Influence on primed with cytokinins seeds of common winter wheat (Triticum aestivum L.) over their vitality in modeled accelerated ageing
Keywords:
accelerated ageing; antioxidant enzyme activities; common winter wheat; seed germinationAbstract
Seeds from contrasting wheat cultivars (Sadovo 772 and Geya-1) differing in productivity and composition were subjected to modeled accelerated aging under laboratory conditions for 72 h. Before induced aging seeds were soaked in cytokinin solutions (either 6-BA or kinetin). After cytokinin treatment seeds were germinated for 5 days and emerging coleoptiles were used for analysis of some anti-stress parameters and anti-oxidative response-malondialdehyde, hydrogen peroxide, cell membrane stability, free proline, soluble phenols and thiols groups. The aim of the study was to assess the relationship between germination of seeds pre-treated with cytokinin and their stress response to induced accelerated aging. It was concluded that the cultivars with lower productivity and correspondingly lower protein containing seeds demonstrated faster aging and worse germination capacity. Delayed coleoptile development was associated with oxidative stress due to higher hydrogen peroxide and malondialdehyde accumulation. Seed pre-treatment with cytokinins improved coleoptile growth through reducing malondialdehyde and hydrogen peroxide formation and thus enhancing cell membrane stability. Growth was also promoted through the accumulation of free proline, phenols and free thiols groups.
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