Mutation breeding in barley at the Institute of Agriculture – Karnobat: Achievements and future directions

Authors

DOI:

https://doi.org/10.61308/GOLC3864

Keywords:

mutation breeding; barley; genetic resources; yield; grain quality

Abstract

Experimental mutagenesis is applied as one of the main methods to broaden genetic diversity in the barley breeding program at the Institute of Agriculture – Karnobat. The aim of this article is to present the achieved results and outline future directions for the application of this approach. The applied mutation breeding schemes are described, focusing on the accelerated development of new genotypes with high breeding value. Some of the obtained morphological mutants are presented, which enrich the set of genetic resources for future breeding activities. Mutant lines with increased yield potential, yield stability across different years, as well as improved grain quality traits are described. Barley varieties developed based on mutation breeding are also presented. Future research directions aimed at increasing yield, improving stress resistance, and enhancing grain technological qualities under changing climate conditions are outlined.

References

Bort, J., Febrero, A., Amaro, T., & Araus, J. L. (1994). Role of awns in ear water-use efficiency and grain weight in barley. Agronomie, 14(2), 133-139.

Dyulgerova, B., & Dyulgerov, N. (2016). Genotype by environment interaction in mutant lines of winter barley for grain yield. Agricultural Science and Technology, 8(1), 14-18.

Dyulgerova, B., & Dyulgerov, N. (2017). Evaluation of high yielding mutants of Hordeum vulgare cultivar Izgrev. Agricultural Science and Technology, 9(2), 103-105.

Dyulgerova, B., & Dyulgerov, N. (2019). Genetic diversity in sodium azide-induced barley mutant lines. Trakia Journal of Sciences, 17(4), 385-391.

Dyulgerova, B., & Dyulgerov, N. (2019). Genotype by environment interaction for grain yield of barley mutant lines. Agriculture (Pol'nohospodárstvo), 65(2), 51-58.

Dyulgerova, B., & Dyulgerov, N. (2020a). Evaluation of advanced mutant lines of winter barley. Bulgarian Journal of Agricultural Science, 26(4), 829–834.

Dyulgerova, B., & Dyulgerov, N. (2020b). Evaluation of hulless mutants of winter barley. Agriculturae Conspectus Scientificus, 85(3), 203-209.

Dyulgerova, B., & Dyulgerov, N. (2020c). Grain quality of mutant lines induced in malting barley varieties. Trakia Journal of Sciences, 18(1), 47-54.

Dyulgerova, B., & Dyulgerov, N. (2020d). Grain yield and yield related traits of sodium azide induced barley mutant lines. Journal of Central European Agriculture, 21(1), 83-91.

Dyulgerova, B., & Dyulgerov, N. (2021). Grain quality of mutant lines of six-rowed barley. Agricultural Science and Technology, 13(1), 19-23.

Dyulgerova, B., & Dyulgerov, N. (2024). Assessment in mutant lines of winter barley using BLUPs. Agricultural Science & Technology, 16(1), 18-26.

Franckowiak, J. D. (1997a). Stock number: BGS 75. Locus name: Awnless. Locus symbol: Lks1. Barley Genetics Newsletter, 26, 112.

Franckowiak, J. D. (1997b). Stock number: BGS 152. Locus name: Hooded lemma 1. Locus symbol: Kap. Barley Genetics Newsletter, 26, 179-180.

Gramatikova, M. (1997). Genetic and Breeding Studies in Barley. Habilitation Thesis for the Award of the Scientific Title "Senior Research Associate First Degree" in Plant Breeding and Seed Production, Karnobat.

Gramatikova, M., & Stefanov, T. (1984). The Use of Mutagenesis in Barley Breeding. Genetics and Breeding, 6, 33–42.

Hansson, M., Youssef, H. M., Zakhrabekova, S., et al. (2024). A guide to barley mutants. Hereditas, 161, 11. https://doi.org/10.1186/s41065-023-00304-w

Kleinhofs, A., Hodgdon, A. L., Owais, W. M., & Nilan, R. A. (1984). Effectiveness and safety of sodium azide mutagenesis. In: Induced mutation for crop improvement in Latin America (Proceedings Regional FAO/IAEA Seminar, Lima, Peru, 1982), 53-58.

Koppolu, R., Jiang, G., Milner, S. G., Muqaddasi, Q. H., Rutten, T., Himmelbach, A., ... & Schnurbusch, T. (2022). The barley mutant multiflorus2. b reveals quantitative genetic variation for new spikelet architecture. Theoretical and Applied Genetics, 1-20.

Liu, C., Frascarelli, G., Stec, A. O., Heinen, S., Lei, L., Wyant, S. R., ... & Morrell, P. L. (2024). Sodium azide mutagenesis induces a unique pattern of mutations. bioRxiv, 2024-05.

Meints, B., Vallejos, C., & Hayes, P. (2021). Multi-use naked barley: A new frontier. Journal of Cereal Science, 102, 103370.

Olivoto, T., Lúcio, A. D. C., Da Silva, J. A. G., Marchioro, V. S., de Souza, V. Q., & Jost, E. (2019). Mean performance and stability in multi-environment trials I: Combining features of AMMI and BLUP techniques. Agronomy Journal, 111(6), 2949–2960.

Olivoto, T., & Lúcio, A. D. C. (2020). Mean: An R package for multi-environment trial analysis. Method in Ecology and Evolution, 11(6), 783–789.

Qin, D. D., Liu, R., Xu, F., Dong, G., Xu, Q., Peng, Y., ... & Li, C. (2023). Characterization of a barley (Hordeum vulgare L.) mutant with multiple stem nodes and spikes and dwarf (msnsd) and fine-mapping of its causal gene. Frontiers in Plant Science, 14, 1189743.

Robert, P., Brault, C., Rincent, R., & Segura, V. (2022). Phenomic selection: A new and efficient alternative to genomic selection. Genomic prediction of complex traits: Methods and protocols, 397-420.

Tomlekova, N. (2010). Induced mutagenesis for crop improvement in Bulgaria. Plant Mutation Reports, 2(2), 4-27.

Published

27.04.2026

How to Cite

Mutation breeding in barley at the Institute of Agriculture – Karnobat: Achievements and future directions. (2026). Bulgarian Journal of Crop Science, 63(2), 41-53. https://doi.org/10.61308/GOLC3864