to Japonica Quails (Coturnix coturnix Japonica). Journal of Applied Animal Research, 20, 245-250.
Chang, C. F., P. Hamilton, 1982. Experimental aflatoxicosis in young Japanese quail. Poultry Science, 61, 5, 869–874.
Council for Agricultural Science and Technology, 1989. Pages 1–91 in: Mycotoxins: Economic and Health Risks. K. A. Nisi, ed. Task Force Report No. 116. Council for Agricultural Science and Technology, Ames, IA.
Cullen, J. M., P. M. Newberne, 1994. Acute hepatotoxicity of aflatoxins.In: Eaton, D.L., Groopman, J. D. (Eds.), Toxicology of Aflatoxins. Academic Press, San Diego, pp. 3–26.
D’Mello, J. P. F., 2003. Mycotoxins in cereal grains, nuts and other plant products. In: Food Safety Contaminants and Toxins. D’Mello, J P. F (Ed).Cromwell Press, Trowbridge, UK; 2003. 65-90.
Daković, A., M. Tomasevic-Canovic, V. Dondur, G. E. Rottinghaus, V. Medakovic, S. Zaric, 2005. Adsorption of mycotoxins by organozeolites. Colloids Surf. B Biointerfaces 46, 20–25.
Dalvi, R. R., 1986. An overview of aflatoxicosis of poultry: its characteristics, prevention and reduction. Vetewrinary Research Communications, 10, 429–443.
Denli, M., F. Okan, F. Doran, T. C. Inal, 2005. Effect of dietary conjugated linoleic acid (CLA) on carcass quality, serum lipid variables and histopathological changes of broiler chickens infected with aflatoxin B1. African Journal of Animal Science, 35, 109–116.
Denli, M., J. C. Blandon, M. E. Guynot, S. Salado, J. F. Perez, 2009. Effects of dietary AflaDetox on performance, serum biochemistry, histopathological changes, and aflatoxin residues in broilers exposed to aflatoxin B1. Poultry Science, 88, 1444-1451.
Dewegowda, G., T. N. K. Murthy, 2005. Mycotoxins: Effects on poultry,performance and health. In: The Mycotoxin Blue Book, Diaz D.E. (ed.), Nottingham University Press, Nottingham, 25-56.
Diaz, D. E., 2005. The Mycotoxin Blue Book, 1st ed.; Nottingham University Press: Nottingham, UK, pp. 25–56.
Diener, U. L., R. J. Cole, T. H. Sanders, G. A. Payne, L. S. Lee, M. A.. Klich, 1987. Epidemiology of Aflatoxin, in formation by Aspergillus flavus. Annual Review of Phytopathology 25, 240-270.
Eaton, D. L.,. D. Groopman, 1994. The toxicology of aflatoxins: human health, veterinary, and agricultural significance. Academic Press, San Diego, CA, 1994. Ehrlich, K.C. (2009). Predicted roles of uncharacterized clustered genes in aflatoxin biosynthesis, Toxins, 1, 37-58.
EEC, 1991. EEC Council Directive 91/126/EEC. Amending the annexes to Council Directive 74/63/EEC on undesirable substances and products in animal nutrition. Official Journalof the European, Community,No., L 60.
El-Agamy, D. S., 2010. Comparative effects of curcumin and resveratrol on aflatoxin B (1)-induced liver injury in rats. Archivies of Toxicology, 84, 389–396.
El-Nekeety, A. A., S. R. Mohamed, A. S. Hathout, N. S. Hassan, S. E. Aly & M. A. AbdelWahhab, 2011. Antioxidant properties of Thymus vulgaris oil against aflatoxin-induced oxidative stress in male rats.Toxicon, 57, 984-991.
European Commission, 2010. Commission Regulation (EU) No. 165/2010 of 26 February 2010 amending Regulation (EC) No. 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards aflatoxins. Official Journal of the European Union, L50, 8–12.
Fatemi, F., A. Allameh, A. Dadkhah, M., Forouzandeh, S. Kazemnejad, R. Sharifi, 2006. Changes in hepatic cytosolic glutathione S-transferase activity and expression of its class-P during prenatal and postnatal period in rats treated with aflatoxin B1. Archivies of Toxicology, 80, 572-579.
Gaskill, C. L., L. M. Miller, J. S. Mattoon, W. E. Hoffmann, S. A. Burton, H. C. J. Gelens, S. L. Ihle, J. B. Miller, D. H. Shaw, A. E. Cribb, 2005. Liver histopathology and liver serum alanine aminotransferase and akaline phosphatase activities in epileptic dogs receiving phenobarbital. Veterinary Pathology, 42, 147–160.
Hussein, H. S., J. M. Brasel, 2001. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology, 167, 101–134.
Ibrahim, I. K., A. M. Shareef, K. M. T. Al-Joubory, 2000. Ameliorative effects of sodium bentonite on phagocytosis and Newcastle disease antibody formation in broiler chickens during aflatoxicosis. Research in Veterinary Science, 69, 2, 119-122
Jakhar, K. K., J. R. Sadana, 2004. Sequential pathology of experimental aflatoxicosis in quail and the effect of selenium supplementation in modifying the disease process. Mycopathologia, 157:99–109.
Jindal, I. V., S. K. Mahipal, 1994. Toxicity of Aflatoxin B1 in broiler chicks and its reduction by activated charcoal. Research in Veterinary Science, 56, 37.
Jouany, J. P., 2007. Methods for preventing, decontaminating and minimizing the toxicity of mycotoxins in feeds. Anim. Feed Sci. Technol. 137, 342–362.
Kececi, T., H. Oguz, V. Kurttoglu, V. O. Demet, 1998. Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. British Poultry Science, 39, 452-458.
Krishnamoorthy, P., S. Vairamuthu, C. Balachandran, B. Muralimanohar, 2007. Pathology of chlorpyriphos and T-2 toxin on broiler chicken. Vetеисхьиянс arhiv, 77, 1, 47-57.
Kubena, L. F., R. B. Harvey, R. H. Baile, S. A. Buckley, G. E. Rottinghaus, 1998. Effects of hydrateted sodium calcium aluminosilicate [TBind] onmycotoxicosis in young broiler chickens. Poultry Science, 77, 1502–1509.
Kubena, L. F., R. B. Harvey, W. E. Huff, M. H. Elissalde, A. G. Yersin, T. D. Phillips and G. E. Rottinghaus, 1993a. Efficacy of a hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and diacetoxyscirpenol. Poultry Science, 72, 1, 51–59.
Kubena, L. F., R. B. Harvy, T. D. Phillips, B. A. Clements, 1993b. Effects of a hydrated sodium calcium aluminosilicate on aflatoxicosis in broiler chicks. Poultry Science, 72, 4, 651-657.
Ledoux, D. R., G. E. Rottinghaus, A. J. Bermudez, M. Alonso-Debolt, 1998. Efficacy Of a Hydrated Sodium Calcium Aluminosilicate To Ameliorate The Toxic Effects Of Aflatoxin In Broiler Chicks. Poultry Science, 77, 204–210.
Leeson, S., G. J. Diaz, J. D.Summers, 1995. “Poultry Metabolic Disorders And Mycotixns.” Pp. 249-298, University Books, Guelph, Ontario, Canada.
Matur, E., E. Ergul, I. Akyazi,, E. Eraslan, Z, T. Ciraklit, 2010. The effects Saccharomyces cerevisiae extract on th weight of some organs, liver and pancreatic digestive enzyme activity in breeder hens fed diets contaminated with aflatoxins. Poultry Science, 89, 2213-2220.
Mckenzie, K. S., L. F. Kubena, A. J. Denvir, T. D. Rogers, G. D. Hitchens, R. H. Bailey, R. B. Harvey, S. A. Buckley, T. D. Phillips, 1998. Aflatoxicosis In Turkey Poults Is Prevented By Treatment Of Naturally Contaminated Corn With Ozone Generated By Electrolysis. Poultry Science, 77, 8, 1094-102.
Miazzo R., C. A. Rosa, E. C. De Queiroz Carvalho, C. Magnoli, S. M. Chiacchiera, G. Palacio, M. Saenz, A. Kikot, E. Basaldella, A. Dalcero, 2000. Efficacy of synthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks. Poultry Science, 79, 1–6.
Mohamed, M. A., M. H. Mohamed, 2009. Haemato-biochemical and pathological studies on aflatoxicosis and treatment of broiler chicks in Egypt. Veterinaria Italiana, 45, 2, 323-37.
Mohammed, A. M., N. S. Metwally, 2009. Antiaflatoxicogenic activities of some aqeous plant extracts against AFB1 induced Renal and Cardiac damage. Journal of Pharmacology and Toxicology, 4, 1, 1-16.
Monbaliu S., C. Van Poucke, C. Detavernier, F. Dumoulin, M. Van De Velde, E. Schoeters, S. Van Dyck, O. Averkieva, C. Van Peteghem, S. De Saeger, 2010. Occurrence of mycotoxins in feed as analyzed by a multi-mycotoxin LC-MS/MS method. Journal of Agricultural and Food Chemistry, 58, 66–71.
Nomura H., M. Ogiso, M. Yamashita, H. Takaku, A. Kimura, M. Chikasou, Y. Nakamura, S. Fujii, M. Watai, H.Yamada, 2011. Uptake by dietary exposure and elimination of aflatoxins in muscle and liver of rainbow trout (Oncorhynchus mykiss). Journal of Agricultural and Food Chemistry, 59, 5150–5158.
Ominski, K. H., R. R. Marquardi, R. N. Sinha, D. Abramson, 1994. Ecological aspects of growth and mycotoxin production by storage fungi. In: Miller, J.D and Trenholm, HL. Mycotoxins in grains: Compounds other than aflatoxins. Eagan Press, St. Paul Minnesota, USA.1994; 287-314.
Ortatatli, M., H. Oguz, F. Hatipoglu, M. Karaman, 2005. Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Research in Veterinary Science, 78, 61–68.
Osborne, D. J., P. B. Hamilton, 1981. Decreased pancreatic digestive enzymes during aflatoxicosis. Poultry Science, 60, 8, 1818–1821.
Pang, V. F., J. H. Adams, V. R. Beasley, W. R. Buck, W. M. Haschek, 1986. Myocardial and Pancreatic Lesions Induced by T-2 Toxin, a Trichothecene Mycotoxin, in Swine. Veterinary Pathology, 23, 3, 310-319.
Parlat, S. S., M. Ozcan, H. Oğuz, 2001. Biological suppression of aflatoxicosis in Japanese quail (Coturnix coturnix japonica) by dietary addition of yeast (Saccharomyces cerevisiae). Researcr in Veterinary Science, 71, 207–211.
Pier, A. C., 1981. Mycotoxins and animal health. Advances in Veterinary Sciences and Comparative Medicine, 42, 185–243.
Qazi, J. I., Z. Fayyaz, 2006. Aflatoxin contaminated foods and health risk perspective for Pakistani population (Review). Mycopathologia, 4, 27-34.
Rahman, M. F., M. K. Siddiqui, K. Jamil, 2000. Acid and alkaline phosphatase activities in a novel phosphorothionate (RPR-11) treated male and female rats. Evidence of dose and time-dependent response. Drug and Chemical Toxicology, 23, 497–509.
Raju, M., G. Devegowda, 2000. Influence of esterifiedglucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin). British Poultry Science, 41, 640–650.
Ramos, A. .J., E. Hernandez, 1997. Prevention of aflatoxicosis in farm animals by means of hydrated sodium calcium aluminosilicate addition to feedstuff: A review. Animal Feed Science and Technology 65, 197-206.
Recknagel, R. O., E. A. Glende, J. A. Dolak, R. L. Waller, 1989. Mechanisms of carbon tetrachloride toxicity. Pharmacology and Therapeutics, 43, 139–154.
Riley R. T., J. Pestka, 2005. Mycotoxins: Metabolism, mechanism, and biochemical arkers. In: The Mycotoxin Blue Book, Diaz D.E. (ed.), Nottingham University Press, Nottingham, 2005, pp.: 279-294.
Rosa, C. A. R., R. Miazzo, C. Magnoli, M. Salvano, S. M./ Chiacchiera, S. Ferrero, M. Saenz, E. C. Q. Carvalho, A. Dalcero, 2001. Evaluation of the efficacy of bentonite from the south of Argentina to ameliorate the toxic effects of aflatoxin in broilers. Poultry Science, 80, 2, 139-144.
Safameher, A., 2008. Effects of clinoptilolite on performance, biochemical parameters and hepatic lesions in broiler chickens during aflatoxicosis. Journal of the American Veterinary Association, 7, 381-388.
Saif, Y. M., H. J. Barnes, J. R. Glissons, L. R. McDougald, D. E. SwayneEd, 2003. Disseases of poutry, 11th. Editional Board for the American Association of Avian Pathologists. Masby-Wolfe, Iowa, State University, Press, Ames, Iowa, 320-326.
Sakhare, P. S., S. D. Harne, D. R. Kalorey, S. R. Warke, A. G. Bhandarkar, N. V. Kurkure, 2007. Effect of Toxiroak® polyherbal feed supplement during induced aflatoxicosis, ochratoxicosis and combined mycotoxicoses in broilers. Veterinarski Arhiv, 77, 129–146.
Sawhney, D. S., P. V. Vadehra, R. C. Baker, 1973. Aflatoxicosis in the laying Japanese quail (Coturnix coturnix japonica). Poulttry Sciemce, 52, 465–473
Schneideled, S. E., 1993. Effects of various types of aluminosilicates and aflatoxin B1 on aflatoxin toxicity, chick performance, and mineral status. Poultry Science, 72, 282-288.
Sharma, V., C. Sharma, R. Paliwai, Prachetal, S. Sharma, 2011. Ameliorative Effects of Curcuma Longa and Curcumin on Aflatoxin B1 Induced Serological and Biochemical Changes In Kidney of Male Mice. Asian Journal of Biochemical and Pharmaceutical Research, 1, 2, 338-351.
Shi, Y. H., Z. R. Xu, J. L. Feng, C. Z. Wang, 2006. Efficacy of modified montmorillonite nanocomposite to reduce the toxicity of aflatoxin in broiler chicks. Animimal Feed Science and Technology, 129, 138–148.
Shotwell, O. L., 1991. In: 1. E. Smith and R. Henderson (eds.). Mycotoxins and Animal Foods. CRC Press: Boca Raton, FL, 325-340.
Shoyinka, S. V. O., E. O. Onyekweodiri, 1987. Clinicopathologyof interaction between aflatoxin and aspergillosis inchickens. Bulletin of Animal Health Production and Africa, 35, 47-51.
Simsek, N, L. Ergun, R. Alabay, D. Essiz, 2007. The effects of experimental aflatoxicosis on the exocrine pancreas in quails (Coturnix coturnix japonica). Archives of Toxicology, 81, 8, 583–588.
Valdivia, A. G., A. Martınez, F. J. Damian, T. Quezada, R. Ortız, C. Martınez, J. Llamas, M. L. Rodrıguez, L, Yamamoto, F. Jaramillo, M. G. Loarca-Pina, G. L. Reyeя, 2001. Efficacy of N-Acetylcysteine to Reduce the Effects of Aflatoxin B1 Intoxication in Broiler Chickens. Poultry Science, 80, 6, 727–734.
Wangikar, P. B., P. Dwivedi, N. Sinha, A. K. Sharma, A. G. Telang, 2005. Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicology, 215, 37–47.
Wu, F., G. P.Munkvold, 2008. Mycotoxins in ethanol coproducts:modeling economic impacts on the livestock industry and management strategies. Journal of Agricultural and Food Chemistry, 56, 3900–3911.
Yener, Z., I. Celik, F. Ilhan, R. Bal, 2009. Effects of Urtica dioica L. seed on lipid peroxidation, antioxidants and liver pathology in aflatoxin induced tissue injury in rats. Food Chemical Toxicology, 47, 2, 418–424.
Yildirim, E., I. Yalchinkaya, M. Kanbur, M. Çnar, E. Oruc, 2011. Effects of yeast lucomannan on performance, some biochemical parameters and pathological changes in experimental aflatoxicosis in broiler chickens. Revue de Médicine Véterinaure, 162, 8-9, 413-420.
Yoshizawa, T., 1991. In: J. E. Smith and R. Henderson (eds.). Mycotoxins and Animal Foods. CRC Press: Boca Raton, FL, 301-324.
Zhang, Y., J. Caupert, 2012. Survey of mycotoxins in U.S. distiller’s dried grains with solubles from 2009 to 2011. Journal of Agricultural and Food Chemistry, 60, 539–543.
Zhao, J., R. B. Shirley, J. D. Dibner, F. Uraizee, M. Officer , M. Kitchell, M. VazquezAnon, C. D. Knight, 2010. Comparison of hydrated sodium calcium aluminosilicate and yeast cell wall on counteracting aflatoxicosis in broiler chicks. Poultry Science, 89, 2147–2156.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2015 Bulgarian Journal of Animal Husbandry
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
