Factors affecting Camelia japonica L. clonal micropropagation

Abstract

These studies aimed at studying the factors that influence the processes of organogenesis in tissue culture and organs of Camellia japonica in vitro. It is shown that high concentrations of 6-BAP – 2.5 and 3 mg/l cause rapid growth of callus tissues at the base of microcuttings. However, decrease at the concentration of phytohor¬mones: 6-BAP from 2.0 mg/l to 0.5 mg/l in combination with indole acetic acid from 0.5 mg/l to 0.1 mg/l induced the growth of microshoots in WPM medium. It is noted that, variant from the culture medium with phytohormones of cytokinins-and-adenine type (6 BAP and zeatin) at a concentration of 2.0 and 1.0 mg/l, respectively, and 0.1 mg/l of naphthyl acetic acid was the most appropriate for accelerating the growth of microshoots obtained from explants of mixed varieties while the variant of the culture medium containing 6 BAP 2, 0 mg/l, naphthyl acetic acid – 0.5 mg/l and gibberellic acid – 1.0 mg/l was the best for the cultivar Reine des Beautes.