Effect of cumulus cells on vitrification of matured bovine oocytes
Keywords:
bovine, cumulus cells, in vitro, oocytes, vitrificationAbstract
In order to implement the best vitrification protocol one must minimize the damage that the oocytes suffer during the process and achieve a higher rate of survival and development. To realize this goal, it is necessary to determine the effect of cumulus cells during vitrification of matured bovine oocytes.
The survival rate of fresh oocytes was very close to 100%, with no significant differences between groups. However, the survival rates of vitrified denuded oocytes (vDO) in groups (91.0%) and vitrified denuded oocytes with cumulus cells (vDO + cc) (93%) were higher than vitrified cumulus-oocyte
complexes (COC) (79.6%), P < 0.05. Vitrified COCs had significantly lower survival rates (79.6%) compared to their fresh counterparts 97.3% respectively, (P < 0.05). Our results indicate that it is advisable to remove at least some of the cumulus cells before vitrification of matured bovine oocytes. Denuded oocytes undergo vitrification at a higher rate than cumulusoocyte complexes, although their fertilization and subsequent embryo development are compromised due to the lack of cumulus cells. Addition of intact COCs can restore the situation, providing denuded oocytes with similar rates of fertilization and embryo development as vitrified COCs.
References
Chinen, S., Yamanaka, T., Hirabayashi, M., & Hochi, S. (2020). Rescue of vitrified-warmed bovine mature oocytes by short-term recovery culture with resveratrol. Cryobiology, 97, 185-190.
Dujíčková, L., Makarevich, A. V., Olexiková, L., Kubovičová, E., & Strejček, F. (2021). Methodological approaches for vitrification of bovine oocytes. Zygote, 29(1), 1-11.
Gasparrini, B., Attanasio, L., De Rosa, A., Monaco, E., Di Palo, R., & Campanile, G. (2007). Cryopreservation of in vitro matured buffalo (Bubalus bubalis) oocytes by minimum volumes vitrification methods. Animal Reproduction Science, 98(3-4), 335-342.
Kuwayama, M., Vajta, G., Kato, O., & Leibo, S. P. (2005). Highly efficient vitrification method for cryopreservation of human oocytes. Reproductive biomedicine online, 11(3), 300-308.
Massip, A., Van Der Zwalmen, P., & Ectors, F. (1987). Recent progress in cryopreservation of cattle embryos. Theriogenology, 27(1), 69-79.
Paul, A. K., Liang, Y., Srirattana, K., Nagai, T., & Parnpai, R. (2018). Vitrification of bovine matured oocytes and blastocysts in a paper container. Animal Science Journal, 89(2), 307-315.
Rall, W. F., & Fahy, G. M. (1985). Ice-free cryopreservation of mouse embryos at− 196 C by vitrification. Nature, 313(6003), 573-575.
Rizos, D., Ward, F., Duffy, P. A. T., Boland, M. P., & Lonergan, P. (2002). Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Molecular reproduction and development, 61(2), 234-248.
Seidel Jr, G. E. (2006). Modifying oocytes and embryos to improve their cryopreservation. Theriogenology, 65(1), 228-235.
Sinha, R., Kharche, S. D., Sinha, N. K., Goel, A. K., Jindal, S. K., & Sonia, S. (2014). Effect of vitrification and slow freezing on in-vitro matured prepubertal goat oocytes. Indian Journal of Animal Sciences, 84(3), 289-292.
Somfai, T., & Hirao, Y. (2021). Vitrification of immature bovine oocytes in protein-free media: The impact of the cryoprotectant treatment protocol, base medium, and ovary storage. Theriogenology, 172, 47-54.
Tashima, K., Kubo, Y., Hirabayashi, M., & Hochi, S. (2017). Downsizing cumulus cell layers to improve cryotolerance of germinal vesicle-stage bovine oocytes. Theriogenology, 95, 1-7.
Vajta, G., Holm, P., Kuwayama, M., Booth, P. J., Jacobsen, H., Greve, T., & Callesen, H. (1998). Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Molecular Reproduction and Development: Incorporating Gamete Research, 51(1), 53-58.
Vieira, A. D., Mezzalira, A., Barbieri, D. P., Lehmkuhl, R. C., Rubin, M. I. B., & Vajta, G. (2002). Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology, 45(1), 91-94.
Whittingham, D. G., Leibo, S. P., & Mazur, P. (1972). Survival of mouse embryos frozen to-196 and-269 C. Science, 178(4059), 411-414.
Zhang, J., Nedambale, T. L., Yang, M., & Li, J. (2009). Improved development of ovine matured oocyte following solid surface vitrification (SSV): effect of cumulus cells and cytoskeleton stabilizer. Animal reproduction science, 110(1-2), 46-55.
Zhang, F., Zhang, Z. Y., Cai, M. D., Li, X. X., Li, Y. H., Lei, Y., & Yu, X. L. (2020). Effect of vitrification temperature and cryoprotectant concentrations on the mRNA transcriptome of bovine mature oocytes after vitrifying at immature stage. Theriogenology, 148, 225-235.
Zhou, X. L., Al Naib, A., Sun, D. W., & Lonergan, P. (2010). Bovine oocyte vitrification using the Cryotop method: effect of cumulus cells and vitrification protocol on survival and subsequent development. Cryobiology, 61(1), 66-72.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2023 Bulgarian Journal of Animal Husbandry
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
