А wheat phytocystatin increase resistance to Fusarium oxysporum of а model plant Arabidopsis thaliana
Keywords:
цистатин; TaMDC1; антигъбни свойства; фитопатогенни гъби; ArabidopsisAbstract
Antifungal activities of the wheat cystatin TaMDC1 were analyzed by a heterologous expression of the protein in a model plant Arabidopsis thaliana. The floral buds of Arabidopsis, ecotype Columbia, were transformed in vivo by Agrobacterium tumefaciens using a “Floral dip” method. The putative transformed seeds were selected on MS media supplemented with kanamycin. The integration of the TaMDC1 gene was proved in T1 plants by PCR analyses. Segregation of the gene in T2 and T3 generations was analyzed and homozygous Arabidopsis lines with а single copy of the TaMDC1 gene were selected. Antifungal activities of the TaMDC1 were evaluated by inoculation of plants from selected Arabidopsis lines with phytopathogenic fungus Fusarium оxysporum. All tested lines were more resistant to the pathogen compared to the wild type A. thaliana. The highest resistance to inoculation with F. оxysporum was shown by the lines Cys 9-3-4, Cys 14-2-5 and Cys 18-6-3.
References
Наков, Б., Каров, С., Попов, А., Нешев, Г. (1999). Спе циална фитопатология. PSSA, София
Станчева, Й. (2000). Атлас на болестите по земедел ските култури. 1. Болести по зеленчуковите култу ри. PENSOFT, София-Москва
Станчева, Й. (2002а). Атлас на болестите по земедел ските култури. 3. Болести по полските култури. PENSOFT, София-Москвa
аСтанчева, Й. (2002б). Атлас на болестите по земедел ските култури. 4. Болести по техническите култури. PENSOFT, София-Москва
Carrillo, L., Herrero, I., Cambra, I., Sánchez-Monge, R., Diaz, I. & Martínez, M. (2011). Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms. Plant Physiol Biochem. 49(10), 1191-1200
Cheng, M.-L., Tzen, J.T.C., Shyu, D.J.H. & Chou, W.-M. (2014). Functional characterization of the N-terminal and C-terminal domains of a sesame group II phytocystatin. Botanical Studies 55(1), 18-2
Christova, P.K., Christov, N.K. & Imai, R. (2006). A cold inducible multidomain cystatin from winter wheat inhibits growth of the snow mold fungus, Microdochium nivale. Planta 223, 1207-1218
Christova, P.K., Christov, N.K. Mladenov P.V. & Imai, R. (2018). The wheat multidomain cystatin TaMDC1 displays antifungal, antibacterial, and insecticidal ac tivities in planta. Plant Cell Rep. 37(6), 923-932
Cipriani, G., Fuentes, S., Ballo, V., Salazar, L.F., Ghis lain, M. & Zhang, D.P. (2000). Transgene expression of rice cysteine proteinase inhibitor for the development of resistance against sweet potato feathery mottle virus. Program Repport 1999-2000, International popato cen ter, Lima, Peru: 267-271
Clough, S.J. & Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735-743
Dean, R., J.A. Van Kan, Z.A. Pretorius, K.E. Hammond Kosack, A. Di Pietro, P.D. Spanu, J.J. Rudd,M. Dick man, R. Kahmann, J. Ellis and G.D. Foster (2012). The Top 10 fungal pathogens in molecular plant pathol ogy. Mol. Plant Pathol., 13(4): 414-30
Diop, N.N., Kidric, M., Repellin, A., Gareil, M., d’Arcy Lameta, A., Thi, A.T.P. & Zuily-Fodil, Y. (2004). A multicystatin is induced by drought-stress in cowpea (Vigna unguiculata (L.) Walp.) leaves. FEBS Lett 577, 545-50
Gaddour, K., Vicente-Carbajosa, J., Lara, P., Isabel Lamoneda, I., Díaz, I., & Carbonero, P. (2001). A constitutive cystatin-encoding gene from barley (Icy) responds differentially to abiotic stimuli. Plant molecu lar biology, 45(5), 599-608.
Hines, M. E., Osuala, C. I., & Nielsen, S. S. (1991). Isola tion and partial characterization of a soybean cystatin cysteine proteinase inhibitor of coleopteran digestive proteolytic activity. Journal of agricultural and food chemistry, 39(8), 1515-1520.
Joshi, B.N., Sainani, M.N., Bastawade, K.B., Gupta V.S. & Ranjekar, P.K. (1998). Cysteine protease inhibitor from pearl millet: a new class of antifungal protein. Biochem Biophys Res Commun 246, 382-387
Martinez, M., Lopez-Solanilla, E., Rodriguez-Pelanzu ela, P., Carbonero P. & Diaz, I. (2003). Inhibition of plant-pathogenic fungi by the barley cystatin Hv-CPI (gene Icy) is not associated with its cysteine-proteinase inhibitory properties. Mol Plant Microbe Interact 16, 876-83
Martinez, M., Abraham, Z., Gambardella, M., Echaide, M., Carbonero P. & Diaz, I. (2005). The strawberry gene Cyf1 encodes a phytocystatins with antifungal activity. J Exp Bot 56, 1821-1829
Meinke, D.W., Cherry, J.M., Dean, C., Rounsley, S.D. & Koornneef, M. (1998). Arabidopsis thaliana: a model plant for genome analysis. Science 282, 679-682
Munger, A., Coenen, K., Cantin, L., Goulet, Ch., Vail l ancourt, L.-P., Goulet, M.-C., Tweddell, R., Sains bury, F. & Michaud, D. (2012). Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biology 12, 198-209
Navas-Cortes, J.A., Hau, B. & Jimenez-Diaz, R.M. (2000). Yield loss in chickpeas in relation to develop ment of Fusarium wilt epidemics. Phytopathology 90, 1269-1278
Nganje, W.E., Bangsund, D.A., Leistritz, F.L., Wilson, W.W. & Tiapo, N.M. (2002). Estimating the economic impact of a crop diseases: the case Fusarium head blight in U.S. wheat and barley. National Fusarium head blight forum proceeding, East Lansing: Michigan State Uni versity, 275-281
Pernas, M., Lopez-Solanilla, E., Sanches-Monge, R., Salcedo, G. & Rodrigues-Palenzuela, P. (1999). Anti fungal activity of a plant cystatin. MPMI 12, 624-627
Pernas, M, Sanchez-Monge, R. & Salcedo, G. (2000). Biotic and abiotic stress can induce cystatin expression in chestnut. FEBS Lett 467, 206-10
Pirovani, C.P., Santiago, A. da S., dos Santos, L.S., Micheli, F., Margis, R., Gesteira, A. da S., Alvim, F.C., Pereira, G.A.G. & Cascardo, J.C. de M. (2010). Theobroma cacao cystatins impair Moniliophthora per niciosa mycelial growth and are involved in postponing cell death symptoms. Planta 232(6), 1485-97
Popovic, M.M., Balajic, A., Ristic, D., Krstic, B., Jank ov, M.J. & Gavrovic-Jankulovic, M. (2012). In vitro and in vivo antifungal properties of cysteine protease inhibitor from green kiwifruit. J Sci Food Agric 92, 3072-3078
Purwar, S., Marla, S. S., Singh, U. S., & Kumar, A. (2010). Basal expression studies of cystatins during specific growth stages of wheat spikes for defining their possible role in differential and stage dependent im munity against Karnal bunt (Tilletia indica). Molecular biology reports, 37(3), 1377-1389.
Salmia, M. A. (1980). Inhibitors of endogenous proteinases in Scots pine seeds: fractionation and activity changes during germination. Physiologia Plantarum, 48(2), 266-270.
Senthilkumar, R., Cheng, C. P., & Yeh, K. W. (2010). Genetically pyramiding protease-inhibitor genes for dual broad-spectrum resistance against insect and phy topathogens in transgenic tobacco. Plant biotechnology journal, 8(1), 65-75.
Smyth, D.R. (1990). Arabidopsis thaliana: a model plant for studying the molecular basis of morphogenesis. Aus tralian Journal of Plant Physiology 17, 323-331
Soares-Costa, A., Beltramini, L. M., Thiemann, O. H., & Henrique-Silva, F. (2002). A sugarcane cystatin: recombinant expression, purification, and antifungal activity. Biochemical and biophysical research com munications, 296(5), 1194-1199.
Simova-Stoilova, L., Vaseva, I., Grigorova, B., Demirevska, K. & Feller, U. (2010). Proteolytic activity and cysteine protease expression in wheat leaves under severe soil drought and recovery. Plant Physiology and Biochemistry 48, 200-206
Sugawara, H., Yoshioka, T., Hashiba, T. & Satoh, S. (2002). Antifungal activity of a recombinant carnation cystatin, rDC-CPIn. Plant Biotechnology 19, 207-209
Urwin, P.E., Lilley, C.J., McPherson, M.J. & Atkinson, H.J. (1997). Resistance to both cyst and root-knot nema todes conferred by transgenic Arabidopsis expressing a modified plant cystatin. Planta J 12, 455-461.
Valdes-Rodriguez, S., Cedro-Tanda, A., Aguilar Hernandez, V., Cortes-Onofre, E., Blanco-Labra, A. & Guerrero-Rangel, A. (2010). Recombinant amaranth cystatin (AhCPI) inhibits the growth of phytopathogenic fungi. Plant Physiol. Biochem. 48, 469-475
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2018 Bulgarian Journal of Crop Science
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
