Optimizing the Rooting Process in Propagation in vitro of Cherry Rootstock Gisela 6
Keywords:
auxins; cherry rootstock; Gisela 6; propagation in vitro; rootingAbstract
The possibilities of optimizing the technological cycle during the micropropagation of Cherry rootstock “Gisela” 6 (P. cerasuas “Schattenmorele” × P. canescens sp.) in the step of rooting in vitro were studied. The investigation was carried out in two directions: 1) Following up the effect of the mineral content in the nutrient medium – MS with ¼ macroelements and its modifications concerning the amount of ammonium nitrate; 2) Study on the effect of auxins – IBA, ІАА and NAA at concentration 1.0 mg/l with adding small quantities of GA3. The best conditions for rhizogenesis provide the medium MS, supplemented with IBA (81.5 – 96.3% rooting), achieving optimal performance regarding of mean number and length of formed roots and stem height. When planting rooted plants in ex vitro conditions, a very good transplantation and adaptation was established with active growth of stem segments and leaf mass.
References
Корнова, К., С. Попов, Д. Димитрова. 2008. Проучвания върху in vitro размножаване на черешови сортове. Научни трудове на СУБ – Пловдив, т. VІІ, 302-305
Kitin, P., I. Iliev, A. Scaltsoyiannes, C. Nellas, A. Ru bos, R. Funada. 2005. A comparative histological study be tween normal and fascinated shoots of Prunus avium gener ated in vitro. Plant Cell, Tissue and Organ Culture, 82 (2), 141-150
Lioba, P. and W. Feucht. 1986. Massenvermehrung von Prunrus Cerasus and Prunus avium in vitro. Gartenbauwis senschaft, 50 (6), 268-273
Murashige, T. and E. Scoog. 1962. A revised medium for rapid grouth and bioassays with tobacco tissue cultures. Phisiologia Plantarum, 15, 473-497
Nacheva, L. and P. Gercheva. 2006. The effect of auxin type and concentration on in vitro rooting of Gisels 5 (Cherry dwarf rootstock). Journal of Mountain Agriculture on the Bal kans, vol. 9, 7, 1309-1316
Nacheva, L. and P. Gercheva. 2008. Micropropagation of rootstock for cherry Gisela 6 (Prunus cerasus X Prunus canescens). Journal of Mountain Agriculture on the Balkans, vol. 11, 7, 1569-1581
Nacheva, L. and P. Gercheva. 2009. Micropropagation of Gisels 5 (Cherry dwarf rootstock): The effect of the type and the concentration of the carbohydrates in the nutrient medium. Acta Hort., 825, 261-267
Özzambak, E. and S. Hepaksoy. 1997. Investigations on in vitro proliferation of sour cherry cv. Heimanns Rubin weichsel. Acta Horticulturae, 447, 155-156
Rosati, P., G. Paoli, G. De-Paoli. 1992. Industrial micro propagation of fruit trees and own-rooted plants in Italy. Fruit Belge, 60, 440, 318-328
Ruzic, D. J., V. R. M. Cerovic, L. J. Culafic. 2006. The effect of inheriting factor on mineral nutrition of low vigorous sweet cherry rootstocks. Acta Horticulturae, 725, 385-39
Schmidt, H. and A. Ketzel. 1996. In vitro culture tech niques in sweet cherry breeding. Acta Hort., 410, 111-114
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