Effect on the plastid pigments content and grain yield in two maize hybrids following treatment with microbial fertilizers of the “Microbtimа” group

Authors

  • Zhelyazko Vulchinkov Maize Research Institute, 5835 Knezha, Agricultural Academy – Sofia, Bulgaria Author https://orcid.org/0000-0002-1530-0829
  • Miroslava Hristova-Cherbadzhi ROMB Ltd. – Sofia, Bulgaria Author

DOI:

https://doi.org/10.61308/BDUL4627

Keywords:

microbial fertilizers „Microbtima“; plastid pigments; grain yield; maize hybrids

Abstract

Grain yield is a complex trait influenced by a range of secondary morphological and physiological characteristics, including the efficiency of the photosynthetic process. The application of biologically derived products contributes to enhancing overall productivity and the yield potential of cultivated plants, as demonstrated in numerous studies. The aim of the present research was to evaluate the quantitative differences in plastid pigments and their effect on grain yield in maize hybrids Kneja 310 and Kneja 461, following treatment with microbial preparations from the "Microbtima" group. The results showed that, in more than half of the variants for hybrid Kn 310, an increase in chlorophyll content was recorded compared to both control groups, whereas in hybrid Kneja 461, values were generally similar to the controls. The best results were observed with treatment V11 (seed and foliar application), where the content of photosynthetic pigments exceeded that of the controls by 12.64% and 11.21%, respectively. This variant also achieved the highest grain yield (450,42 kg/da) among the 12 tested. For hybrid Kn 461, the highest grain yield (464,48 kg/da) was observed with treatment V9. The rank analysis determined the preparations V11, V13 and V9 as having the most pronounced complex positive effect on the hybrid Kneja 310; and V4 and V9 – on Kneja 461. The bio preparations V9 (Microbtima Stop) and V11 (Microbacterium phyllosphaerae), applied through seed treatment and dual foliar application during vegetation, as promising candidates for further investigation.

References

Anderson, J. M., Chow, W. S., & Park, Y. I. (1995). The grand design of photosynthesis: acclimation of the photosynthetic apparatus to environmental cues. Photosynthesis research, 46(1), 129-139. DOI: https://doi.org/10.1007/BF00020423

Beare, M. H., Coleman, D. C., Crossley Jr, D. A., Hendrix, P. F., & Odum, E. P. (1995). A hierarchical approach to evaluating the significance of soil biodiversity to biogeochemical cycling. Plant and soil, 170(1), 5-22. DOI: https://doi.org/10.1007/BF02183051

Bakonyi, N., Bott, S., Gajdos, E., Szabó, A., Jakab, A., Tóth, B., ... & Veres, S. (2013). Using Biofertilizer to Improve Seed Germination and Early Development of Maize. Polish Journal of Environmental Studies, 22 (6).

Bozova, L., Kerin, V., Kolev, V., Mileva, D., & Ilieva, Т. (1993). Practicum of plant physiology. Academic Edition of the Higher Agricultural Institute, Plovdiv, pp. 24 (Bg).

Breedt, G., Labuschagne, N., & Coutinho, T. A. (2017). Seed treatment with selected plant growth‐promoting rhizobacteria increases maize yield in the field. Annals of Applied Biology, 171(2), 229-236. DOI: https://doi.org/10.1111/aab.12366

Dimova, D., & Marinkov, E. (1999). Experimental work and biometrics. Academic print of AU, 50, 93-98.

dos Santos, R. M., Diaz, P. A. E., Lobo, L. L. B., & Rigobelo, E. C. (2020). Use of plant growth-promoting rhizobacteria in maize and sugarcane: characteristics and applications. Frontiers in Sustainable Food Systems, 4, 136. DOI: https://doi.org/10.3389/fsufs.2020.00136

Evans, J. R. (1989). Photosynthesis and nitrogen relationships in leaves of C3 plants. Oecologia, 78(1), 9-19. DOI: https://doi.org/10.1007/BF00377192

Georgieva, V., Kazandjiev, V., Bozhanova, V., Mihova, G., Ivanova, D., Todorovska, E., ... & Malasheva, P. (2022). Climatic changes—A challenge for the Bulgarian farmers. Agriculture, 12(12), 2090. DOI: https://doi.org/10.3390/agriculture12122090

Hinkina, B., & Angelov, K. (2014). Knezha 310 – A new early maize hybrid. In: Proceeding of the Jubilee conference with international participation - 90 years Maize Research Institute - Kneja, pp. 121- 127 (Bg).

Hristova-Cherbadzhi, M., & Vulchinkov, Z. (2025). Influence of microbial fertilizers from the Microbtima group on the germination of corn hybrid Kneja 310. Bulgarian Journal of Crop Science (In press). DOI: https://doi.org/10.61308/DCDR8471

Jarak, M., Mrkovački, N., Bjelić, D., Jošić, D., Hajnal-Jafari, T., & Stamenov, D. (2012). Effects of plant growth promoting rhizobacteria on maize in greenhouse and field trial. African Journal of Microbiology Research, 6(27), 5683-5690. DOI: https://doi.org/10.5897/AJMR12.759

Kazandjiev, V., Spiridonov, V., & Georgieva, V. (2022). Agrometeorological conditions in the 2021-2050 period and estimates of expected climate change. Journal of Balkan Ecology, 2022, Vol. 25, No. 1, 5-32 ref. 14 ref.

Klok, S., Kornus, A., Kornus, O., Danylchenko, O., & Skyba, O. (2023). Tropical nights (1976–2019) as an indicator of climate change in Ukraine. In IOP Conference Series: Earth and Environmental Science (Vol. 1126, No. 1, p. 012023). IOP Publishing. DOI: https://doi.org/10.1088/1755-1315/1126/1/012023

Kumar, A., Maurya, B. R., Raghuwanshi, R., Meena, V. S., & Tofazzal Islam, M. (2017). Co-inoculation with Enterobacter and Rhizobacteria on yield and nutrient uptake by wheat (Triticum aestivum L.) in the alluvial soil under indo-gangetic plain of India. Journal of plant growth Regulation, 36(3), 608-617. DOI: https://doi.org/10.1007/s00344-016-9663-5

Labuschagne, N., Pretorius, T., & Idris, A. H. (2010). Plant growth promoting rhizobacteria as biocontrol agents against soil-borne plant diseases. In Plant growth and health promoting bacteria (pp. 211-230). Berlin, Heidelberg: Springer Berlin Heidelberg. DOI: https://doi.org/10.1007/978-3-642-13612-2_9

Lalande, R., Bissonnette, N., Coutlée, D., & Antoun, H. (1989). Identification of rhizobacteria from maize and determination of their plant-growth promoting potential. Plant and Soil, 115(1), 7-11. DOI: https://doi.org/10.1007/BF02220688

Lichtenthaler, H. K., & Babani, F. (2021). Contents of photosynthetic pigments and ratios of chlorophyll a/b and chlorophylls to carotenoids (a+ b)/(x+ c) in C4 plants as compared to C3 plants. Photosynthetica, 60(1), 3. DOI: https://doi.org/10.32615/ps.2021.041

Lugtenberg, B., & Kamilova, F. (2009). Plant-growth-promoting rhizobacteria. Annual review of microbiology, 63(1), 541-556. DOI: https://doi.org/10.1146/annurev.micro.62.081307.162918

Mąkosza, A., Nidzgorska-Lencewicz, J., Koźmiński, C., & Michalska, B. (2024). Very warm and tropical nights in voivodeship cities in Poland in the period 1971–2020. Acta Geographica Lodziensia, 117, 53-68. DOI: https://doi.org/10.26485/AGL/2024/117/4

Md Gulzar, A. B., & Mazumder, P. B. (2023). Plant Growth Promoting Phyllobacteria: an Effective Tool for Sustainable Agriculture. Russian Journal of Plant Physiology, 70(8), 196. DOI: https://doi.org/10.1134/S1021443723602355

Meena, R. K., Reddy, K. S., Gautam, R., Maddela, S., Reddy, A. R., & Gudipalli, P. (2021). Improved photosynthetic characteristics correlated with enhanced biomass in a heterotic F1 hybrid of maize (Zea mays L.). Photosynthesis Research, 147(3), 253-267. DOI: https://doi.org/10.1007/s11120-021-00822-6

Noumavo, P., Kochoni, E., Didagbé, Y., Adjanohoun, A., Allagbé, M., Sikirou, R., Gachomo, E., Kotchoni, S., & Baba-Moussa, L. (2013). Effect of Different Plant Growth Promoting Rhizobacteria on Maize Seed Germination and Seedling Development. American Journal of Plant Sciences, 4, 1013-1021. doi: 10.4236/ajps.2013.45125. DOI: https://doi.org/10.4236/ajps.2013.45125

Oleńska, E., Małek, W., Wójcik, M., Swiecicka, I., Thijs, S., & Vangronsveld, J. (2020). Beneficial features of plant growth-promoting rhizobacteria for improving plant growth and health in challenging conditions: A methodical review. Science of the Total Environment, 743, 140682. DOI: https://doi.org/10.1016/j.scitotenv.2020.140682

Parray, J. A., Jan, S., Kamili, A. N., Qadri, R. A., Egamberdieva, D., & Ahmad, P. (2016). Current perspectives on plant growth-promoting rhizobacteria. Journal of Plant growth regulation, 35(3), 877-902. DOI: https://doi.org/10.1007/s00344-016-9583-4

Petrovska, N., & Valkova, V. (2018). Knezha 461-A new maize hybrid from the middle early group. Agricultural Science & Technology (1313-8820), 10(4), 275. DOI: https://doi.org/10.15547/10.15547/ast.2018.04.0

Rippstein, V., de Schrijver, E., Eckert, S., & Vicedo-Cabrera, A. M. (2023). Trends in tropical nights and their effects on mortality in Switzerland across 50 years. PLoS Climate, 2(4), e0000162. DOI: https://doi.org/10.1371/journal.pclm.0000162

Rudolph, N., Labuschagne, N., & Aveling, T. A. S. (2015). The effect of plant growth promoting rhizobacteria on seed germination and seedling growth of maize. Seed Science and Technology, 43(3), 507-518. DOI: https://doi.org/10.15258/sst.2015.43.3.04

Savov, V., Valchinkova, P., Bratkova, S., Angelova, G., Popova, T., & Chakalov, K. (2009). Effect of Microbial, Enzyme and Humic Substanses on Mineral Nutrition and Grain Quality of Maize Hybrids KN 509 And KN M 625. Biotechnology & Biotechnological Equipment, 23(sup1), 905-908 (Bg). DOI: https://doi.org/10.1080/13102818.2009.10818569

Savov, V., Valchinkova, P., Chakalov, K., Popova. T., & Angelova, G. (2011). A Comparative Study for the Impact of Bio Products Containing Humic Substances on Maize Hybrids. Bulgarian Journal of Soil Science, Agrochemistry and Ecology, 45(Supplement 1-4), 257-261 (Bg).

Savov, V., Bratkova, S., Valchinkova, P., Chakalov, K., Popova, T., & Angelova, G. (2013a). Study on the Influence of Rhizospheric Bacteria Bacillus sp. on the Yeld and Quality of Maize Hybrids Kn 435 and Kn 509. Bulgarian Journal of Crop Science, 50(4-5), 25-30 (Bg).

Savov, V., Valchinkova, P., Chakalov, K., & Bratkova, S. (2013b). Research of the Influence of Endomycorrhizal Microflora Glomus sp. and Microfungi Trichoderma sp. on the yield and quality of maize hybrids Kn 435 and Kn 509. Bulgarian Journal of Crop Science, 50(4-5), 31-35 (Bg).

Savov, V., Vulchinkova, P., Popova. T., Chakalov, K., & Angelova, G. (2016). A Study of the Reproductive Response of Two Maize Hybrids Treated with Biohumates and Micro Element Compositions Chelated with Biohumates. In Book of Proceedings Fourth National Conference with International Participation “Humus Substances and their Contribution to the Climate Change Mitigation”, Bulgarian Society of Humic Substances, Sofia, 8-10 September 2016, pp. 370-384 (Bg).

Valchinkova, P., Savov, V., Bratkova, S., Angelova, G., Chakalov, K., & Popova, T. (2013). Stress Tolerance Effect of Biostimulators Treatment on Maize Hybrids. Bulgarian Journal of Crop Science, 50(3), 49-53.

Vejan, P., Abdullah, R., Khadiran, T., Ismail, S., & Nasrulhaq Boyce, A. (2016). Role of plant growth promoting rhizobacteria in agricultural sustainability—a review. Molecules, 21(5), 573. DOI: https://doi.org/10.3390/molecules21050573

Vulchinkova, P., Savov, V., Bratkova, S., Chakalov, K., Angelova, G., & Vulchinkov, S. (2014). Study of Treatment effect with Biostimulators on Morphological Traits, grain yield and its Elements on Maize hybrid Kn 511. In Book of Proceedings of the Jubilee Conference with International Participation “Selection-Genetic and Technological Innovations on Cultivation of Crops” on occasion of celebration of 90th Anniversary of the Maize Research Institute – Knezha, pp. 25-33, IBSN: 978-954-2955-21-4 (Bg).

Vulchinkova, P., Savov, V., Chakalov, K., Popova, T., Angelova, G., & Vulchinkov, Z. (2018). Study of inoculants of bioproducts Voodoo Juice and Tarantula influence on the photosynthetic pigments and water regime of two maize hybrids. In Journal of Mountain Agriculture on the Balkans, Vol. 21, Issue 1, pp. 137–147 (Bg).

Published

28.10.2026

How to Cite

Vulchinkov , Z., & Hristova-Cherbadzhi, M. (2026). Effect on the plastid pigments content and grain yield in two maize hybrids following treatment with microbial fertilizers of the “Microbtimа” group. Bulgarian Journal of Crop Science, 63(5), 19-29. https://doi.org/10.61308/BDUL4627