Role of β-carotene on alleviation of salt-induced stress in Allium cepa L.
Keywords:
Allium cepa L.; mitotic index; salt stress; seed germination; seedling growth; β-carotene, chromosomal aberrationsAbstract
Effects of β-carotene on the seedling growth (fresh weight, radicle length and radicle number), seed germination, chromosomal aberrations (CAs) and mitotic index (MI) in Allium cepa L. germinated in both normal and salt stress conditions were investigated in this study. In only β-carotene medium, the fresh weight and radicle number of the seedling were reduced partially compared to the control seedling grown in the distilled water medium. While their germination percentage indicated statistically the same values as the control, their radicle number was decreased according the control. Besides, the MI in the root tip meristematic cells of Allium cepa seeds germinated in alone β-carotene medium statistically showed the same value compared to the control seeds germinated in the distilled water medium, whereas the CAs exhibited increase significantly compared to the control. On the other hand, salt stress significantly inhibited the seedling growth and seed germination of A. cepa. What’s more, it markedly reduced the MI in the root tip meristems of the seeds and increased the number of CAs. Nonetheless, inhibitive effects of salt on the MI, seedling growth, seed germination and CAs significantly decreased with the application of β-carotene.
References
Ali, R. M. (2000). Role of putrescine in salt tolerance of Atropa belladonna plant. Plant Science, 152, 173-179.
Allende Martínez, L. M. (1997). Effects of retinol (vitamin A) in human T lymphocyte activation and therapeutic implications. Dissertation, Spain: Madrid Complutense Univ.
Amer, S. M. & Alı, E. M. (1974). Cytological effects of pesticides. V. effects of some herbicides on Vicia faba. Cytologia, 39, 633-643.
Ateeq, B., Abul Farah, M., Niamat Ali, M. & Ahmad, W. (2002). Clastogenicity of pentachlorophenol, 2, 4-D and butachlor evaluated by Allium root tip test. Mutation Research, 514, 105-113.
Benítez-García, I., Vanegas-Espinoza, P. E., Meléndez-Martínez, A. J., Heredia, F. J., Paredes-López, O. & Villar-Martínez, A. A. D. (2014). Callus culture development of two varieties of Tagetes erecta and carotenoid production. Electronic Journal of Biotechnology, 17, 107–113.
Burton, G. W. & Ingold, K. U. (1984). Beta-carotene: an unusual type of lipid antioxidant. Science, 224, 569-573.
Cazzonelli, C. I. (2011). Carotenoids in nature: insights from plants and beyond. Functional Plant Biology, 38, 833-847.
Çavuşoğlu, D. (2020a). Role of propolis in alleviation of detrimental effects of salt stress on some physiological and cytogenetical parameters in onion (Allium cepa L.). Bulgarian Journal of Crop Science, 57(2), 26-31.
Çavuşoğlu, D. (2020b). The effects of Green Tea Leaf Extract on cytogenetical and physiological parameters of Allium cepa L. exposed to salt salinity. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi (SAUJS), 24(2), 338-346.
Çavuşoğlu, K., Cadıl, S. & Çavuşoğlu, D. (2017). Role of potassium nitrate (KNO3) in alleviation of detrimental effects of salt stress on some physiological and cytogenetical parameters in Allium cepa L. Cytologia, 82(3), 279-286.
Çavuşoğlu, K., Doğu, F. & Çavuşoğlu, D. (2019). Effects of Sodium Hypochlorite (NaClO) on some Physiological and Cytogenetical Parameters in Allium cepa L. Exposed to Salt Stress. Bangladesh Journal of Botany, 48(2), 223-229.
Diretto, G., Al-Babili, S., Tavazza, R., Papacchioli, V., Beyer, P. & Giauliano, G. (2007). Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway. BMC Plant Biology, 2(4), 350.
Fiskesjö, G. (1979). Two organic mercury compounds tested for mutagenicity in mammalian cells by use of cell line V-79-4. Heredıtas, 90, 103-109.
Gari, S. H., Sabir, J. S. & Baeshin, N. A. (1998). Cytotoxic and genotoxic effects of cadmium chloride in root meristems of Vicia faba. Proc. 6 th International Congress on Molecular Genetic, 1, 95–100.
Gisselsson, D., Palsson, E., Yu, C., Mertens, F. & Mandahl, N. (2004). Mitotic instability associated with late genomic changes in bone and soft tissue tumours. Cancer Letters, 206, 69–76.
Jackson, H., Braun, C. L. & Ernst, H. (2008). The chemistry of novel xanthophyll carotenoids. American Journal of Cardiology, 101, 50–57.
Just, B.J., Santos, C. A. F., Fonseca, M. E. N., Boiteux, L. S., Oloizia, B. B. & Simon, P. W. (2007). Carotenoid biosynthesis structural genes in carrot (Daucus carota): Isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping. Theoretical and Applied. Genetics, 114, 693–704.
Kara, M., Tanda, M .A. & Ateş, A. (1994). Cytogenetic effects of the insecticide cypermethin on the root meristems of Allium cepa L. Turkish Journal of Biology, 18, 323–331.
Kuiper, D., Schuit, J. & Kuiper, P. J. C. (1990). Actual cytokinin concentrations in plant issue as an indicator for salt resistance in cereals. Plant and Soil, 123, 243-250.
Leme, D. M. & Marin-Morales, M. A. (2009). Allium cepa test in environmental monitoring: a review on its application. Mutation Research, 682, 71–81.
Leung, A. Y. (1980). Encyclopedia of Common Natural Ingredients used in food, drugs and cosmetics, B49: Wiley, 848.
Majewska, A., Kuraṡ, M. & Furmanowa, M. (2002). Influence of extract from shoots of Taxus baccata var. Elegantissima on tubulin cytoskleton and ultrastructure of meristematic cells of Allium cepa L. roots. Acta Societatis Botanicorum Poloniae, 3, 211-221.
Mccue, K .F. & Hanson, A. D. (1990). Drought and salt tolerance: towards understanding and application. Trends Biotechnologgy, 8, 358-362.
Morales-González, J. A. (2009). Antioxidant defenses: Antioxidants and chronic degenerative diseases, Miranda Martínez, I., Gasca León, M.I., Aedo Santos, M.A., Cantoral Preciado, A.J., Gallardo Wong, I., Eds., Hidalgo: México, 131-225.
Nefic, H., Musanovic, J., Metovic, A. & Kurteshi, K. (2013). Chromosomal and nuclear alterations in root tip cells of Allium cepa L. induced by alprazolam. Medical Arhives, 67, 388–392.
Polívka, T. & Frank, H. A. (2010). Molecular factors controlling photosynthetic light harvesting by carotenoids. Accounts of Chemical. Research, 43, 1125–1134.
Radic, S., Prolic, M., Pavlica, M. & Pevalek-Kozlina, B. (2005). Cytogenetic effects of osmotic stress on the root meristem cells of Centaurea ragusina L. Environmental and Experimental Botany, 54, 213-218.
Rhoades, J. D. & Loveday, J. (1990). Salinity in irrigated agriculture: Steward, B.A. and Nielsen, D.R., Eds., Am. Soc. of Civil Engineers/Agronomists. Irrigation of Agricultural. Crops, 30, 1089–1142.
Singh, R. J. (2003). Plant cytogenetics, Boca Raton (US): CRC Press, 133.
Slinger, L. A. & Bird, G. W. (1978). Ontogeny of Daucus carota infected with Meloidogyne hapla. Journal of Nematology, 10(2), 188-194.
Türkoglu, S. (2007). Genotoxicity of five food preservatives tested on root tips of Allium cepa L. Mutation Research, 626, 4–14.
Udengwu, O. S. (2012). Effects of aluminium induced stress, and treatments wıth two lıpophılc antıoxıdants, on germınatıon, growth and proteın profıle of Amaranthus hybrıdus. Dissertation, Nigeria: Nigeria Nsukka Univ.
Waters, J. C. & Salmon, E. D. (1997). Pathways of spindle assembly. Current Opinion in Cell Biology, 9, 37-43.
Winter-Griffith, H. (1988). The vital vitamin fact file, B24: Thorsons publishing, 40.
Zhu, J. K. (2001). Plant salt tolerance. Trends Plant Sciences, 6, 66–71.
Zhu, J. K. (2002). Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53, 247–273.
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