MODIFIED METHOD FOR LONG-TERM STORAGE OF LIVE TAENIID EGGS IN LIQUID CULTURE

Authors

  • K. Kanchev University of Forestry, Faculty of Veterinary Medicine – Sofia Author

Keywords:

modified method, Taenia hydatigena, taeniid eggs, liquid culture, infectivity

Abstract

The aim of the experimental work was the development and studying of a new modification of the established in the parasitological practice method for preparation and storage of taeniid eggs in liquid culture.
For model in experiments were used the eggs of the parasite Taenia hydatigena. Results for the morphology and viability of the eggs of the tapeworm for 2 years period have been received. Infectivity of eggs from culture has been studied on typical intermediate hosts sheep and pigs.
The developed modified method for the preparation and long term storage of live taeniid eggs in liquid culture can be used in the parasitological practice for experimental and diagnostic purposes.

References

Акабаев, И. Ш., А. А. Водянов, Н. Е. Косминков, 2002. Паразитология и инвазионыe болезни животных, II издание, “Колосс”, Москва.

Илиев, Е., Ю. Бегов, К. Кънчев, Й. Каменов, Д. Илиева, 2009. Тежък случай на тенуиколна цистицеркоза при агне. Сборник с научни трудове от “Международната научно-практическа конференция на НДНИВМИ”, София, 209-215.

Камбуров, П., Ив. Василев, Д. Георгиева, Й. Каменов, В. Койнарски, 1994. Ветеринарномедицинска паразитология. Агропрес, София.

Каменов, Й., К. Кънчев, 2010. Ларвалната ехинококоза – стопански, здравен и социален проблем за България. Сборник с научни трудове от международната научна конференция“Традиции и съвременност във ветеринарната медицина”, София, 397 – 404.

Каменов, Й., К. Кънчев, В. Радев, 2009. Проучвания върху хелминтната инвазия на каниди от Северозападна България. Сборник с научни трудове от международната научна конференция “Традиции и съвременност във ветеринарната медицина”, София, 298-303.

Каменов, Й., В. Радев, 2002. Ръководство за упражнения по ветеринарна паразитология. Издателска къща „Пандора”, Хасково.

Кънчев, К, 2013. Проучвания върху тенуиколната цистицеркоза в България. Дисертация за придобиване на О.Н.С. „Доктор”, Лесотехнически университет, София.

Кънчев, К., Й. Каменов, К. Саламитова, П. Радева, 2014. Хелминтен статус на домашни кучета с гастро-ентерити от района на град София. Сборник научни трудове от международната конференция „20 години Факултет по ветеринарна медицина при ЛТУ”, Юндола (под печат).

Bamorovat, M., M. H. Radfar, A. Derakhshanfar, M. Molazadeh, M. B. Zarandi, 2014. A comparative evaluation of hematological, biochemical and pathological changes among infected sheep with Cysticercus tenuicollis and non-infected control group. J. Parasit. Dis., Dec. 38(4):399-403.

Brandt, J., M. Sewell, 1981. In vitro hatching and activation of Taenia taeniaeformis oncospheres. Vet. Research Communications, 5, 193-199.

Brglez, J., 1997. A fatal case of Cysticercus tenuicollis infestation in sheep. Veterinarske Novice, Vol: 23, Issue: 4, Pages: 140-141.

Bowman, D., 2014. Georgis’ Parasitology for Veterinarians, Tenth ed.”, Saunders, Elsevier, USA.

Chartier, C., G. Perrin, F. Marty, 1995. Cysticercosis (C. tenuicollis) with hepato-peritoneal sequelae in a female goat. Point Vйtйrinaire, Vol: 26, Issue: 166, Pages: 1171-1174.

Kamya, M., H. Sato, 1990. Complete life cycle of the canid tapeworm Echinococcus multilocularis in laboratory rodents. FASEB Journal, Vol.4, p. 3334-3339.

Manfredi, M. T., R. Ghirardelli, S. Zanzani, 2006. Cysticercus tenuicollis infection in a goat farm. Parassitologia (Roma), Vol: 48, Issue: 3, Pages: 433-436 (in italian).

Martнnez-Moreno, F. J., S. Hernбndez, E. LуpezCobos, C. Becerra, I. Acosta, A. Martнnez-Moreno, 2007. Estimation of canine intestinal parasites in Cуrdoba (Spain) and their risk to public health. Veterinary Parasitology, Vol: 143, Issue: 1, Pages: 7-13.

Mathis, A., P. Deplazes, 2006. Copro-DNA tests for diagnosis of animal taenniid cestodes. Parasitology International, 55:S87-90.

Miyaji, S., Y. Oku, M. Kamiya, M. Okamoto, M. Ohbayashi, A. Uchida , R. L. Rausch, 1990. Growth of a Japanese isolate of Taenia crassiceps in intermediate and definitive hosts, Parasitol. Res., 76:351 354.

European Food Safety Authority (EFSA) Community Summary Report, 2010. Trends and Sources of Zoonoses and Zoonotic Agents and Food-borne Outbreaks in the European Union in 2008, 8(1):234-237.

Pathak, K. M. L., S. N. S. Gaur, S. N. Sharma, 1982. The pathology of Cysticercus tenuicollis infection in goats. Vet. Parasitol., 11: 131-139.

Rainova, I., Ir. Marinova, R. Harizanov, D. Jordanova, I. Kaftandjiev, Iv. Bikov, N. Tsvetkova, 2014. Parasitic diseases in Bulgaria in 2012. Probl. Inf. Parasit. Dis., Vol. 42,1.

Samojlowicz, M., 2006. Tapeworm invasions in carnivores in the Lublin region, and problems with diagnostics. Życie Weterynaryjne, Vol: 81, Issue: 6, Pages: 404-407.

Smith, D., 1990. In vitro cultivation of parasitic helminths. CRC Press Inc., US.

Soulsby, E. J. L., 1982. Helminths, Arthropods and Protozoa of Domesticated Animals. 7th Ed., Baillere Tindall, London.

Taylor, M. A., R. L. Coop, R. L. Wall, 2007. Veterinary Parasitology, Third edition, Blackwell Publishing, Oxford, UK.

Thiel, W., M. Takla, 1983. Fatal outcome of mass infestation with Cysticercus tenuicollis in swine. Tierarztliche Praxis, Vol: 11, Issue: 1, Pages: 47-52.

Upton, J., 2005. Animal Parasitology, Biology 625, Laboratory Manual, Kansas State University, USA.

Wallis, P. M., H. M. Wallis, 1986. Excystation and culturing of human and animal Giardia spp. by using gerbils and TYI-S-33 medium. App. and Env. Microbiology, Vol. 50, N3, p. 647-651.

Xhaxhiu, D., Il. Kusi, Dh. Rapti, El. Kondi, R. Postoli, L. Rinaldi, Zl. M. Dimitrova , M. Visser , M. Knaus, St. Rehbein, 2011. Principal intestinal parasites of dogs in Tirana, Albania. Parasitol. Res., 108:341–353.

Yildirim, А., A. Эca, L. Beyaz, A. Atasaver, 2006. Acute hepatitis cysticercosa and Pneumonitis cysticercosa in a lamb: Case report. Tьrkiye Parazitoloji Dergisi (Acta Parasitologica Turcica), 30 (2): 108-111(in turkish).

Published

23.12.2015

How to Cite

Kanchev, K. (2015). MODIFIED METHOD FOR LONG-TERM STORAGE OF LIVE TAENIID EGGS IN LIQUID CULTURE. Bulgarian Journal of Animal Husbandry, 52(6), 35-39. https://agriacad.eu/ojs/index.php/bjah/article/view/373