In vitro Multiplication of Four Species of Genus Goniolimon

Abstract

The species from genus Goniolimon: Goniolimon besserianum (Sсhult. ex Rchb.) Kusn., Goniolimon collinum (Griseb.), Goniolimon dalmaticum (C. Presl) Rchb. f., Goniolimon tataricum (L.) Boiss. are included in the Red Data Book of the Republic of Bulgaria and Annex № 3 of the Biodiversity Act. The aim of the study was to clarify the impact of benzylaminopurine (NAP) on the multiplication factor and to establish at what concentrations the plants showed normal growth. Seeds of the four species were collected in their natural habitats in Bulgaria and used as starting material. One hundred seeds of every species were first sterilized by immersion in 70% ethanol for 40 sec and then surface-sterilized with 30% commercial bleach for 20 min followed by four times rinsing with sterile distilled water. The seeds were cultured in vitro on MS basal salts + 6 g/l agar + 30 g/l sucrose. After two months explants were sub-cultured on the same medium supplemented with different concentrations of BAP (0.1 mg/l, 0.5 mg/l, 1 mg/l and 2 mg/l). The results showed that high multiplication factor (5.58). was obtain for G. besseranum at concentration 0.1 mg/l. BAP At the same concentration good results were obtained for G. collinum (4.75). For G. dalmaticum in comparison to the lower concentrations of BAP best variant was recorded at BAP 0.5 mg/l (5.25). At 1 mg/l BAP in G. tataricum higher multiplication factor (3.6) was achieved.