In vitro regeneration of tomato cultivars
Keywords:
in vitro cultivation; regeneration; Solanum lycopersicum; tomatoAbstract
The potential for in vitro regeneration of three tomato cultivars using two types of culture media (Rm and Rtaz) was investigated. Two of selected tomato cultivars are cherry type (Aztec and Idul), whereas one of them is indeterminate variety (Buffalo heart). Results showed that the regeneration efficiency varied from 21% to 57%, depending on the cultivar and type of medium. The variety Buffalo heart demonstrated highest potential for regeneration on both cultivation media (50% on Rm and 57% on Rtaz). The lowest regeneration efficiency was counted for variety Idul (21% on Rm and 25% on Rtaz). The impact of the media used on regeneration potential is most significant for the variety Aztec (45% on Rm and 25% on Rtaz).
References
МЗХ (2015a). Ситуационно-перспективен анализ на плодове и зеленчуци.
МЗХ (2015b). Оперативен анализ за основни земеделски култури, Бюлетин № 8/2015
МЗХ (2016). Оперативен анализ за основни земеделски култури, Бюлетин № 46/2016.
Abu-El-Heba, G. A., Hussein, G. M., & Abdalla, N. A. (2008). A rapid and efficient tomato regeneration and transformation system. Landbauforschung volkenrode/ Agriculture and Forestry Research, 58(1/2), 103-110
Ajenifujah-Solebo, S. O. A., Isu, N. A., Olorode, O., Ingelbrecht, I., & Abiade, O. O. (2012). Tissue culture regeneration of three Nigerian cultivars of tomato. Afri can Journal of Plant Science, 6(14), 370-375.
Afroz, A., Chaudhry, Z., Rashid, U., Khan, M. R., & Ali, G. M. (2010). Enhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water. African Journal of Biotechnology, 9(24), 3634-3644.
Ali, A., Yossef, T., & El-Banna, A. (2012). Cytokinin cytokinin interaction ameliorates the callus induction and plant regeneration of tomato (Solanum lycopersicum Mill.). Acta Agronomica Hungarica, 60(1), 47-55.
Ashakiran, K., Sivankalyani, V., Jayanthi, M., Gov indasamy, V., & Girija, S. (2011). Genotype specific shoots regeneration from different explants of tomato (Solanum lycopersicum L.) using TDZ. Asian Journal of Plant Science and Research, 1(2), 107-113.
Bahurupe, J. V., Patil, S. C., Pawar, B. D., Chimote, V. P., & Kale, A. A. (2013). Callus induction and plantlet regeneration in tomato (Solanum lycopersicum L.). Journal of Cell and Tissue Research, 13(2), 3765-3768.
Bhatia, P., & Ashwath, N. (2008). Improving the quality of in vitro culture shoots of tomato. Biotechnology, 7(2), 188-193.
Bhatia, P., Ashwath, N., Senaratna, T., & Midmore, D. (2004). Tissue culture studies of tomato (Lycopersicon esculentum). Plant Cell, Tissue and Organ Culture, 78(1), 1-21.
Chaudry, A., Abbas, S., Yasmin, A., Rashid, H., Ahmed, H. & Anjum, M. A. (2010). Tissue culture studies in tomato (Lycopesricon esculentum) var. moneymaker. Pak J Bot, 42(1), 155-163.
Cruz-Mendívil, A., Rivera-López, J., Germán-Báez, L. J., López-Meyer, M., Hernández-Verdugo, S., López Valenzuela, J. A., Reyes-Moreno, C. & Valdez-Ortiz, A. (2011). A simple and efficient protocol for plant regeneration and genetic transformation of tomato cv. Micro-Tom from leaf explants. HortScience, 46(12), 1655-1660.
Gamborg, O. L., Miller, R., & Ojima, K. (1968). Nutri ent requirements of suspension cultures of soybean root cells. Experimental cell research, 50(1), 151-158.
Gerszberg, A., Hnatuszko-Konka, K., Kowalczyk, T., & Kononowicz, A. K. (2015). Tomato (Solanum lyco persicum L.) in the service of biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC), 120(3), 881-902.
Gudeva, L. K., & Dedejski, G. (2012). In vivo and in vitro production of some genotypes of cherry tomato Solanum lycopersicum var. Cerasiforme (DUNAL). International Journal of Farming and Allied Science, 1(4), 91-96.
Harish, M. C., Rajeevkumar, S., & Sathishkumar, R. (2010). Efficient in vitro callus induction and regenera tion of different tomato cultivars of India. Asian Journal of Biotechnology, 2(3), 178-184.
HORTIbiz Newsletter (2016). http://www.hortibiz.com/ item/news/tomato-production-facts-around-the-world/
Ishag, S., Osman, M. G., & Khalafalla, M. M. (2009). Effects of growth regulators and genotype on shoot regeneration in tomato (Lycopresicon esculentum cv Omdurman). Int J Sustain Crop Prod, 6, 7-13.
Kantor, M. I. H. A. I. L., Sestras, R., & Chowudhury, K. (2010). Identification of the most organogenic-responsive variety of tomato using the variety X medium interac tion. Rom Biotechnol Lett, 5, 5640-5645.
Mensuali-Sodi, A., Panizza, M., & Tognoni, F. (1995). Endogenous ethylene requirement for adventitious root induction and growth in tomato cotyledons and lavandin microcuttings in vitro. Plant Growth Regulation, 17(3), 205-212.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497.
Namitha, K. K., & Negi, P. S. (2013). Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. Arka Ahuti to Plant Growth Regulators. Notulae Scientia Biologicae, 5(2), 220-225.
Plana, D., Fuentes, A., Alvarez, M., Lara, R. M., Alvarez, F., & Pujol, M. (2006). A new approach for in vitro regen eration of tomato plants devoid of exogenous plant growth hormones. Biotechnology Journal, 1(10), 1153-1157.
Rashid, R., & Bal, S. S. (2010). Effect of hormones on di rect shoot regeneration in hypocotyl explants of tomato. Notulae Scientia Biologicae, 2(1), 70-73
Sherkar, H. D., & Chavan, A. M. (2014). Studies on callus induction and shoot regeneration in tomato. Sci Res Rep, 1, 89-93.
Wayase, U. R., & Shitole, M. G. (2014). Effect of plant growth regulators on organogenesis in tomato (Lyco persicon esculentum Mill.) cv. Dhanashri. International Journal of Pure and Applied Sciences and Technology, 20(2), 65-71.
Zhang, W., Hou, L., Zhao, H., & Li, M. (2012). Factors affecting regeneration of tomato cotyledons. Bioscience Methods, 3(4), 27-33.
Tomato Genome Consortium (2012). The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 485(7400), 635-641.
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