Identification of Bean Viruses in South Central Region of Bulgaria
Keywords:
bean; ELISA; Potyvirus; RT-PCR; virusesAbstract
Bean viruses cause significant damages on quantity and quality of bean production. The aim of the current investigation is to identify the virus species, spread on beans grown in south central region of Bulgaria. The collected leaf samples were analyzed by PTA-, DAS- and TAS-ELISA using serums against poty-, cucumo-, alfamo-, faba- and necroviruses. The most spread appeared to be the potyviruses in single and mixed infection. Bean common mosaic virus (BCMV) was established in significant part of the samples, followed by Bean yellow mosaic virus (BYMV) and Clover yellow vein virus (ClYVV). Cucumber mosaic virus (CMV) was the second most important virus disease of this crop. Alfalfa mosaic virus (AMV) was identified in two samples. A new degenerative primer pair named Poty-B-deg-F/PotyB-deg-R was designed and used in RT-PCR analysis for identification of members of genus Potyvirus. The elaborated primers successfully amplified a single fragment of about 406 bp. The effectiveness of serological and molecular methods for identification of potyviruses was compared.
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